Artifacts in magnetic force microscopy of histological sections

Walsh, K. J.; Shiflett, Owen; Shah, Stavan; Renner, Theodore; Soulas, Nicholas; Scharre, Douglas W.; McTigue, Dana M.; Agarwal, Gunjan

doi:10.1016/j.jmmm.2022.170116

Cited by 4 publications

(5 citation statements)

References 35 publications

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“… 64 However, these methods probe much faster dynamic processes, such as confined spin waves and spin-torque transfer, that occur in the GHz regime rather than the sub-MHz magnetization reversal processes of the MNPs described here. While biological magnetic force microscopy has been used to image MNPs in both cell cultures 65 , 66 and tissue samples, 67 the depth sensitivity of this method is limited, and to date only static magnetic field measurements have been performed. Thus, existing magnetic microscopy techniques are not well suited to analyze the magnetization dynamics of biologically relevant MNPs.…”

Section: Discussionmentioning

confidence: 99%

Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples

Sharifabad,

Soucaille,

Wang

et al. 2024

ACS Nano

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The study of exogenous and endogenous nanoscale magnetic material in biology is important for developing biomedical nanotechnology as well as for understanding fundamental biological processes such as iron metabolism and biomineralization. Here, we exploit the magneto-optical Faraday effect to probe intracellular magnetic properties and perform magnetic imaging, revealing the location-specific magnetization dynamics of exogenous magnetic nanoparticles within cells. The opportunities enabled by this method are shown in the context of magnetic hyperthermia; an effect where local heating is generated in magnetic nanoparticles exposed to high-frequency AC magnetic fields. Magnetic hyperthermia has the potential to be used as a cellular-level thermotherapy for cancer, as well as for other biomedical applications that target heat-sensitive cellular function. However, previous experiments have suggested that the cellular environment modifies the magnetization dynamics of nanoparticles, thus dramatically altering their heating efficiency. By combining magneto-optical and fluorescence measurements, we demonstrate a form of biological microscopy that we used here to study the magnetization dynamics of nanoparticles in situ, in both histological samples and living cancer cells. Correlative magnetic and fluorescence imaging identified aggregated magnetic nanoparticles colocalized with cellular lysosomes. Nanoparticles aggregated within these lysosomes displayed reduced AC magnetic coercivity compared to the same particles measured in an aqueous suspension or aggregated in other areas of the cells. Such measurements reveal the power of this approach, enabling investigations of how cellular location, nanoparticle aggregation, and interparticle magnetic interactions affect the magnetization dynamics and consequently the heating response of nanoparticles in the biological milieu.

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Section: Discussionmentioning

confidence: 99%

Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples

Sharifabad,

Soucaille,

Wang

et al. 2024

ACS Nano

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“…It must also be noted that the spatial mapping of magnetic domains on a sample surface can be performed using Magnetic Force Microscopy (MFM) [89]. In its standard configuration, the MFM technique utilizes a probe coated with magnetic material to scan a sample using the non-contact or dynamic mode of atomic force microscopy (AFM) [89]. This approach entails monitoring the sample's surface features, where the probe comes into direct contact with the sample.…”

Section: Afm Imaging Artifactsmentioning

confidence: 99%

“…Previous studies on an MFM analysis of biological materials have revealed a significant challenge, namely, the potential contamination of the signal due to artifacts arising from topographical cross-talk. This has been demonstrated in samples comprising solid-state materials or nanoparticles [89]. To improve the efficiency of MFM, the effect of increasing scan rate has been previously explored [89].…”

Section: Afm Imaging Artifactsmentioning

confidence: 99%

“…This has been demonstrated in samples comprising solid-state materials or nanoparticles [89]. To improve the efficiency of MFM, the effect of increasing scan rate has been previously explored [89]. It was demonstrated that MFM images of tissue sections may exhibit contamination from artifacts caused by topographical cross-talk, particularly at elevated scan rates.…”

Section: Afm Imaging Artifactsmentioning

confidence: 99%

“…It was demonstrated that MFM images of tissue sections may exhibit contamination from artifacts caused by topographical cross-talk, particularly at elevated scan rates. These anomalies were noted both in rodent spleen samples and in sections of brain tissue extracted from Alzheimer's Disease patients [89]. It is also worth noting that traditional MFM necessitates multiple scans of the samples, is susceptible to various artifacts, and has constraints in its capacity for multimodal imaging or imaging in a fluid environment [98].…”

Section: Afm Imaging Artifactsmentioning

confidence: 99%

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Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers

Kontomaris,

Stylianou,

Chliveros

et al. 2023

Fibers

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Atomic force microscopy (AFM) is a powerful tool that enables imaging and nanomechanical properties characterization of biological materials. Nanofibers are the structural units of many biological systems and their role in the development of advanced biomaterials is crucial. AFM methods have proven to be effective towards the characterization of fibers with respect to biological and bioengineering applications at the nanoscale. However, both the topographical and mechanical properties’ nanocharacterizations of single fibers using AFM are challenging procedures. In particular, regarding imaging procedures, significant artifacts may arise from tip convolution effects. The geometrical characteristics of the AFM tip and the nanofibers, and the fact that they have similar magnitudes, may lead to significant errors regarding the topographical imaging. In addition, the determination of the mechanical properties of nanofibers is also challenging due to their small dimensions and heterogeneity (i.e., the elastic half-space assumption is not valid in most cases). This review elucidates the origins of errors in characterizing individual nanofibers, while also providing strategies to address limitations in experimental procedures and data processing.

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