Artemisinin inhibits the proliferation, migration, and inflammatory reaction induced by tumor necrosis factor-α in vascular smooth muscle cells through nuclear factor kappa B pathway

Cao, Qian; Jiang, Yan; Shi, Jin; Xu, Changlu; Liu, Xiaoxia; Yang, Tiangui; Fu, Peng; Niu, Tiesheng

doi:10.1016/j.jss.2014.12.013

Cited by 41 publications

(25 citation statements)

References 33 publications

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“…In addition, the expression levels of cyclin D1, cyclin E, CDK2, and CDK4 were downregulated by ART in a dose‐dependent manner, resulting in blocking the G0/G1 and G1/S checkpoint; this may eventually induce G1 phase cell cycle arrest and inhibit the proliferation of TNF‐ α ‐stimulated VSMC. We previously confirmed that ART inhibits the proliferation, migration, and inflammatory reaction induced by TNF‐ α in VSMC through the nuclear factor kappa B pathway . Moreover, it has also been reported that ART attenuates lipopolysaccharide‐stimulated pro‐inflammatory responses by inhibiting the nuclear factor kappa B pathway in microglia cells .…”

Section: Discussionsupporting

confidence: 64%

Artemisinin inhibits tumour necrosis factor‐α‐induced vascular smooth muscle cell proliferation in vitro and attenuates balloon injury‐induced neointima formation in rats

Cao

Jiang

Shi

et al. 2015

Clin Exp Pharma Physio

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The aim of this study was to evaluate the effect of artemisinin (ART) on rat vascular smooth muscle cell (VSMC) proliferation induced by tumour necrosis factor (TNF)-α, cell cycle arrest, and apoptosis, and its effect on neointima formation after balloon injury of rat carotid artery. Primary rat VSMC were identified by immunofluorescence assay. The proliferation of VSMC induced by TNF-α was significantly inhibited by ART treatment in a dose-dependent manner. Treatment with 100-μM ART significantly reduced the expression of proliferating cell nuclear antigen. In contrast, the same treatment arrested the cell cycle in G0/G1 phase. Western blot analysis showed that the cell cycle-related proteins cyclin D1, cyclin E, cyclin-dependent kinase 2, and cyclin-dependent kinase 4 were downregulated by ART in TNF-α-stimulated VSMC. For apoptosis induced by ART, cleaved caspase-3/-9 was detected, and the pro-apoptotic protein Bcl-2-associated X protein was upregulated while the anti-apoptotic protein Bcl-2 was downregulated. The results suggest that ART can effectively inhibit the proliferation of VSMC induced by TNF-α through the apoptotic induction pathway and cell cycle arrest. Also, balloon injury indicated that ART significantly inhibited neointima formation in the rat carotid arteries.

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Section: Discussionsupporting

confidence: 64%

Artemisinin inhibits tumour necrosis factor‐α‐induced vascular smooth muscle cell proliferation in vitro and attenuates balloon injury‐induced neointima formation in rats

Cao

Jiang

Shi

et al. 2015

Clin Exp Pharma Physio

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“…Inflammatory cytokines, including TNF- α , are known to trigger invasion of HASMCs mediated by matrix metalloproteinases [16, 17]. Our results here show that treatment with ZNE (100 and 150 μ g/ml) inhibited HASMC invasion of the Matrigel-coated filter inserts.…”

Section: Resultssupporting

confidence: 51%

Ziziphus nummularia Inhibits Inflammation‐Induced Atherogenic Phenotype of Human Aortic Smooth Muscle Cells

Fardoun

Al-Shehabi²,

El-Yazbi

et al. 2017

Oxidative Medicine and Cellular Longevity

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Cardiovascular disease (CVD) continues to be the leading cause of death worldwide. Atherosclerosis is a CVD characterized by plaque formation resulting from inflammation-induced insults to endothelial cells, monocytes, and vascular smooth muscle cells (VSMCs). Despite significant advances, current treatments for atherosclerosis remain insufficient, prompting the search for alternative modalities, including herbal medicine. Ziziphus nummularia is an herb commonly used in the amelioration of symptoms associated with many health conditions such as cold, diarrhea, cancer, and diabetes. However, its effect on the inflammation-induced behavior of VSMCs remains unknown. In this study, we sought to determine the effect of the ethanolic extract of Z. nummularia (ZNE) on TNF-α-induced phenotypic changes of human aortic smooth muscle cells (HASMCs). The treatment of HASMCs with ZNE decreased cell proliferation, adhesion to fibronectin, migration, and invasion. ZNE treatment also caused a concentration- and time-dependent reduction in the TNF-α-induced expression of matrix metalloproteases MMP-2 and MMP-9, NF-κB, and cell adhesion molecules ICAM-1 and VCAM-1. Furthermore, ZNE decreased the adhesion of THP-1 monocytes to HASMCs and endothelial cells in a concentration-dependent manner. These data provide evidence for the anti-inflammatory effect of Ziziphus nummularia, along with potential implications for its use as an agent that could ameliorate inflammation-induced atherogenic phenotype of VSMCs in atherosclerosis.

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“…in their 2014 study that reported a similar depletion of CD4 + CD25 + FOXP3 + Treg in mouse cervical cancer models, with a corresponding inhibition of PGE 2 (prostaglandin E2) production and FOXP3 downregulation . This reduction in PGE 2 corroborates with a subsequent study in smooth muscle cells . Changes in signaling molecule production however is likely to be cell‐line dependent, as demonstrated by Cui et al.…”

Section: Molecular Targets and Signaling Pathways Implicated In The Amentioning

confidence: 99%

Artemisinin as an anticancer drug: Recent advances in target profiling and mechanisms of action

Wong

Kalesh

et al. 2017

Medicinal Research Reviews

201

139

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Artemisinin inhibits the proliferation, migration, and inflammatory reaction induced by tumor necrosis factor-α in vascular smooth muscle cells through nuclear factor kappa B pathway

Cited by 41 publications

References 33 publications

Artemisinin inhibits tumour necrosis factor‐α‐induced vascular smooth muscle cell proliferation in vitro and attenuates balloon injury‐induced neointima formation in rats

Artemisinin inhibits tumour necrosis factor‐α‐induced vascular smooth muscle cell proliferation in vitro and attenuates balloon injury‐induced neointima formation in rats

Ziziphus nummularia Inhibits Inflammation‐Induced Atherogenic Phenotype of Human Aortic Smooth Muscle Cells

Artemisinin as an anticancer drug: Recent advances in target profiling and mechanisms of action

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