Articular cartilage calcification in osteoarthritis: Insights into crystal‐induced stress

Ea, Hang‐Korng; Nguyen, Christelle; Bazin, Dominique; Bianchi, Arnaud; Guicheux, Jérôme; Reboul, Pascal; Daudon, Michel; Lioté, Frédéric

doi:10.1002/art.27761

Cited by 135 publications

(115 citation statements)

References 70 publications

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“…Immunohistochemical analyses demonstrated an increase of type I collagen at 3, 6, and 9 months of age in the experimental groups, and showed progressive ossification of the cartilage tissue in a vertical orientation to the surface of the condyle. These findings closely align with another report that OA is associated with accelerating endochondral ossification 30) . Wnt/ -catenin plays an important role in the articular chondrocyte function, as well as in cartilage development at the pre-hypertrophic zone 31,32) .…”

Section: Discussionsupporting

confidence: 93%

TMJ Degenerative Changes in SAMP3 Mice by Occlusal Disharmony and Aging

Elshawi

Wakamatsu

Iinuma

et al. 2012

J. Hard Tissue Biology.

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Aging and mechanical loading by occlusal disharmony are critical and etiological factors for the onset of temporomandibular joint disorders (TMDs). Senescence-accelerated mouse prone 3 (SAMP3) is one of the animal models for studying age-related degenerative changes of various tissues and organs. To evaluate the influences of both the aging and/or the mechanical loading induced by occlusal disharmony on the SAMP3 TMJ condyle, histological and immunohistochemical analyses were performed.Fifty-five, 4-week-old SAMP3 mice were divided into two groups as an experimental and control. In the experimental group, the maxillary molars were trimmed out from the occlusal plane, which also served as mechanical force loading on the condylar surface. Then the condyles were removed and proceeded for histological and immunohistochemical analysis. The subchondral bone ossification was also evaluated by micro-focusing computed tomography (micro-CT) analysis.The results indicated that induced occlusal disharmony in an aging background promotes TMD, including osteoarthritis (OA) like disease. OA-like TMJ lesions in the SAMP3 mice, accompanied by mandibular condylar cartilage degradation, were characterized by loss of proteoglycans (PGs) and changes of the localization of the extracellular matrix, such as collagen type I, II and MMP13. Changes in the microarchitecture of the condyle were also caused under OA-like lesion. Disrupted Wnt/ -catenin signaling by means of immunohistochemical analysis of -catenin contributes to this irreversible cartilage tissue damage.The present study indicates that the experimentally induced occlusal disharmony (under the age-related background) promotes the TMJ OA accompanied by the alteration to components of the extracellular matrix, and Wnt/ -catenin signaling disorder contributes to these irreversible cartilage tissue damage.

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Section: Discussionsupporting

confidence: 93%

TMJ Degenerative Changes in SAMP3 Mice by Occlusal Disharmony and Aging

Elshawi

Wakamatsu

Iinuma

et al. 2012

J. Hard Tissue Biology.

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“…Sharp transitions of proteoglycans and collagen content seen near the edges of the mineralized cartilage may be indicative of an ongoing endochondral ossification-like process. Factors that may have contributed to cartilage calcification are chondrocyte hypertrophy and apoptosis, and the presence of inflammatory cytokines and rh-BMP-7 [40]. Bone morphogenic protein-7 is well known for its ability to stimulate bone formation in vivo [41], but robust bone formation and long-term survival are also dependent on successful angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Multiphasic construct studied in an ectopic osteochondral defect model

Jeon

Vaquette

Theodoropoulos

et al. 2014

J. R. Soc. Interface.

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In vivo osteochondral defect models predominantly consist of small animals, such as rabbits. Although they have an advantage of low cost and manageability, their joints are smaller and more easily healed compared with larger animals or humans. We hypothesized that osteochondral cores from large animals can be implanted subcutaneously in rats to create an ectopic osteochondral defect model for routine and high-throughput screening of multiphasic scaffold designs and/or tissue-engineered constructs (TECs). Bovine osteochondral plugs with 4 mm diameter osteochondral defect were fitted with novel multiphasic osteochondral grafts composed of chondrocyteseeded alginate gels and osteoblast-seeded polycaprolactone scaffolds, prior to being implanted in rats subcutaneously with bone morphogenic protein-7. After 12 weeks of in vivo implantation, histological and micro-computed tomography analyses demonstrated that TECs are susceptible to mineralization. Additionally, there was limited bone formation in the scaffold. These results suggest that the current model requires optimization to facilitate robust bone regeneration and vascular infiltration into the defect site. Taken together, this study provides a proof-of-concept for a high-throughput osteochondral defect model. With further optimization, the presented hybrid in vivo model may address the growing need for a cost-effective way to screen osteochondral repair strategies before moving to large animal preclinical trials.

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“…Previous studies have considered CaCs as a result of joint damage or degeneration [3] and OA progression [4]. On the other hand, other studies have supported the hypothesis that CaCs cause the release of inflammatory cytokines and metalloproteases, inducing Boxidative stress^and accelerating cartilage deterioration [5]. Hence, it remains unclear whether chondrocalcinosis predisposes or results from OA [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Chondrocalcinosis and Associated Knee Joint Degeneration Using MR Imaging: Data from the Osteoarthritis Initiative

et al. 2016

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Objectives To evaluate the ability of different MRI sequences to detect chondrocalcinosis within knee cartilage and menisci, and to analyze the association with joint degeneration. Methods Subjects with radiographic knee chondrocalcinosis (n = 90, age 67.7 ± 7.3 years, 50 women) were selected from the Osteoarthritis Initiative and matched to controls without radiographic chondrocalcinosis (n = 90). Visualization of calcium-containing crystals (CaC) was compared between 3D T1-weighted gradient-echo (T1GE), 3D dual echo steady-state (DESS), 2D intermediate-weighted (IW), and proton density (PD)-weighted fast spin-echo (FSE) sequences obtained with 3T MRI and correlated with a semiquantitative CaC score obtained from radiographs. Structural abnormalities were assessed using Whole-Organ MRI Score (WORMS) and logistic regression models were used to compare cartilage compartments with and without CaC. Results Correlations between CaC counts of MRI sequences and degree of radiographic calcifications were highest for GE (r T1GE = 0.73, P < 0.001; r DESS = 0.68, P < 0.001) compared to other sequences (P > 0.05). Meniscus WORMS was significantly higher in subjects with chondrocalcinosis compared to controls (P = 0.005). Cartilage defects were significantly more frequent in compartments with CaC than without (patella: P = 0.006; lateral tibia: P < 0.001; lateral femur condyle: P = 0.017). Conclusions Gradient-echo sequences were most useful for the detection of chondrocalcinosis and presence of CaC was associated with higher prevalence of cartilage and meniscal damage.

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Articular cartilage calcification in osteoarthritis: Insights into crystal‐induced stress

Cited by 135 publications

References 70 publications

TMJ Degenerative Changes in SAMP3 Mice by Occlusal Disharmony and Aging

TMJ Degenerative Changes in SAMP3 Mice by Occlusal Disharmony and Aging

Multiphasic construct studied in an ectopic osteochondral defect model

Evaluation of Chondrocalcinosis and Associated Knee Joint Degeneration Using MR Imaging: Data from the Osteoarthritis Initiative

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