Arthritogenicity of Unaltered and Acetylated Cell Walls of Mycobacteria

Bonhomme, Frédéric; Boucheron, Charlotte; Migliore, D; Jollès, P.

doi:10.1159/000230752

Cited by 13 publications

(9 citation statements)

References 2 publications

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“…hominis and var. bovis (18) have arthritogenic activity. T h e mechanism by which cell walls o f bacteria induce arthritis is not clear at the present time.…”

Section: Discussionmentioning

confidence: 99%

Studies on experimental arthritis induced by Corynebacterium rubrum. 1. Localization of the arthritogenic factor in the cell walls

Paronetto

1972

Arthritis & Rheumatism

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Purified cell walls of Corynebacterium rubrum injected intradermally into rats together with mineral oil induce a severe progressive form of arthritis. The arthritogenic factor contains glucosamine, muramic acid, and several amino acids. It is resistant to digestion with proteolytic enzymes and to extraction with alcoholether, chloroform, and phenol, but is lost after digestion with lysozyme. These findings suggest a link between the cell wall of Corynebacterium rubrummost likely a mucopeptide-and the development of arthritis.Previous investigations have indicated that Corynebacterium rubrum-a grampositive, nonpathogenic bacterium-induces severe arthritis 7 to 10 days after its injection into rats (1). The present study was undertaken to investigate the nature of the arthritogenic factor, and reports that the factor endowed with arthritogenic activity is localized in the cell wall of Corynebacterium rubrum. approximately 150 g were used. Purified cell walls were tested on male Lewis rats weighing 150 g.+ injection of bacteria. Corynebacterium rubrum CR) or its fractions were homogenized in heavy mineral oil at a concentration of 6 mg/ml. Animals were injected with 0.1 ml of the suspension intracutaneously into the base of the tail. MATERIALS AND METHODS AnimalsQuantitation of arthritis. The involvement of the four extremities was graded 0 to 5 depending upon the degree of swelling and redness (1). Final reading was made 30 days after the injection of bacteria.Culture of corynebacterium rubrum. Corynebacterium rubrum? was grown for 7 days at 37°C in I000 ml Erlenmeyer flasks containing approximately 250 ml of brain-heart infusion and 1.5% maltose. The bacteria were then centrifuged, washed 3 times with physiological saline, autoclaved, washed 3 times with distilled water, then lyophilized.Fractionation of corynebacterium rubrum. Fractionation was performed according to the following schema:+Microbiological Associates, Walkersville, Mary+American-type culture collection, Rockville, land.Maryland. 36

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“…hominis and var. bovis (18) have arthritogenic activity. T h e mechanism by which cell walls o f bacteria induce arthritis is not clear at the present time.…”

Section: Discussionmentioning

confidence: 99%

Studies on experimental arthritis induced by Corynebacterium rubrum. 1. Localization of the arthritogenic factor in the cell walls

Paronetto

1972

Arthritis & Rheumatism

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“…Adjuvant-induced arthritis in rats (AA) has been produced by a single injection of water-in-oil emulsion, containing Mycobacterium [20], myco bacterial cell walls [2,4] and wax D [25]. It is also known that heat-killed cells or cell walls of Nocardia [8] and Corynebacterium [19] were able to produce the disease.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Various Fractions from <i>Mycobacterium smegmati</i><i>s</i>, Their Arthritogenicity and Their Preventive Effect on Adjuvant Disease

Kohashi¹,

Pearson²,

Shimono³

et al. 1976

Int Arch Allergy Immunol

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Cell walls of Mycobacterium smegmatis were able to produce much more severe arthritis in rats than the delipidated cells, whereas cell envelope and cell membrane fractions were unable to produce the disease. The lysozyme-solubilized product was able to produce mild disease with only 30% of incidence with an optimum dose, whereas the higher and the lower doses did not produce the disease. The rats immunized with cell envelope, cell membrane fraction and nonarthritogenic doses of lysozyme-solubilized product were protected against the subsequent homologous and heterologous challenge of delipidated cells. It was discussed that this preventative effect can be the result of antigenic competition between the arthritogenic and nonarthritogenic components of M. smegmatis. On the other hand, all the fractions separated here were able to serve as an immunoadjuvant in terms of inducing delayed hypersensitivity to ovalbumin in guinea pigs.

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“…Bonhomme et al [4] found that wax Dp is associated with the arthritogenicity of wax D, while wax Ds is not only nonarthritogenic, but also preventive against a subsequent challenge with wax Dp. A mixture of wax Dp and wax Dr seemed to be less effective for production of arthritis than wax Dp alone [4], Furthermore, they found that a molecu lar ratio of wax Dp and wax Dr is varied among different strains of mycobacterium [6], It is, there fore, conceivable that the arthritogenicity of wax D may be closely associated with the amounts of wax Dp available in wax D in different oil compo sitions. If this is the case, it is possible that the su periority of squalane to mineral oil may be the re sult of the different solubility between wax Dp and wax Dr in both oil vehicles.…”

Section: Discussionmentioning

confidence: 99%

Arthritogenicity of Wax D from Various Mycobacteria Related to Oil Vehicle Composition and to the Combination with Poly I:C, Cord Factor and Acetylated Wax D

Kohasi¹,

Pearson²,

Koga³

1977

Int Arch Allergy Immunol

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Most of wax D (peptidoglycolipid) used here appeared to be ineffective for production of arthritis when given in a water-in-oil emulsion, while the same wax D in squalane was very effective for production of arthritis. Arlacel A as an emulsifier appeared to suppress the arthritogenicity of wax D in squalane, probably through some interaction with the arthritogenic portion of wax D. Poly 1:C seemed to remarkably enhance the arthritogenicity of wax D, even in water-in-oil emulsion. Acetylated wax D and cord factor (trehalose-dimycolate) were much less effective than poly 1:C. Delayed skin hypersensitivity to PPD, peptidoglycan and poly 1:C was also markedly affected by oil composition. However, there was no correlation between these delayed hypersensitivities and development of arthritis.

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Arthritogenicity of Unaltered and Acetylated Cell Walls of Mycobacteria

Cited by 13 publications

References 2 publications

Studies on experimental arthritis induced by Corynebacterium rubrum. 1. Localization of the arthritogenic factor in the cell walls

Studies on experimental arthritis induced by Corynebacterium rubrum. 1. Localization of the arthritogenic factor in the cell walls

Preparation of Various Fractions from <i>Mycobacterium smegmati</i><i>s</i>, Their Arthritogenicity and Their Preventive Effect on Adjuvant Disease

Arthritogenicity of Wax D from Various Mycobacteria Related to Oil Vehicle Composition and to the Combination with Poly I:C, Cord Factor and Acetylated Wax D

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