Artery regional properties and atherosclerosis susceptibility

Guo, Feng; Jokinen, Micheal P.

doi:10.1016/j.lfs.2006.09.014

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Because DCN and SPON1 are synthetic proteins, and also respond to TGFβ, it appears that TGFβ may be involved in the phenotypic differences observed between the WC-As and SR-Ar in previous studies. Wagner et al (2007) found that TGFβ decreased proliferation in SR-Ar cells but increased growth in WC-As cells from the comparable site in the celiac region.…”

Section: Theme 3: Cell Signaling Pathwaysmentioning

confidence: 88%

Differentially expressed genes in aortic smooth muscle cells from atherosclerosis-susceptible and atherosclerosis-resistant pigeons

et al. 2012

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Susceptibility to spontaneous atherosclerosis in the White Carneau (WC-As) pigeon shows autosomal recessive inheritance. Aortic smooth muscle cells (SMC) cultured from susceptible WC-As and resistant Show Racer (SR-Ar) pigeons exhibit developmental and degenerative features corresponding to the respective SMC at atherosclerosis-prone sites in vivo. We used representational difference analysis to identify differentially expressed genes between WC-As and SR-Ar aortic SMC. Total RNA was extracted from cultured primary SMC of each breed, converted to double-stranded cDNA, followed by direct comparison in reciprocal representational difference analysis experiments. Difference products were cloned, sequenced, and identified by BLAST against the chicken genome. Six putative biochemical pathways were distinctly different between breeds with genes involved in energy metabolism and contractility exhibiting the most striking disparity. Genes associated with glycolysis and a synthetic SMC phenotype were expressed in WC-As cells. In contrast, SR-Ar cells expressed genes indicative of oxidative phosphorylation and a contractile SMC phenotype. In WC-As cells, the alternatives of insufficient ATP production limiting contractile function or the lack of functional contractile elements downregulating ATP synthesis cannot be distinguished due to the compressed in vitro versus in vivo developmental time frame. However, the genetic potential for effectively coupling energy production to muscle contraction present in the resistant SR-Ar was lacking in the susceptible WC-As.

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Section: Theme 3: Cell Signaling Pathwaysmentioning

confidence: 88%

Differentially expressed genes in aortic smooth muscle cells from atherosclerosis-susceptible and atherosclerosis-resistant pigeons

et al. 2012

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“…Their feral descendants are often disdained in urban environments (Jerolmack, 2008) by the very humans that create ideal cliff-like habitats for them, provide them with plentiful food year-round, and are largely responsible for their nearly worldwide distribution. Yet pigeons have also assumed the roles of sacred religious symbols and war heroes (Hansell, 1998), and they are important model organisms for research in parasitology, behavior, physiology, psychology, neuroscience, microbiology, and other disciplines (Abs, 1983; Blaisdell and Cook, 2005; Fagot and Cook, 2006; Harbison et al, 2009; Klopfleisch et al, 2006; Lauwers et al, 2013; Mehlhorn and Rehkamper, 2009; Ros et al, 2011; Wagner et al, 2007). Here, we review the motivation and recent progress towards understanding the genetic and developmental mechanisms controlling pigeon diversity, including some of the same traits that struck “Darwin’s fancy” (Montgomerie, 2009).…”

Section: Introductionmentioning

confidence: 99%

Pigeonetics takes flight: Evolution, development, and genetics of intraspecific variation

Domyan

Shapiro

2017

Developmental Biology

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Intensive artificial selection over thousands of years has produced hundreds of varieties of domestic pigeon. As Charles Darwin observed, the morphological differences among breeds can rise to the magnitude of variation typically observed among different species. Nevertheless, different pigeon varieties are interfertile, thereby enabling forward genetic and genomic approaches to identify genes that underlie derived traits. Building on classical genetic studies of pigeon variation, recent molecular investigations find a spectrum of coding and regulatory alleles controlling derived traits, including plumage color, feather growth polarity, and limb identity. Developmental and genetic analyses of pigeons are revealing the molecular basis of variation in a classic example of extreme intraspecific diversity, and have the potential to nominate genes that control variation among other birds and vertebrates in general.

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The pigeon (Columba livia) model of spontaneous atherosclerosis

2014

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Multiple animal models have been employed to study human atherosclerosis, the principal cause of mortality in the United States. Each model has individual advantages related to specific pathologies. Initiation, the earliest disease phase, is best modeled by the White Carneau (WC-As) pigeon. Atherosclerosis develops spontaneously in the WC-As without either external manipulation or known risk factors. Furthermore, susceptibility is caused by a single gene defect inherited in an autosomal recessive manner. The Show Racer (SR-Ar) pigeon is resistant to atherosclerosis. Breed differences in the biochemistry and metabolism of celiac foci cells have been described. For example, WC-As have lower oxidative metabolism but higher amounts of chondroitin-6-sulfate and nonesterified fatty acids compared with SR-Ar. Gene expression in aortic smooth muscle cells was compared between breeds using representational difference analysis and microarray analysis. Energy metabolism and cellular phenotype were the chief gene expression differences. Glycolysis and synthetic cell types were related to the WC-As but oxidative metabolism and contractile cell types were related to the SR-Ar. Rosiglitazone, a PPARγ agonist, blocked RNA binding motif (RBMS1) expression in WC-As cells. The drug may act through the c-myc oncogene as RBMS1 is a c-myc target. Proteomic tests of aortic smooth muscle cells supported greater glycosylation in the WC-As and a transforming growth factor β effect in SR-Ar. Unoxidized fatty acids build up in WC-As cells because of their metabolic deficiency, ultimately preventing the contractile phenotype in these cells. The single gene responsible for the disease is likely regulatory in nature.

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Artery regional properties and atherosclerosis susceptibility

Cited by 3 publications

References 20 publications

Differentially expressed genes in aortic smooth muscle cells from atherosclerosis-susceptible and atherosclerosis-resistant pigeons

Differentially expressed genes in aortic smooth muscle cells from atherosclerosis-susceptible and atherosclerosis-resistant pigeons

Pigeonetics takes flight: Evolution, development, and genetics of intraspecific variation

The pigeon (Columba livia) model of spontaneous atherosclerosis

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