Artemisia ordosica Polysaccharide Alleviated Lipopolysaccharide-induced Oxidative Stress of Broilers via Nrf2/Keap1 and TLR4/NF-κB Pathway

Xing, Yuanyuan; Zheng, Y.K.; Yang, Shuo; Zhang, L.H.; Guo, Shiwei; Shi, Lei; Xu, Yuanqing; Xiao, Jin; Yan, Sumei; Shi, Binlin

doi:10.1016/j.ecoenv.2021.112566

Cited by 50 publications

(32 citation statements)

References 30 publications

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“…Similarly, Zhang et al (36) found that the nuclear translocation of p65 was also significantly inhibited by Artemargyinolide E (a new sesquiterpene lactone from Artemisia argyi) in vitro. Moreover, a previous study showed that inflammation could be regulated via the TLR-4/NF-κB signaling pathway in mice and broilers (37,38). Thus, based on the results of this study, we preliminarily speculated that AAE could reduce the content and gene expression level of IL-1β and IL-6 in the intestinal mucosa of broilers by regulating the TLR4/NF-κB signaling pathway.…”

Section: Discussionmentioning

confidence: 63%

“…In keeping with our findings, Xing et al (41) reported that Artemisia ordosica polysaccharide could improve the antioxidant capacity of rats by upregulating the gene expression level of SOD, CAT, and GPx. Simultaneously, another study showed that Artemisia ordosica polysaccharide could increase the activity of the antioxidant enzyme in liver of broilers and improve antioxidant status through the Nrf2/Keap1 pathway (38). Moreover, some studies manifested that A. annua extract upregulated the mRNA expression of GPx and SOD in small intestine of broilers, which was consistent with the results of antioxidant-related enzyme activity, meanwhile, and the mRNA expression of Nfe2l2 and Hmox1 was following the protein expression of Nrf2 and HO-1, which indicated that A. annua extract improved intestinal antioxidant capacity by activating the related mRNA and protein expression of the Nrf2/AREmediated pathway (26).…”

Section: Discussionmentioning

confidence: 96%

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Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Guo

Xing

et al. 2022

Front. Vet. Sci.

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This study was conducted to investigate the effects of Artemisia annua L. aqueous extract (AAE) on intestinal immune and antioxidative function of broilers. A total of 200 one-day-old Arbor Acre broilers were randomly allotted into five dietary treatment groups, with five replicates per treatment and eight broilers per replicate. The five treatment diets were formulated by adding, respectively, 0 (control group), 0.5, 1.0, 1.5, and 2.0 g/kg AAE in the basal diet. The results showed that dietary inclusion of AAE quadratically decreased interleukin (IL)-1β content, linearly decreased IL-6 content in the small intestine through regulating the nuclear factor-kappa B signal pathway, and quadratically increased immunoglobulin (Ig)M and sIgA content in ileum and jejunum. Besides, there was a quadratic decrease in the gene expression of IL-1β, IL-6, and toll like receptor 4 (TLR4) in ileum on day 21, and the gene expression of IL-6 and TLR4 in duodenum on day 42, thereby improving small intestinal immune function in broilers. Additionally, dietary inclusion of AAE improves antioxidative function through the nuclear factor-erythroid 2-related factor 2 (Nrf2) signal pathway in the small intestinal mucosa of broilers, especially, quadratically increased catalase (CAT) and superoxidase dismutase activity in ileum, and total antioxidant capacity and glutathione peroxidase activity in duodenum, and quadratically decreased malondialdehyde concentration in ileum, besides, linearly increased heme oxygenase-1 and Nrf2 gene expression in jejunum and ileum on day 42, quadratically increased CAT gene expression in the small intestine. Furthermore, regression analyses of the above parameters showed that the optimal dose range of AAE in the diet of broilers was 1.12–1.38 g/kg.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 96%

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Guo

Xing

et al. 2022

Front. Vet. Sci.

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“…Noteworthily, there were four antioxidant enzyme-related genes (SOD2, GPx1, GSTT1, and GSTO1) whose expression was adversely affected by HS exposure, but only two of them (SOD2 and GSTO1) were significantly reverted after feeding SDP. Likewise, other evidence supported that the natural polysaccharides regulate only certain antioxidant genes in chickens [13,57] and rats [58]. Huang et al [59] demonstrated that a dietary low dose of plant-derived polysaccharides only improved the mRNA expression of jejunal SOD2 and GSTP1 and increasing the polysaccharides intake could significantly upregulate the mRNA expression of SOD1, SOD2, GSTP1, and GSTM1 in a mice model of aging.…”

Section: Discussionmentioning

confidence: 97%

Seaweed-Derived Polysaccharides Attenuate Heat Stress-Induced Splenic Oxidative Stress and Inflammatory Response via Regulating Nrf2 and NF-κB Signaling Pathways

Zhuang

Zhao

et al. 2022

Marine Drugs

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With global warming, heat stress (HS) has become a worldwide concern in both humans and animals. The ameliorative effect of seaweed (Enteromorpha prolifera) derived polysaccharides (SDP) on HS-induced oxidative stress and the inflammatory response of an immune organ (spleen) was evaluated using an animal model (Gallus gallus domesticus). In total, 144 animals were used in this 4-week trial and randomly assigned to the following three groups: thermoneutral zone, HS, and HS group supplemented with 1000 mg/kg SDP. Dietary SDP improved the antioxidant capacity and reduced the malondialdehyde (MDA) of the spleen when exposed to HS, regulated via enhancing nuclear factor erythroid 2-related factor-2 (Nrf2) signaling. Furthermore, the inclusion of SDP reduced the levels of pro-inflammatory cytokines and alleviated HS-induced splenic inflammatory response by suppressing the nuclear factor-kappa B (NF-κB) p65 signaling. These findings suggest that the SDP from E. prolifera can be used as a functional food and/or feed supplement to attenuate HS-induced oxidative stress and inflammatory responses of the immune organs. Moreover, the results could contribute to the development of high-value marine products from seaweed for potential use in humans and animals, owing to their antioxidant and anti-inflammatory effects.

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“…Western blot was conducted for determining the liver protein expression level related to NF-κB signaling pathway. Four replicates per treatment were randomly selected, which was referred to previous studies ( Hollemans et al., 2021 ; Xing et al., 2021 ). Cytoplasmic protein and nuclear protein were obtained using the Nuclear and Cytoplasmic Protein Extraction kit (Beyotime Biotechnology, Shanghai, China) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Trans-anethole ameliorates lipopolysaccharide-induced acute liver inflammation in broilers via inhibiting NF-κB signaling pathway

Tong

Xie

et al. 2022

Poultry Science

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Artemisia ordosica Polysaccharide Alleviated Lipopolysaccharide-induced Oxidative Stress of Broilers via Nrf2/Keap1 and TLR4/NF-κB Pathway

Cited by 50 publications

References 30 publications

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Seaweed-Derived Polysaccharides Attenuate Heat Stress-Induced Splenic Oxidative Stress and Inflammatory Response via Regulating Nrf2 and NF-κB Signaling Pathways

Trans-anethole ameliorates lipopolysaccharide-induced acute liver inflammation in broilers via inhibiting NF-κB signaling pathway

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