Artificial Gastrointestinal Models for Nutraceuticals Research—Achievements and Challenges: A Practical Review

Gościniak, Anna; Eder, Piotr; Walkowiak, Jarosław; Cielecka‐Piontek, Judyta

doi:10.3390/nu14132560

Cited by 18 publications

(6 citation statements)

References 111 publications

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“…The growing interest in these models arises from the need to circumvent the main limitations of working with animals and humans. In fact, working in vitro offers significant advantages, including high experimental reproducibility, continuous monitoring of culture conditions, convenient accessibility, cost-effectiveness, and avoidance of ethical concerns [ 20 ]. However, these advantages are attained by creating a working environment that is comparatively less intricate than the complex intestinal setting.…”

Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Calvigioni,

Mazzantini,

Celandroni

et al. 2023

Microorganisms

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Examining the interplay between intestinal pathogens and the gut microbiota is crucial to fully comprehend the pathogenic role of enteropathogens and their broader impact on human health. Valid alternatives to human studies have been introduced in laboratory practice to evaluate the effects of infectious agents on the gut microbiota, thereby exploring their translational implications in intestinal functionality and overall health. Different animal species are currently used as valuable models for intestinal infections. In addition, considering the recent advances in bioengineering, futuristic in vitro models resembling the intestinal environment are also available for this purpose. In this review, the impact of the main human enteropathogens (i.e., Clostridioides difficile, Campylobacter jejuni, diarrheagenic Escherichia coli, non-typhoidal Salmonella enterica, Shigella flexneri and Shigella sonnei, Vibrio cholerae, and Bacillus cereus) on intestinal microbial communities is summarized, with specific emphasis on results derived from investigations employing animal and in vitro models.

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Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Calvigioni,

Mazzantini,

Celandroni

et al. 2023

Microorganisms

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“…In this study, we assessed the effects of two phytogenic blends (PB1 and PB2), and their combination with casein on swine gut microbiota in an artificial, dynamic, computer-controlled in vitro large intestine system. Artificial GIT systems are intended to mimic the intestinal physiology of humans and animals, which makes them suitable models for pre-clinical and in vitro studies of the digestibility of foods, feed additives, and drugs and their effects on gut microbiota [ 25 , 26 , 27 ]. Validated artificial GIT models allow for conducting controlled, reproducible, and cost-effective studies, which sometimes are not achievable with in vivo models due to unpredictable factors, such as infections, uncontrolled animal welfare violations, and death of laboratory animals.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Swine Gut Microbiota by Phytogenic Blends and High Concentrations of Casein in a Validated Swine Large Intestinal In Vitro Model

Popov,

Einhardt Manzke,

Sost

et al. 2023

Veterinary Sciences

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Phytogenic feed additives are gaining popularity in livestock as a replacement for antibiotic growth promotors. Some phytogenic blends (PB) positively affect the production performance, inhibit pathogens within the gut microbiota, and improve the overall health of farm animals. In this study, a swine large intestine in vitro model was used to evaluate the effect of two PBs, alone or in combination with casein, on swine gut microbiota. As a result, the combination of casein with PB1 had the most beneficial effects on swine gut microbiota, as it increased the relative abundance of some commensal bacteria and two genera (Lactobacillus and Oscillospiraceae UCG-002), which are associated with greater production performance in pigs. At the same time, supplementation with PBs did not lead to an increase in opportunistic pathogens, indicating their safety for pigs. Both PBs showed fewer changes in swine gut microbiota compared to interventions with added casein. In contrast, casein supplementation significantly increased beta diversity and the relative abundance of commensal as well as potentially beneficial bacteria. In conclusion, the combination of casein with PBs, in particular PB1, had the most beneficial effects among the studied supplements in vitro, with respect to microbiota modulation and metabolite production, although this data should be proven in further in vivo studies.

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“…These mutants were adapted to the conditions of the adult colon in the PolyFermS platform [ 54 ]. Gosciniak, A. et al highlight their importance in contributing to disease understanding and present in vitro models enabling the study of microbiota under near-natural conditions [ 55 ]. In the study by Naimi, S. et al, the impact of Microcin J25 on the composition and metabolic activity of swine colonic microbiota is assessed.…”

Section: In Vitro Digestion Modelsmentioning

confidence: 99%

New Horizons in Probiotics: Unraveling the Potential of Edible Microbial Polysaccharides through In Vitro Digestion Models

Wang,

Zhu,

Zhang

et al. 2024

Foods

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In vitro digestion models, as innovative assessment tools, possess advantages such as speed, high throughput, low cost, and high repeatability. They have been widely applied to the investigation of food digestion behavior and its potential impact on health. In recent years, research on edible polysaccharides in the field of intestinal health has been increasing. However, there is still a lack of systematic reviews on the application of microbial-derived edible polysaccharides in in vitro intestinal models. This review thoroughly discusses the limitations and challenges of static and dynamic in vitro digestion experiments, while providing an in-depth introduction to several typical in vitro digestion models. In light of this, we focus on the degradability of microbial polysaccharides and oligosaccharides, with a particular emphasis on edible microbial polysaccharides typically utilized in the food industry, such as xanthan gum and gellan gum, and their potential impacts on intestinal health. Through this review, a more comprehensive understanding of the latest developments in microbial polysaccharides, regarding probiotic delivery, immobilization, and probiotic potential, is expected, thus providing an expanded and deepened perspective for their application in functional foods.

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Artificial Gastrointestinal Models for Nutraceuticals Research—Achievements and Challenges: A Practical Review

Cited by 18 publications

References 111 publications

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Modulation of Swine Gut Microbiota by Phytogenic Blends and High Concentrations of Casein in a Validated Swine Large Intestinal In Vitro Model

New Horizons in Probiotics: Unraveling the Potential of Edible Microbial Polysaccharides through In Vitro Digestion Models

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