Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Wanderer, Stefan; Waltenspuel, Claudia; Grüter, Basil E.; Strange, Fabio; Sivanrupan, Sivani; Remonda, Luca; Widmer, Hans Rudolf; Casoni, Daniela; Andereggen, Lukas; Fandiño, Javier; Marbacher, Serge

doi:10.3791/61157

Cited by 8 publications

(8 citation statements)

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“…This study was designed as prospective observational trial. Data were collected from animals used for another study aimed to develop a model of microsurgical carotid artery bifurcation aneurysms [29,30].…”

Section: Methodsmentioning

confidence: 99%

“…The number of rabbits undergoing general anesthesia, for both clinical and experimental reasons is increasing each year. In biomedical research, these animals often undergo neurovascular surgeries that may lead to an impairment in cerebral blood supply [29][30][31][32]. A non-invasive method to assess its occurrence could be of paramount importance to improve patient outcome and decrease morbidity in this species.…”

Section: Introductionmentioning

confidence: 99%

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Usefulness and Reliability of the Bispectral Index during Balanced Anesthesia for Neurovascular Surgery in New Zealand White Rabbits

et al. 2023

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Few data about the electroencephalogram and its calculated indices, such as the bispectral index (BIS), have been reported in rabbits. We aimed to evaluate whether a clinically stable anesthesia was mirrored by consistent and stable BIS values and to investigate the effects of modified cerebral blood supply, due to bilateral carotid clamping and re-opening, on BIS values. We also investigated the effects of fentanyl, as an antinociceptive drug, on the BIS. Sixty-eight rabbits undergoing general anesthesia for surgical creation of carotid bifurcation aneurysms were enrolled. The BIS values were recorded at nine selected time points (TPs) during each procedure and before and after fentanyl administration. The BIS values over time were compared with two-way repeated-measures analysis of variance followed by Tukey test, while the Wilcoxon signed rank test was performed to compare values at clamping and re-opening of the carotids as well as before and after fentanyl administration. The BIS values were significantly lower during anesthesia than at the end of anesthesia and at tracheal extubation; no significant differences were found among other TPs. Adequate depth of anesthesia was mirrored by consistent BIS values among rabbits, and alteration of cerebral blood supply did not modify BIS values, except once. Following fentanyl, BIS values did not change in a clinically relevant way.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Usefulness and Reliability of the Bispectral Index during Balanced Anesthesia for Neurovascular Surgery in New Zealand White Rabbits

et al. 2023

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“…Detailed techniques of graft harvesting, graft preparation, and microsurgical creation of the arterial bifurcation have been described elsewhere. 15 Briefly, bifurcation aneurysms were created by end-to-side anastomosis of the right common carotid artery (CCA) to the left CCA, and interposition of an arterial pouch in 36 female New Zealand White rabbits (see online supplemental figure 2 and online supplemental video 1).…”

Section: Bifurcation Aneurysm Model and Surgical Techniquementioning

confidence: 99%

Aspirin treatment prevents inflammation in experimental bifurcation aneurysms in New Zealand White rabbits

Wanderer

Grüter

Strange

et al. 2021

J NeuroIntervent Surg

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BackgroundAneurysm wall degeneration is linked to growth and rupture. To address the effect of aspirin (ASA) on aneurysm formation under various wall conditions, this issue was analyzed in a novel rabbit bifurcation model.MethodsBifurcation aneurysms created in 45 New Zealand White rabbits were randomized to vital (n=15), decellularized (n=13), or elastase-degraded (n=17) wall groups; each group was assigned to a study arm with or without ASA. At follow-up 28 days later, aneurysms were evaluated for patency, growth, and wall inflammation at macroscopic and histological levels.Results36 rabbits survived to follow-up at the end of the trial. None of the aneurysms had ruptured. Patency was visualized in all aneurysms by intraoperative fluorescence angiography and confirmed in 33 (92%) of 36 aneurysms by MRI/MRA. Aneurysm size was significantly increased in the vital (without ASA) and elastase-degraded (with and without ASA) groups. Aneurysm thrombosis was considered complete in three (50%) of six decellularized aneurysms without ASA by MRI/MRA. Locoregional inflammation of the aneurysm complex was significantly reduced in histological analysis among all groups treated with ASA.ConclusionASA intake prevented inflammation of both the periadventitial tissue and aneurysm wall, irrespective of initial wall condition. Although ASA prevented significant growth in aneurysms with vital walls, this preventive effect did not have an important role in elastase-degraded pouches. In possible translation to the clinical situation, ASA might exert a potential preventive effect during early phases of aneurysm formation in patients with healthy vessels but not in those with highly degenerative aneurysm walls.

