Arthritogenic Alphavirus Capsid Protein

Rao, Shambhavi; Taylor, Adam

doi:10.3390/life11030230

Cited by 7 publications

(5 citation statements)

References 107 publications

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“…The diversity of the receptor-binding domain might determine the host tropisms of alphaviruses. The CP provides a physical protection shell for the viral genome RNA and may also be involved in viral genome replication [25].…”

Section: Introductionmentioning

confidence: 99%

Structural Insights into Alphavirus Assembly Revealed by the Cryo-EM Structure of Getah Virus

Wang

Sun

Cui

et al. 2022

Viruses

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Getah virus (GETV) is a member of the alphavirus genus, and it infects a variety of animal species, including horses, pigs, cattle, and foxes. Human infection with this virus has also been reported. The structure of GETV has not yet been determined. In this study, we report the cryo-EM structure of GETV at a resolution of 3.5 Å. This structure reveals conformational polymorphism of the envelope glycoproteins E1 and E2 at icosahedral 3-fold and quasi-3-fold axes, which is believed to be a necessary organization in forming a curvature surface of virions. In our density map, three extra densities are identified, one of which is believed a “pocket factor”; the other two are located by domain D of E2, and they may maintain the stability of E1/E2 heterodimers. We also identify three N-glycosylations at E1 N141, E2 N200, and E2 N262, which might be associated with receptor binding and membrane fusion. The resolving of the structure of GETV provides new insights into the structure and assembly of alphaviruses and lays a basis for studying the differences of biology and pathogenicity between arthritogenic and encephalitic alphaviruses.

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Section: Introductionmentioning

confidence: 99%

Structural Insights into Alphavirus Assembly Revealed by the Cryo-EM Structure of Getah Virus

Wang

Sun

Cui

et al. 2022

Viruses

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“…The multifunctional arthritogenic alphavirus capsid protein is crucial for viral infection and has roles in genome encapsulation, budding, and virion assembly. The arthritogenic alphaviral capsid protein can play an important role in vaccine design, can be regarded as a therapeutic target, and can play an important role in the development of diagnosis ( Rao and Taylor, 2021 ). CHIKV can be genetically divided into three lineages: West African; East, Central, and South African; Indian Ocean; and Asian.…”

Section: Resultsmentioning

confidence: 99%

Research trends on alphavirus receptors: a bibliometric analysis

Chen,

Wang,

Zhang

2024

Front. Cell. Infect. Microbiol.

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BackgroundAlphaviruses are a diverse group of pathogens that have garnered considerable attention due to their impact on human health. By investigating alphavirus receptors, researchers can elucidate viral entry mechanisms and gain important clues for the prevention and treatment of viral diseases. This study presents an in-depth analysis of the research progress made in the field of alphavirus receptors through bibliometric analysis.MethodsThis study encompasses various aspects, including historical development, annual publication trends, author and cited-author analysis, institutional affiliations, global distribution of research contributions, reference analysis with strongest citation bursts, keyword analysis, and a detailed exploration of recent discoveries in alphavirus receptor research.ResultsThe results of this bibliometric analysis highlight key milestones in alphavirus receptor research, demonstrating the progression of knowledge in this field over time. Additionally, the analysis reveals current research hotspots and identifies emerging frontiers, which can guide future investigations and inspire novel therapeutic strategies.ConclusionThis study provides an overview of the state of the art in alphavirus receptor research, consolidating the existing knowledge and paving the way for further advancements. By shedding light on the significant developments and emerging areas of interest, this study serves as a valuable resource for researchers, clinicians, and policymakers engaged in combating alphavirus infections and improving public health.

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“…All the non-structural proteins (nsP1-nsP4) are normally required for RNA synthesis [ 49 ], but the nsP2 protein is established as a virulence factor and inhibitor of the IFN-induced JAK/STAT pathway [ 48 ]. In addition, the essential interactions between the capsid protein and the E1 or E2 protein has also been underlined [ 50 ]. We showed that all the studied compounds efficiently downregulated E1 and E2 gene expression during ONNV infection, in spite of a distinct upregulation of capsid and nsP2 gene expression by MAT.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Analyses of the Multifocal Effects of Natural Alkaloids Berberine, Matrine, and Tabersonine against the O’nyong-nyong Arthritogenic Alphavirus Infection and Inflammation

Seteyen¹,

Guiraud²,

Gasque

et al. 2023

Pharmaceuticals

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O’nyong-nyong virus (ONNV) is a member of the reemerging arthritogenic alphaviruses that cause chronic debilitating polyarthralgia and/or polyarthritis via their tropism for the musculoskeletal system. Thus, the discovery of dual antiviral and anti-inflammatory drugs is a great challenge in this field. We investigated the effects of the common plant-derived alkaloids berberine (isoquinoline), matrine (quinolizidine), and tabersonine (indole) at a non-toxic concentration (10 μM) on a human fibroblast cell line (HS633T) infected by ONNV (MOI 1). Using qRT-PCR analyses, we measured the RNA levels of the gene coding for the viral proteins and for the host cell immune factors. These alkaloids demonstrated multifocal effects by the inhibition of viral replication, as well as the regulation of the type-I interferon antiviral signaling pathway and the inflammatory mediators and pathways. Berberine and tabersonine proved to be the more valuable compounds. The results supported the proposal that these common alkaloids may be useful scaffolds for drug discovery against arthritogenic alphavirus infection.

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Arthritogenic Alphavirus Capsid Protein

Cited by 7 publications

References 107 publications

Structural Insights into Alphavirus Assembly Revealed by the Cryo-EM Structure of Getah Virus

Structural Insights into Alphavirus Assembly Revealed by the Cryo-EM Structure of Getah Virus

Research trends on alphavirus receptors: a bibliometric analysis

In Vitro Analyses of the Multifocal Effects of Natural Alkaloids Berberine, Matrine, and Tabersonine against the O’nyong-nyong Arthritogenic Alphavirus Infection and Inflammation

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