Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells

Hwang, Jae Ho; Han, Uiyoung; Yang, Miso; Choi, Yonghyun; Choi, Jonghoon; Lee, Jong Min; Jung, Han‐Sung; Hong, Jinkee; Hong, Jeong Ho

doi:10.1016/j.actbio.2018.12.044

Cited by 28 publications

(17 citation statements)

References 36 publications

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“…Recent studies observed that the number of nuclear YAP1-expressing cells (52.43%) in OA cartilage was significantly higher than that detected in normal cartilage (8.39%), while the localization of cytoplasmic YAP1 was significantly lower than that observed in normal cartilage (20.02% in OA and 76.39% in normal cartilage) (Zhang et al, 2020). The level of YAP1 protein in the synovial fluid of patients with knee OA was observed to be significantly higher than that of a control group, suggesting that YAP1 protein may be an important factor in the pathogenesis of knee OA (Hwang et al, 2019). These results showed that YAP1 is significantly expressed at high levels in the OA model, suggesting that it may be involved in the development of OA and that inhibiting YAP1 expression may aid in inhibiting the development of OA.…”

Section: Yap1 Is An Important Promoter Of Oamentioning

confidence: 55%

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

Xie

Xiao

Tang

et al. 2020

Front. Cell Dev. Biol.

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The Hippo/yes-associated protein 1 signaling pathway is an evolutionarily conserved signaling pathway. This signaling pathway is primarily involved in the regulation of stem cell self-renewal, organ size and tissue regeneration by regulating cell proliferation, differentiation and apoptosis. It plays an important role in embryonic development and tissue organ formation. Yes-associated protein 1 (YAP1) is a key transcription factor in the Hippo signaling pathway and is negatively regulated by this pathway. Changes in YAP1 expression levels affect the occurrence and development of a variety of tumors, but the specific mechanism associated with this phenomenon has not been thoroughly studied. Recently, several studies have described the role of YAP1 in osteoarthritis (OA). Indeed, YAP1 is involved in orthopedic degenerative diseases such as osteoporosis (OP) in addition to OA. In this review, we will summarize the significance of YAP1 in orthopedic degenerative diseases and discuss the potential of the targeted modulation of YAP1 for the treatment of these diseases.

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Section: Yap1 Is An Important Promoter Of Oamentioning

confidence: 55%

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

Xie

Xiao

Tang

et al. 2020

Front. Cell Dev. Biol.

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“…Polyelectrolytes can be generally divided into two types according to their origins: synthetic and natural polyelectrolytes ( Table 2 ). [ 44–64 ] The most commonly used synthetic polyelectrolytes are poly‐ l ‐lysine (PLL), poly‐ l ‐arginine (PLA), polydiallyldimethyl ammonium chloride (PDADMAC), and polystyrene sulfonate (PSS). For example, Fournier and co‐workers tested several polyelectrolyte pairs for the construction of polymeric nanocoats on MELN cells through LbL technique.…”

Section: Materials and Strategies For Mammalian‐cell Nanocoatingmentioning

confidence: 99%

A Decade of Advances in Single‐Cell Nanocoating for Mammalian Cells

Lee

Kim

Rheem

et al. 2021

Adv Healthcare Materials

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Strategic advances in the single‐cell nanocoating of mammalian cells have noticeably been made during the last decade, and many potential applications have been demonstrated. Various cell‐coating strategies have been proposed via adaptation of reported methods in the surface sciences and/or materials identification that ensure the sustainability of labile mammalian cells during chemical manipulation. Here an overview of the methodological development and potential applications to the healthcare sector in the nanocoating of mammalian cells made during the last decade is provided. The materials used for the nanocoating are categorized into polymers, hydrogels, polyphenolic compounds, nanoparticles, and minerals, and the corresponding strategies are described under the given set of materials. It also suggests, as a future direction, the creation of the cytospace system that is hierarchically composed of the physically separated but mutually interacting cellular hybrids.

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“…An improved cell interaction between seeding cells and scaffold will greatly increase the efficiency of stem cell therapy, especially for the BTE field. Several recent studies have indicated that altered surface properties could stimulate hMSCs for osteogenic differentiation via nano-structure modification [10][11][12], surface coating [13], or topographical features [14]. The utilization of small amounts of surfactant on scaffold materials could achieve significant increases of hydrophilicity.…”

Section: Introductionmentioning

confidence: 99%

Altered Surface Hydrophilicity on Copolymer Scaffolds Stimulate the Osteogenic Differentiation of Human Mesenchymal Stem Cells

et al. 2020

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Background: Recent studies have suggested that both poly(l-lactide-co-1,5-dioxepan-2-one) (or poly(LLA-co-DXO)) and poly(l-lactide-co-ε-caprolactone) (or poly(LLA-co-CL)) porous scaffolds are good candidates for use as biodegradable scaffold materials in the field of tissue engineering; meanwhile, their surface properties, such as hydrophilicity, need to be further improved. Methods: We applied several different concentrations of the surfactant Tween 80 to tune the hydrophilicity of both materials. Moreover, the modification was applied not only in the form of solid scaffold as a film but also a porous scaffold. To investigate the potential application for tissue engineering, human bone marrow mesenchymal stem cells (hMSCs) were chosen to test the effect of hydrophilicity on cell attachment, proliferation, and differentiation. First, the cellular cytotoxicity of the extracted medium from modified scaffolds was investigated on HaCaT cells. Then, hMSCs were seeded on the scaffolds or films to evaluate cell attachment, proliferation, and osteogenic differentiation. The results indicated a significant increasing of wettability with the addition of Tween 80, and the hMSCs showed delayed attachment and spreading. PCR results indicated that the differentiation of hMSCs was stimulated, and several osteogenesis related genes were up-regulated in the 3% Tween 80 group. Poly(LLA-co-CL) with 3% Tween 80 showed an increased messenger Ribonucleic acid (mRNA) level of late-stage markers such as osteocalcin (OC) and key transcription factor as runt related gene 2 (Runx2). Conclusion: A high hydrophilic scaffold may speed up the osteogenic differentiation for bone tissue engineering.

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Artificial cellular nano-environment composed of collagen-based nanofilm promotes osteogenic differentiation of mesenchymal stem cells

Cited by 28 publications

References 36 publications

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

A Decade of Advances in Single‐Cell Nanocoating for Mammalian Cells

Altered Surface Hydrophilicity on Copolymer Scaffolds Stimulate the Osteogenic Differentiation of Human Mesenchymal Stem Cells

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