Arthroplasty Using Autologous Multipotent Mesenchymal Cells and Collagen Membrane Chondro-Gide

Bryanskiy, A. I; Kulyaba, T.A.; Kornilov, N.N.; Румакин, В. П.

doi:10.32414/0869-8678-2014-1-62-66

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Еще в 1783 г. было отмечено, что, будучи однажды поврежденным, такой хрящ не способен к регенерации [9][10][11]. В настоящее время большое количество работ посвящены разработке новых перспективных методов восстановления гиалинового хряща [12][13][14]. Основной задачей при этом является обеспечение оптимальных условий для регенерации или репарации хрящевой ткани [15,16].…”

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“…Применение таких клеточно-инженерных конструкций (КИК) показывает хорошие результаты, но лишь в среднесрочной перспективе [28,29,30]. Однако при длительном наблюдении и они также проявляют признаки деградирования [14,25]. В настоящее время одним из наиболее отработанных подходов является белковая модификация клеточной культуры цитокином Tgfβ3, играющим ключевую роль в хондрогенной пролиферации клеточной культуры.…”

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Results of the Use Tgfβ3 for Modify Cell Engineering Construct Recovery the Defect of the Articular Hyaline Cartilage

Bozhokin¹,

Божкова²,

Нетылько³

et al. 2020

СПНО (MPSE)

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Восстановление гиалинового слоя суставной поверхности является важной и актуальной задачей медицины, которая полностью не решена до настоящего времени. Одним из перспективных направлений ее решения является трансплантация специализированной клеточно-инженерной конструкции (КИК) из биодеградируемого полимера (скаффолда) и культуры аутологичных клеток. Цель исследования: оценить влияние цитокина Tgfβ3 на экспрессию генов хондрогенеза (col2a1, tgfβ3, sox9) и динамику перифокальных реакций при замещении дефекта суставного хряща клеточноинженерной конструкцией в эксперименте. Методом РВ-ПЦР в эксперименте in vitro была выполнена оценка экспрессии генов col2a1, tgfβ3, sox9 в культуре ММСК при воздействии цитокина Tgfβ3. В эксперименте in vivo на 27 половозрелых крысах изучали динамику гистологических изменений на 14-е, 30-е и 90-е сутки после формирования дефекта суставного хряща в 3 группах экспериментальных животных: без замещения дефекта, с трансплантацией клеточно-инженерной конструкции с культурой мезенхимных мультипотентных стромальных клеток (ММСК) без модификации и с модификацией цитокином Tgfβ3. Установлено относительное увеличение экспрессии генов col2a1, Tgfβ3, sox9 в 800, 20 и 120 раз соответственно под действием цитокина Tgfβ3 на культуру ММСК in vitro. Ключевые слова: ММСК, гиалиновый хрящ, Tgfβ3, клеточно-инженерная конструкция, дефект.

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Results of the Use Tgfβ3 for Modify Cell Engineering Construct Recovery the Defect of the Articular Hyaline Cartilage

Bozhokin¹,

Божкова²,

Нетылько³

et al. 2020

СПНО (MPSE)

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The Effectiveness of Various Surgical Techniques in the Treatment of Local Knee Cartilage Lesions (Review)

Куляба

Bantser

Trachuk

et al. 2020

Traumatology and Orthopedics of Russia

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Introduction. To restore the knee local cartilage lesions, a large number of alternative surgical techniques are used in clinical practice: isolated debridement of the lesion area, chondrogenesis stimulation, mosaic osteochondral grafting, cell technologies, collagen membranes (matrices), and a combination of the above methods. The purpose of this article was to compare the effectiveness of various surgical methods of treating patients with local cartilage lesions of the femur based on analysis of relevant publications. Materials and Methods. The review included 85 publications of domestic and foreign authors within 2005 to 2020. The search was carried out in electronic scientific databases PubMed and eLIBRARy. Results. The medium and long term outcomes of debridement and/or various options of chondrogenesis stimulating, despite their wide popularity, in terms of clinical, radiological, and histological indicators, are inferior to all other surgical techniques. Mosaic osteochondral auto-and/or allografting, as well as transplantation of autologous chondrocytes culture with a collagen membrane, are characterized by the best 15 to 20-year outcomes, allowing most patients to maintain the same level of activity as before the lesion occurred. The combination of matrices with other cellular products or microfracturing shows similar medium-term results, but it long-term efficacy remains unknown. Conclusion. The use of debridement and/or chondrogenesis stimulation should be limited to minimal defects. From both a clinical and an economic point of view, mosaic osteochondral grafting is the optimal method for the treatment of knee local cartilage lesions with an area up to 4 to 6 cm 2 . The combination of membranes with various cellular products or microfracturing is indicated in case of extensive local cartilage lesions or if mosaic osteochondral grafting is not appropriate.

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Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

et al. 2021

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The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

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Arthroplasty Using Autologous Multipotent Mesenchymal Cells and Collagen Membrane Chondro-Gide

Cited by 3 publications

References 5 publications

Results of the Use Tgfβ3 for Modify Cell Engineering Construct Recovery the Defect of the Articular Hyaline Cartilage

Results of the Use Tgfβ3 for Modify Cell Engineering Construct Recovery the Defect of the Articular Hyaline Cartilage

The Effectiveness of Various Surgical Techniques in the Treatment of Local Knee Cartilage Lesions (Review)

Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

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