Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

Bussmann, Jeroen; Bos, Frank L.; Urasaki, Akihiro; Kawakami, Koichi; Duckers, Henricus J.; Schulte‐Merker, Stefan

doi:10.1242/dev.048207

Cited by 201 publications

(242 citation statements)

References 14 publications

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“…These lymphatic vessels include the facial lymphatics (FLs) [also observed previously (Yaniv et al, 2006;Bussmann et al, 2010)] (Fig. 1C, left panels), the lateral lymphatics (LL) and the intestinal lymphatics (IL), which connect to the TD (Okuda et al, 2012) (Fig.…”

Section: Zebrafishmentioning

confidence: 75%

Getting out and about: the emergence and morphogenesis of the vertebrate lymphatic vasculature

et al. 2013

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SummaryThe lymphatic vascular system develops from the pre-existing blood vasculature of the vertebrate embryo. New insights into lymphatic vascular development have recently been achieved with the use of alternative model systems, new molecular tools, novel imaging technologies and growing interest in the role of lymphatic vessels in human disorders. The signals and cellular mechanisms that facilitate the emergence of lymphatic endothelial cells from veins, guide migration through the embryonic environment, mediate interactions with neighbouring tissues and control vessel maturation are beginning to emerge. Here, we review the most recent advances in lymphatic vascular development, with a major focus on mouse and zebrafish model systems.

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Section: Zebrafishmentioning

confidence: 75%

Getting out and about: the emergence and morphogenesis of the vertebrate lymphatic vasculature

et al. 2013

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“…To assess when the first invading vessels express arterial markers, cryoinjury was performed in Tg(kdrl:EGFP) and Tg(-0.8flt1:RFP) lines, reported to show fluorescent protein expression mainly in arteries at early developmental stages (22,23). We first used the caudal fin to test whether these transgenes label arteries in adult animals.…”

Section: Resultsmentioning

confidence: 99%

Fast revascularization of the injured area is essential to support zebrafish heart regeneration

Marín-Juez

Marass

Gauvrit

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

167

222

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Zebrafish have a remarkable capacity to regenerate their heart. Efficient replenishment of lost tissues requires the activation of different cell types including the epicardium and endocardium. A complex set of processes is subsequently needed to support cardiomyocyte repopulation. Previous studies have identified important determinants of heart regeneration; however, to date, how revascularization of the damaged area happens remains unknown. Here, we show that angiogenic sprouting into the injured area starts as early as 15 h after injury. To analyze the role of vegfaa in heart regeneration, we used vegfaa mutants rescued to adulthood by vegfaa mRNA injections at the one-cell stage. Surprisingly, vegfaa mutants develop coronaries and revascularize after injury. As a possible explanation for these observations, we find that vegfaa mutant hearts up-regulate the expression of potentially compensating genes. Therefore, to overcome the lack of a revascularization phenotype in vegfaa mutants, we generated fish expressing inducible dominant negative Vegfaa. These fish displayed minimal revascularization of the damaged area. In the absence of fast angiogenic revascularization, cardiomyocyte proliferation did not occur, and the heart failed to regenerate, retaining a fibrotic scar. Hence, our data show that a fast endothelial invasion allows efficient revascularization of the injured area, which is necessary to support replenishment of new tissue and achieve efficient heart regeneration. These findings revisit the model where neovascularization is considered to happen concomitant with the formation of new muscle. Our work also paves the way for future studies designed to understand the molecular mechanisms that regulate fast revascularization.heart regeneration | angiogenesis | revascularization | coronaries | VEGF

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“…After the parachordal has formed lymphatic progenitors migrate dorsally and ventrally along arterial intersegmental vessels to begin formation of the rest of the trunk lymphatic network (Yaniv et al 2006;Bussmann et al 2010). These processes are highly stereotyped-lymphatic progenitors always migrate along arterial intersegmental vessels but not venous intersegmental vessels, showing that arterial blood vessels act as specific guidance templates for migration of LECs (Yaniv et al 2006;Bussmann et al 2010).…”

Section: Formation Of the Lymphatic Systemmentioning

confidence: 99%

“…These processes are highly stereotyped-lymphatic progenitors always migrate along arterial intersegmental vessels but not venous intersegmental vessels, showing that arterial blood vessels act as specific guidance templates for migration of LECs (Yaniv et al 2006;Bussmann et al 2010). The ventrally-migrating LECs branch rostally and caudally between the dorsal aorta and PCV to form the thoracic duct via proliferation of migrating LECs (Yaniv et al 2006).…”

Section: Formation Of the Lymphatic Systemmentioning

confidence: 99%

Vascular Development in the Zebrafish

Gore¹,

Monzo²,

Young³

et al. 2012

Cold Spring Harbor Perspectives in Medicine

238

166

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The zebrafish has emerged as an excellent vertebrate model system for studying blood and lymphatic vascular development. The small size, external and rapid development, and optical transparency of zebrafish embryos are some of the advantages the zebrafish model system offers. Multiple well-established techniques have been developed for imaging and functionally manipulating vascular tissues in zebrafish embryos, expanding on and amplifying these basic advantages and accelerating use of this model system for studying vascular development. In the past decade, studies performed using zebrafish as a model system have provided many novel insights into vascular development. In this article we discuss the amenability of this model system for studying blood vessel development and review contributions made by this system to our understanding of vascular development.

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Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

Cited by 201 publications

References 14 publications

Getting out and about: the emergence and morphogenesis of the vertebrate lymphatic vasculature

Getting out and about: the emergence and morphogenesis of the vertebrate lymphatic vasculature

Fast revascularization of the injured area is essential to support zebrafish heart regeneration

Vascular Development in the Zebrafish

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