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“…The current review established the evidence of CPA surgeries and effective manipulation of the FN within the CPA, based on several previous studies ( Table 8 ) and was further confirmed with radiological examinations and anatomical dissections ( Figure 4 , Table 10 ; Maurer and Mika, 1983 ; Lumenta et al, 1988 ; Levine et al, 1993 ; Widick et al, 1994 ; Braun and Richter, 1996 ; Telischi et al, 1999 ; Roethlisberger et al, 2015 ). It is of note that a balanced anesthesiologic approach should be the gold standard in systematically performing experiments in this area, besides an established surgical protocol, to avoid confounders (Wanderer et al, 2020 ). Analgesia should allow for placing a metallic head-post-device firmly attached to the vertex, so that the animal's head can be securely fixed.…”

Section: Future Research Perspectives For Facial Nerve Repair and Regeneration After Cpa-tumor Surgerymentioning

confidence: 99%

Clinical Studies and Pre-clinical Animal Models on Facial Nerve Preservation, Reconstruction, and Regeneration Following Cerebellopontine Angle Tumor Surgery–A Systematic Review and Future Perspectives

Hostettler

Jayashankar

Bikis

et al. 2021

Front. Bioeng. Biotechnol.

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Background and purpose: Tumorous lesions developing in the cerebellopontine angle (CPA) get into close contact with the 1st (cisternal) and 2nd (meatal) intra-arachnoidal portion of the facial nerve (FN). When surgical damage occurs, commonly known reconstruction strategies are often associated with poor functional recovery. This article aims to provide a systematic overview for translational research by establishing the current evidence on available clinical studies and experimental models reporting on intracranial FN injury.Methods: A systematic literature search of several databases (PubMed, EMBASE, Medline) was performed prior to July 2020. Suitable articles were selected based on predefined eligibility criteria following the Preferred Reporting Items for Systematic Reviews and Meta Analyses (PRISMA) guidelines. Included clinical studies were reviewed and categorized according to the pathology and surgical resection strategy, and experimental studies according to the animal. For anatomical study purposes, perfusion-fixed adult New Zealand white rabbits were used for radiological high-resolution imaging and anatomical dissection of the CPA and periotic skull base.Results: One hundred forty four out of 166 included publications were clinical studies reporting on FN outcomes after CPA-tumor surgery in 19,136 patients. During CPA-tumor surgery, the specific vulnerability of the intracranial FN to stretching and compression more likely leads to neurapraxia or axonotmesis than neurotmesis. Severe FN palsy was reported in 7 to 15 % after vestibular schwannoma surgery, and 6% following the resection of CPA-meningioma. Twenty-two papers reported on experimental studies, out of which only 6 specifically used intracranial FN injury in a rodent (n = 4) or non-rodent model (n = 2). Rats and rabbits offer a feasible model for manipulation of the FN in the CPA, the latter was further confirmed in our study covering the radiological and anatomical analysis of perfusion fixed periotic bones.Conclusion: The particular anatomical and physiological features of the intracranial FN warrant a distinguishment of experimental models for intracranial FN injuries. New Zealand White rabbits might be a very cost-effective and valuable option to test new experimental approaches for intracranial FN regeneration. Flexible and bioactive biomaterials, commonly used in skull base surgery, endowed with trophic and topographical functions, should address the specific needs of intracranial FN injuries.

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Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

Cited by 8 publications

References 0 publications

Usefulness and Reliability of the Bispectral Index during Balanced Anesthesia for Neurovascular Surgery in New Zealand White Rabbits

Usefulness and Reliability of the Bispectral Index during Balanced Anesthesia for Neurovascular Surgery in New Zealand White Rabbits

Aspirin treatment prevents inflammation in experimental bifurcation aneurysms in New Zealand White rabbits

Clinical Studies and Pre-clinical Animal Models on Facial Nerve Preservation, Reconstruction, and Regeneration Following Cerebellopontine Angle Tumor Surgery–A Systematic Review and Future Perspectives

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