Articular Cartilage Imaging by the Use of Phase-Contrast Tomography in a Collagen-Induced Arthritis Mouse Model

Lee, Yeom Sik; Heo, Eun-A.; Jun, Hong Young; Kang, Sung Hoon; Kim, Hun Soo; Lee, Myung Soo; Byun, Seung-Jae; Lee, Sang Ho; Park, Seong Hoon; Yoon, Kwon‐Ha

doi:10.1016/j.acra.2009.09.015

Cited by 16 publications

(11 citation statements)

References 29 publications

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“…Different analysis protocols and landmarks will still need to be defined when analyzing new types of tissue. Until now, when applied in low‐resolution mode without tissue staining, phase‐contrast micro‐CT has served much like standard micro‐CT for evaluating bone remodeling in joint diseases (7). When combined with ionized contrast staining such as Hexabrix (8), phase‐contrast micro‐CT may provide sufficient resolution to assess proteoglycan loss in cartilage in mouse OA models, which would be a future application for this approach in the context of OA modeling.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative imaging of murine osteoarthritic cartilage by phase‐contrast micro–computed tomography

Ruan¹,

Dawson²,

Jiang³

et al. 2013

Arthritis & Rheumatism

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Objective Mouse is an optimal model organism in which gene-environment interactions can be studied for the pathogenesis of osteoarthritis. The gold standard in arthritis research in mice is based on histopathology and immunohistochemistry, which are labor intensive, prone to sampling bias and technical variability, and limited in throughput. This study aims to develop a new technique that assesses mouse cartilage by integrating quantitative volumetric imaging techniques. Methods A novel mouse model of osteoarthritis was generated by cruciate ligament transection (CLT) and evaluated by histopathology and immunohistochemistry. Knee joint samples were then imaged by a novel technique that combines high resolution μCT and phase-contrast optics followed by quantitative analyses. A comparative analysis was also performed on a previously established mouse model of osteoarthritis generated by destabilization of medial meniscus (DMM). Results Phase contrast μCT achieved cellular resolution of chondrocytes and quantitative assessment of parameters such as articular cartilage volume and surface area. In mouse models of OA generated by either CLT or DMM, we showed that phase contrast μCT distinguished control and OA cartilage by providing quantitative measures with high reproducibility and minimal variability. Features of OA at the cellular or tissue levels could also be observed in images generated by phase contrast μCT. Conclusion We established an imaging technology that comprehensively assessed and quantified the 2 and 3 dimensional changes of articular cartilage. Application of this technology will facilitate the rapid and high throughput assessment of genetic and therapeutic models of OA in mice.

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Section: Discussionmentioning

confidence: 99%

“…High‐resolution micro–computed tomography (micro‐CT) imaging has been established as the gold standard for assessing the morphology of calcified tissue, such as trabecular and cortical bone in small animals, at high resolution (4–7). However, this approach does not effectively image uncalcified soft tissue such as cartilage.…”

mentioning

confidence: 99%

Quantitative imaging of murine osteoarthritic cartilage by phase‐contrast micro–computed tomography

Ruan¹,

Dawson²,

Jiang³

et al. 2013

Arthritis & Rheumatism

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“…MicroCT has been extensively used to image mineralized animal tissues such as teeth, cartilage and bone (Burghardt et al, 2010;Lee et al, 2010). The high electron density of bones and teeth strongly contrasts with the low electron density of the surrounding soft tissues.…”

Section: Introductionmentioning

confidence: 99%

Three dimensional quantification of biological samples using micro-computer aided tomography (microCT)

Lilje

Marano

et al. 2013

Journal of Microbiological Methods

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“…To date, this method has been successfully utilised to visualise human articular cartilage [20] and detect damage to mouse articular cartilage [21], [22]. Furthermore, a few researchers have applied this technique to assess and quantify changes in the articular cartilage of mice [12].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Assessment of Murine Articular Cartilage and Bone Using X-Ray Phase-Contrast Imaging

Yuan

et al. 2014

PLoS ONE

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Murine models for rheumatoid arthritis (RA) research can provide important insights for understanding RA pathogenesis and evaluating the efficacy of novel treatments. However, simultaneously imaging both murine articular cartilage and subchondral bone using conventional techniques is challenging because of low spatial resolution and poor soft tissue contrast. X-ray phase-contrast imaging (XPCI) is a new technique that offers high spatial resolution for the visualisation of cartilage and skeletal tissues. The purpose of this study was to utilise XPCI to observe articular cartilage and subchondral bone in a collagen-induced arthritis (CIA) murine model and quantitatively assess changes in the joint microstructure. XPCI was performed on the two treatment groups (the control group and CIA group, n = 9 per group) to monitor the progression of damage to the femur from the knee joint in a longitudinal study (at 0, 4 and 8 weeks after primary injection). For quantitative assessment, morphologic parameters were measured in three-dimensional (3D) images using appropriate image analysis software. Our results showed that the average femoral cartilage volume, surface area and thickness were significantly decreased (P<0.05) in the CIA group compared to the control group. Meanwhile, these decreases were accompanied by obvious destruction of the surface of subchondral bone and a loss of trabecular bone in the CIA group. This study confirms that XPCI technology has the ability to qualitatively and quantitatively evaluate microstructural changes in mouse joints. This technique has the potential to become a routine analysis method for accurately monitoring joint damage and comprehensively assessing treatment efficacy.

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Articular Cartilage Imaging by the Use of Phase-Contrast Tomography in a Collagen-Induced Arthritis Mouse Model

Cited by 16 publications

References 29 publications

Quantitative imaging of murine osteoarthritic cartilage by phase‐contrast micro–computed tomography

Quantitative imaging of murine osteoarthritic cartilage by phase‐contrast micro–computed tomography

Three dimensional quantification of biological samples using micro-computer aided tomography (microCT)

Quantitative Assessment of Murine Articular Cartilage and Bone Using X-Ray Phase-Contrast Imaging

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