Artemisia gmelinii Attenuates Lung Inflammation by Suppressing the NF-κB/MAPK Pathway

Kim, Seung Yong; Shin, Dong‐Uk; Eom, Ji-Eun; Song, Hyeon-Ji; Lim, Kyung Min; Kim, Gun-Dong; Yun, Soon-Il; Kim, Mi‐Yeon; Shin, Hee Soon; Lee, So‐Young

doi:10.3390/antiox11030568

Cited by 8 publications

(5 citation statements)

References 55 publications

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“…Artemisia gmelinii Weber ex Stechm (AG) contains various bioactive compounds such as flavonoids, terpenoids, coumarins, caffeoylquinic acids, sterols, and acetylenes. In an earlier study, we demonstrated the beneficial effects of AGE in a respiratory disease model for COPD and allergic airway inflammation [ 20 , 41 ]. Additionally, the present study suggests that AGE effectively inhibits PM-induced lung damage and pulmonary inflammatory responses by suppressing the NF-κB/MAPK pathway and neutrophil infiltration.…”

Section: Discussionmentioning

confidence: 99%

“…It exerts various biological influences, such as antioxidant, anti-inflammatory, and anti-allergic effects [ 17 , 18 , 19 ]. In addition, our previous study showed that AG ethanol extract (AGE) decreased the release of inflammatory cytokines and chemokines, inhibited MAPK and NF-κB signaling pathways to alleviate lung inflammation in a COPD mouse model, and attenuated inflammation in cigarette smoke extract (CSE)/lipopolysaccharide (LPS)-treated mouse alveolar macrophages (MH-S) [ 20 ]. Nonetheless, the therapeutic effects of AGE on lung inflammation have yet to be demonstrated in a model of PM-induced lung injury.…”

Section: Introductionmentioning

confidence: 99%

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Artemisia gmelinii Extract Attenuates Particulate Matter-Induced Neutrophilic Inflammation in a Mouse Model of Lung Injury

et al. 2023

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Particulate matter (PM) induces and augments oxidative stress and inflammation, leading to respiratory diseases. Although Artemisia gmelinii Weber ex Stechm has antioxidant and anti-inflammatory effects, there are no reports on whether Artemisia gmelinii extract (AGE) regulates lung inflammation in a PM-induced model. Thus, we investigated the protective effects of AGE using a PM-induced mouse lung inflammation model. AGE significantly decreased the expression of inflammatory chemokines, neutrophil extracellular trap formation, and the total number of inflammatory cells in the bronchoalveolar lavage fluid (BALF). Furthermore, AGE attenuated lung inflammation through the suppression of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)/mitogen-activated protein kinase (MAPK) signaling pathway, while promoting the nuclear factor erythroid-2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) signaling pathway in lung tissues. Concordant with these observations, AGE suppressed inflammatory cytokines, chemokines, reactive oxygen species, NETosis, myeloperoxidase, and neutrophil elastase by decreasing the mRNA expression of High mobility group box 1, Runt-related transcription factor 1, and Kruppel-like factor 6 in differentiated HL-60 cells. In summary, our data demonstrated that AGE suppresses PM-induced neutrophil infiltration, lung damage, and pulmonary inflammation by suppressing NF-κB/MAPK signaling pathways and enhancing the NRF2/HO-1 signaling pathway. These findings suggest that AGE administration is an effective approach for preventing and treating PM-induced respiratory inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Artemisia gmelinii Extract Attenuates Particulate Matter-Induced Neutrophilic Inflammation in a Mouse Model of Lung Injury

et al. 2023

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“…To set the COPD model, mice were intranasally treated with 20 μL of PPE (1.2 U/head) once a week and CSE (100%) three times a week from days 7 to 18. We decided the dose of PPE/CSE by referring to following references presenting typical COPD symptoms such as airway inflammation, mucus overproduction, and emphysema [ 26 , 27 ]. The COPD and CLE groups were given 200 μL/mouse of PBS or CLE diluted in PBS from days 0 to 18.…”

