Arthroscopic debridement and bone marrow stimulation for talar osteochondral lesions: current concepts

Canata, Gian Luigi; Casale, Valentina

doi:10.1136/jisakos-2016-000099

Cited by 3 publications

(2 citation statements)

References 48 publications

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“…1 Because cartilage tissue exhibits lack of vascularization and the metabolic activity of mature chondrocytes is limited, selfrepair is difficult after injury. 2 The current existing clinical treatments for osteochondral defect, including arthroscopic debridement, 3 artificial joint replacement, 4 chondral and osteochondral grafts, [5][6][7] and bone marrow stimulation, [8][9][10] have disadvantages such as donor-site morbidity, anatomical mismatching, pain, and infection. 5 In recent years, tissue-engineered scaffolds have been widely utilized to treat the osteochondral defects.…”

Section: Introductionmentioning

confidence: 99%

Bi-layered Composite Scaffold for Repair of the Osteochondral Defects

Cheng

Dai

et al. 2021

Advances in Wound Care

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Objective: Osteochondral defect presents a big challenge for clinical treatment. This study aimed at constructing a bi-layered composite chitosan/ chitosan-b-tricalcium phosphate (CS/CS-b-TCP) scaffold and at repairing the rat osteochondral defect. Approach: The bi-layered CS/CS-b-TCP scaffold was fabricated by lyophilization, and its microstructure was observed by a scanning electron microscope. Chondrocytes and bone marrow stem cells (BMSCs) were seeded into the CS layer and the CS-b-TCP layer, respectively. Viability and proliferation ability of the cells were observed under a confocal microscope. After subcutaneous implantation, the chondrogenic ability of the CS layer and osteogenic ability of the CS-b-TCP layer were evaluated by immunofluorescence. Then, the bilayered scaffolds were implanted into the rat osteochondral defects and the harvested samples were macroscopically and histologically evaluated. Results: The bi-layered CS/CS-b-TCP scaffold exhibited the distinctive microstructures for each layer. The seeded chondrocytes in the CS layer could maintain the chondrogenic lineage, whereas BMSCs in the CS-b-TCP layer could continually differentiate into the osteogenic lineage. Moreover, cells in both layers could maintain well viability and excellent proliferation ability. For the in vivo study, the newly formed tissues in the bi-layered scaffolds group were similar with the native osteochondral tissues, which comprised hyaline-like cartilage and subchondral bone, with better repair effects compared with those of the pure CS group and the blank control group. Innovation: This is the first time that the bi-layered composite CS/CS-b-TCP scaffold has been fabricated and evaluated with respect to osteochondral defect repair. Conclusion: The bi-layered CS/CS-b-TCP scaffolds could facilitate osteochondral defect repair and might be the promising candidates for osteochondral tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Bi-layered Composite Scaffold for Repair of the Osteochondral Defects

Cheng

Dai

et al. 2021

Advances in Wound Care

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“…[1] In the absence of treatment, cartilage damage may initiate degenerative processes in the adjacent cartilage tissue and subchondral bone, which may eventually result in OA. [2] Treatments for chondral damage include arthroscopic debridement, [3] artificial joint replacement, [4] autologous chondrocyte transplantation, [5] chondral and osteochondral grafts, [6] and bone marrow stimulation. [7] The main Objectives: In this study, we aimed to determine the bioefficacy of epidermal growth factor (EGF), boric acid (BA), and their combination on cartilage injury in rats.…”

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confidence: 99%

Investigation of the efficacy of epidermal growth factor, boric acid and their combination in cartilage injury in rats: An experimental study

Kağan Yılmaz,

Nuri Konya,

İnce

et al. 2023

Jt Dis Relat Surg

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Joint cartilage injury brought on by osteoarthritis (OA) or traumatic injuries can result in joint deformity, motion restriction, and severe pain. Due to its avascular nature and restricted metabolic activity of mature chondrocytes, cartilage tissue is unable to self-repair after injury. [1] In the absence of treatment, cartilage damage may initiate degenerative processes in the adjacent cartilage tissue and subchondral bone, which may eventually result in OA. [2] Treatments for chondral damage include arthroscopic debridement, [3] artificial joint replacement, [4] autologous chondrocyte transplantation, [5] chondral and osteochondral grafts, [6] and bone marrow stimulation. [7] The main Objectives: In this study, we aimed to determine the bioefficacy of epidermal growth factor (EGF), boric acid (BA), and their combination on cartilage injury in rats. Materials and methods:In in vitro setting, the cytotoxic effects of BA, EGF, and their combinations using mouse fibroblast cell (L929), human bone osteosarcoma cell (Saos-2), and human adipose derived mesenchymal stem cells (hAD-MSCs) were determined by applying MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] test. In in vivo setting, 72 rats were randomly divided into four groups. A standard chondral defect was created and microfracture was performed in all groups. Group A was determined as the control group. In addition to the standard procedure, Group B received 100 ng/mL of EGF, Group C received a combination of 100 ng/mL of EGF and 10 µg/mL of BA combination, and Group D 20 µg/mL of BA. Results:The cytotoxic effect of the combinations of EGF dilutions (1,5,10,25, 50, 100, 200 ng/mL) with BA (100, 300, 500 µg/mL) was observed only in the 72-h application period and in Saos-2. The cytotoxic effect of BA was reduced when combined with EGF. There was no significant difference in the histopathological scores among the groups (p=0.13). Conclusion:Our study showed that EGF and low-dose BA application had a positive effect on cartilage healing in rats. Significant decreases in recovery scores were observed in the other groups. The combination of EGF and BA promoted osteoblast growth. Detection of lytic lesions in the group treated with 20 µg/mL of BA indicates that BA may have a cytotoxic effect.

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