Articular Cartilage Regeneration in Veterinary Medicine

Voga, Metka; Majdič, Gregor

doi:10.1007/5584_2022_717

Cited by 5 publications

(3 citation statements)

References 272 publications

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“…Mesenchymal stem cells can self-renew ( Romero et al, 2021;Voga et al, 2022) while maintaining their multipotency, as described in numerous scientific publications (Dias et al, 2019). It allows obtaining the required number of cells for further clinical use, in particular, for the treatment of various diseases (Shah et al, 2018;Arnhold et al, 2019).…”

Section: Literature Reviewmentioning

confidence: 95%

“…Currently, mesenchymal stem cells are the most promising type of stem cells for therapeutic applications due to the ease of their isolation and cultivation, and the absence of ethical issues associated with their further use. They are becoming increasingly well-known in veterinary medicine due to their exceptional immunomodulatory properties (De Lima Santos et al, 2019;Grogan et al, 2022;Voga & Majdic, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…The scientific literature is increasingly discussing, analysing and summarising the results of studies designed to apply allogeneic mesenchymal stem cells (MSCs) as a regenerative therapy in veterinary practice, both in experimental animals and in clinical cases (Dias et al, 2019; Grogan et al, 2022;Voga et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Untitled

2023

Ukrainian journal of veterinary sciences

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Section: Literature Reviewmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Untitled

2023

Ukrainian journal of veterinary sciences

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Characterization of the Proteins Secreted by Equine Muscle-Derived Mesenchymal Stem Cells Exposed to Cartilage Explants in Osteoarthritis Model

Dechêne

Colin

Demazy

et al. 2022

Stem Cell Rev and Rep

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Background Osteoarthritis (OA) is a highly prevalent joint degenerative disease for which therapeutic treatments are limited or invasive. Cell therapy based on mesenchymal stem/stromal cells (MSCs) is therefore seen as a promising approach for this disease, in both human and horses. As the regenerative potential of MSCs is mainly conferred by paracrine function, the goal of this study was to characterize the secreted proteins of muscle-derived MSCs (mdMSCs) in an in vitro model of OA to evaluate the putative clinical interest of mdMSCs as cell therapy for joint diseases like osteoarthritis. Methods An equine osteoarthritis model composed of cartilage explants exposed to pro-inflammatory cytokines was first developed. Then, the effects of mdMSC co-culture on cartilage explant were studied by measuring the glycosaminoglycan release and the NO2− production. To identify the underlying molecular actors, stable isotope-labeling by amino acids in cell culture based secreted protein analyses were conducted, in the presence of serum. The relative abundance of highly sequenced proteins was finally confirmed by western blot. Results Co-culture with muscle-derived MSCs decreases the cytokine-induced glycosaminoglycan release by cartilage explants, suggesting a protecting effect of mdMSCs. Among the 52 equine proteins sequenced in the co-culture conditioned medium, the abundance of decorin and matrix metalloproteinase 3 was significantly modified, as confirmed by western blot analyses. Conclusions These results suggest that muscle-derived MSCs could reduce the catabolic effect of TNFα and IL-1β on cartilage explant by decreasing the secretion and activity of matrix metalloproteinase 3 and increasing the decorin secretion. Graphical abstract mdMSCs capacity to reduce the catabolic consequences of cartilage exposure to pro-inflammatory cytokines. These effects can be explained by mdMSC-secreted bioactive such as TIMP-1 and decorin, known as an inhibitor of MMP3 and an anti-inflammatory protein, respectively.

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