Section: Methodsmentioning

confidence: 99%

Camellia sinensis L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract

et al. 2022

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Cigarette smoke (CS) is the major factor in the development of chronic obstructive pulmonary disease (COPD), the third leading cause of death worldwide. Furthermore, although Camellia sinensis (CN) has been known as an anti-inflammatory material, the effect of CN has not yet been known on pulmonary inflammation in COPD. Thus, we investigated the protective effects of Camellia sinensis L. extract (CLE) against pulmonary inflammation in porcine pancreas elastase (PPE) and a cigarette smoke extract (CSE)-induced COPD mouse model. Oral administration of CLE suppressed the symptoms such as infiltration of immune cells, cytokines/chemokines secretion, mucus hypersecretion, and injuries of the lung parenchyma. Increased inflammatory responses in COPD are mediated by various immune cells such as airway epithelial cells, neutrophils, and alveolar macrophages. Thus, we investigated the effect and mechanisms of CLE in H292, HL-60, and MH-S cells. The CLE inhibited the expression of IL-6, IL-8, MUC5AC and MUC5B on CSE/LPS-stimulated H292 cells and also suppressed the formation of neutrophil extracellular traps and secretion of neutrophil elastase by inhibiting reactive oxygen species in PMA-induced HL-60 cells. In particular, the CLE suppressed the release of cytokines and chemokines caused by activating the nuclear factor kappa-light-chain-enhancer of activated B via the activation of nuclear factor erythroid-2-related factor 2 and the heme oxygenase-1 pathway in CSE/LPS-stimulated MH-S cells. Therefore, we suggest that the CLE administration be the effective approach for treating or preventing chronic pulmonary diseases such as COPD induced by CS.

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“…The concentrations of the extracted proteins were measured using the Bradford protein assay. Protein expression was determined by western blot analysis using a previously published method [25,26]. The expression of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) and β-actin (Cell Signaling Technology, USA) in the cytoplasmic fraction, and nuclear factor kappa-light-chain-enhancer of activated B cell (NFκB), p65, and lamin B1 (Cell Signaling Technology) in the nuclear fraction, were measured using the ChemiDoc XRS+ (Bio-Rad, USA).…”

Section: Peritoneal Macrophage Collection and Western Blot Analysismentioning

confidence: 99%

“…COPD was induced using CSE and PPE based on a previously established COPD mouse model [24][25][26]. Oral administration of LLT and LS803 was initiated 7 days before CSE and PPE induction to identify the prophylactic effects.…”

Section: Effect Of Ls803 On Inflammatory Cell Infiltration Cytokines ...mentioning

confidence: 99%

Bulb of Lilium longiflorum Thunb Extract Fermented with Lactobacillus acidophilus Reduces Inflammation in a Chronic Obstructive Pulmonary Disease Model

Eom

Kim

et al. 2023

J. Microbiol. Biotechnol.

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Chronic obstructive pulmonary disease (COPD), one of the leading causes of death worldwide, is caused by repeated exposure to harmful matter, such as cigarette smoke. Although Lilium longiflorum Thunb (LLT) has anti-inflammatory effects, there is no report on the fermented LLT bulb extract regulating lung inflammation in COPD. Thus, we investigated the protective effect of LLT bulb extract fermented with Lactobacillus acidophilus 803 in COPD mouse models induced by cigarette smoke extract (CSE) and porcine pancreas elastase (PPE). Oral administration of the fermented product (LS803) suppressed the production of inflammatory mediators and the infiltration of immune cells involving neutrophils and macrophages, resulting in protective effects against lung damage. In addition, LS803 inhibited CSE-and LPS-induced IL-6 and IL-8 production in airway epithelial H292 cells as well as suppressed PMA-induced formation of neutrophil extracellular traps in HL-60 cells. In particular, LS803 significantly repressed the elevated IL-6 and MIP-2 production after CSE and LPS stimulation by suppressing the activity of the nuclear factor kappa-light-chainenhancer of activated B (NFκB) in mouse peritoneal macrophages. Therefore, our results suggest that the fermented product LS803 is effective in preventing and alleviating lung inflammation.

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Artemisia gmelinii Attenuates Lung Inflammation by Suppressing the NF-κB/MAPK Pathway

Cited by 8 publications

References 55 publications

Artemisia gmelinii Extract Attenuates Particulate Matter-Induced Neutrophilic Inflammation in a Mouse Model of Lung Injury

Artemisia gmelinii Extract Attenuates Particulate Matter-Induced Neutrophilic Inflammation in a Mouse Model of Lung Injury

Camellia sinensis L. Alleviates Pulmonary Inflammation Induced by Porcine Pancreas Elastase and Cigarette Smoke Extract

Bulb of Lilium longiflorum Thunb Extract Fermented with Lactobacillus acidophilus Reduces Inflammation in a Chronic Obstructive Pulmonary Disease Model

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