Arthroscopic Treatment of Cartilage Lesions With Microfracture and BST-CarGel

Steinwachs, Matthias; Waibl, Bernhard; Mumme, Marcus

doi:10.1016/j.eats.2014.02.011

Cited by 41 publications

(23 citation statements)

References 6 publications

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“…The healing capability of this technique is based on the formation of a blood clot and migration of cells from the bone marrow. The functional long term outcome after microfracture surgery has presented variable results, the main shortcomings including limited hyaline repair tissue, variable repair cartilage volume, and possibility of progressive ossification of the regenerated tissue [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells

Hopper

Wardale

Howard

et al. 2015

Stem Cells International

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A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSCs) derived from the infrapatellar fat pad of human OA knee. Cell migration was measured using an xCELLigence electronic migration chamber system in combination with scratch assays. Gene expression was quantified with stem cell PCR arrays and validated using quantitative real-time PCR (rtPCR). In both migration assays PBMCs increased MSC migration by comparison to control. In scratch assay the wound closure was 55% higher after 3 hours in the PBMC stimulated test group (P = 0.002), migration rate was 9 times faster (P = 0.008), and total MSC migration was 25 times higher after 24 hours (P = 0.014). Analysis of MSCs by PCR array demonstrated that PBMCs induced the upregulation of genes associated with chondrogenic differentiation over 15-fold. In conclusion, PBMCs increase both MSC migration and differentiation suggesting that they are an ideal candidate for inclusion in regenerative medicine therapies aimed at cartilage repair.

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Section: Introductionmentioning

confidence: 99%

Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells

Hopper

Wardale

Howard

et al. 2015

Stem Cells International

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“…Minimally invasive arthroscopy does not require surgical hip dislocation with associated risks and morbidity. 13 Reduced stiffness and improved function are achieved with BST-CarGel compared with microfracture alone.…”

Section: Discussionmentioning

confidence: 99%

Labral Gracilis Tendon Allograft Reconstruction and Cartilage Regeneration Scaffold for an Uncontained Acetabular Cartilage Defect of the Hip

MacInnis

Hussain

Coady

et al. 2017

Arthroscopy Techniques

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Hip cartilage injuries are very common, with rates as high as 50% having been reported in some series; abnormal femoral acetabular contact can result in a full-thickness cartilage defect or labral lesion. The prevalence of labral lesions can be as high as 55%. This Technical Note describes an arthroscopic technique to reconstruct an uncontained, fullthickness, focal cartilage defect of the acetabulum, with reconstruction of the missing labrum using a gracilis allograft and use of a biological liquid scaffold for cartilage reconstruction. Capsulotomy, acetabuloplasty, and microfracture with marrow bleeding should be performed simultaneously with the gracilis allograft preparation. The graft is inserted and anchored to reconstruct the missing labrum and to re-create a contained defect. Suction and drying of the joint surfaces are performed while the mixture of BST-CarGel (Piramal Healthcare, Laval, Quebec, Canada) and blood is prepared. A drop-by-drop technique is then used to reconstruct the cartilage defect.

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“…However, the condition of the microenvironment of cartilage tissue in vivo is somewhat similar 29 therefore, the use of spheroids to create 'artificial' cartilage tissue may be a favorable and relevant model for investigations of in vivo cartilage repair. In cartilage tissue engineering, most of the publications have showed that natural or synthetic polymeric materials, or a combination of the two, are typically used as scaffolds for cell adhesion [30][31][32] . Moreover, several studies have used scaffold-free approaches 33,34 .…”

Section: Introductionmentioning

confidence: 99%

Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells

et al. 2020

Biomed. Res. Ther.

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Introduction: Cartilage damage is one of the injuries that is difficult for the human body to self-repair due to the avascular and completely mature tissue with only few stem or progenitor cells present. Recently, some studies showed that engineered cartilage tissues could be used to treat or improve this injury. This study aimed to produce the cartilage microtissues from the differentiation of scaffold-free spheroids composed of human adipose-derived stem cells. Methods: Human adipose-derived stem cells (ADSCs) were isolated and expanded following the previously published study. They were then cultured in the non-adherent condition to produce spheroids. The spheroids of the ADSCs were collected and induced into cartilage microtissues in the inducible medium for 21 days. The cartilage microtissue was characterized by some cartilage phenotype markers, including the accumulation of extracellular matrix proteins (aggrecan, glycosaminoglycan, and type II collagen), and the expression of certain genes specific to chondrocytes (Sox9, Col2, Col1, and Acan). Results: The results showed that the expression of chondrocyte-specific genes gradually increased during the 21 days of culture for differentiation. On day 21, the microtissues expressed aggrecan, glycosaminoglycan, and type II collagen proteins. Conclusion: This study demonstrated that cartilage microtissues could easily be produced from scaffold-free spheroids from ADSCs. Thus, cartilage microtissues can be produced in this manner for in vivo transplantation to promote cartilage regeneration, or to produce cartilage tissues for in vitro studies.

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Arthroscopic Treatment of Cartilage Lesions With Microfracture and BST-CarGel

Cited by 41 publications

References 6 publications

Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells

Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells

Labral Gracilis Tendon Allograft Reconstruction and Cartilage Regeneration Scaffold for an Uncontained Acetabular Cartilage Defect of the Hip

Method for in vitro production of cartilage microtissues from scaffold-free spheroids composed of human adipose-derived stem cells

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