Artificial hybridization of Japanese and European eel (Anguilla japonica×A. anguilla) by using cryopreserved sperm from freshwater reared males

Müller, Tamás; Horváth, Ákos; Takahashi, Eisuke; Kolics, Balázs; Bakos, Katalin; Decsi, K.; Kovács, Balázs; Taller, János; Urbányi, Béla; Bercsényi, Miklós; Horváth, László; Adachi, Souichi; Arai, Katsutoshi; Yamaha, Etsuro

doi:10.1016/j.aquaculture.2012.04.007

Cited by 14 publications

(23 citation statements)

References 21 publications

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“…(), who used “Tanaka solution” and DMSO as cryoprotectant: the hatchability of eggs fertilized with cryopreserved sperm was generally low (2.4%–22.5%, n = 3 females) compared to fresh sperm (11.6%–32.5%). However, this was better than the previous result (Müller et al., ), where only a few hybrid larvae hatched (hatching rate <1%). Although the effects of the rearing water temperature and salinity on the rate of larvae malformation in Japanese eel were also observed by Kurokawa et al.…”

Section: Discussioncontrasting

confidence: 78%

“…The induction of sexual maturation in captivity is a long process promoted by weekly hormone injections. Synchronization of ovulation and spermiation is not easy, thus the cryopreservation of sperm would render the synchronization as unnecessary (Müller et al., ). Tanaka et al.…”

Section: Introductionmentioning

confidence: 99%

“…Müller et al. () applied modified Tanaka's extender containing 350 mmol/L glucose, 30 mmol/L Tris (pH 8.0) and methanol cryoprotectant for cryopreservation of European eel, Anguilla anguilla, sperm samples that was used for hybridization between Japanese eel and European eel, resulting in hatched hybrid larvae. Asturiano, Sørensen, Pérez, Lauesen, and Tomkiewicz () managed to acquire European eel larvae using 50 mmol/L NaCl, 100 mmol/L NaHCO 3 , 2.5 mmol/L MgCl 2 , 1 mmol/L CaCl 2 , 30 mmol/L KCl, pH 6.5, mOsm/kg ~330 as media and DMSO 10% (v/v) and 25% foetal bovine serum (w/v).…”

Section: Introductionmentioning

confidence: 99%

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Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples

et al. 2017

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Summary The aim of this study was to test the artificial seminal plasma (ASP) as the extender as well as methanol as the cryoprotectant for cryopreservation, and to collect information on the fertilizing capacity of cryopreserved sperm samples. Eggs from Japanese eel, Anguilla japonica, females were control‐fertilized with native sperm diluted with ASP and cryopreserved sperm (ASP as extender [in 1:100 ratio] and 10% methanol in v/v final concentration). No statistical differences (p < .05) were among the measured parameters (hatching, survival after 10 days post‐hatch, malformation rates) between the two groups, thus this cryopreservation method can be used effectively for artificial propagation of the Japanese eel, Anguilla japonica.

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Section: Discussioncontrasting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples

et al. 2017

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“…In experiments which involved bringing European eel to maturity, its brooders were kept either in freshwater (Müller et al, 2005(Müller et al, , 2012Horvath et al, 2011;Prigge et al, 2012) or in saltwater (Epler and Bieniarz, 1978;Mordenti et al, 2013). Water salinity under controlled conditions can be changed very quickly -much more so than in the natural environment.…”

Section: Resultsmentioning

confidence: 99%

Changes in Body Weight and Eye Size in Female European Eel Kept in Fresh and Salt Water

Nowosad

Kucharczyk

Czarkowski

et al. 2014

Italian Journal of Animal Science

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The eel is a catadromous fish which spends most of its life in freshwater and adults swim to the Sargasso Sea region to spawn. While preparing for the reproductive process, eels undergo a metamorphosis to become what is called silver eel; a process involving changes in the colour and weight of the body and an increase in the eye size. These are indicators of fish maturity and they facilitate the selection of fish for reproduction under controlled conditions. During this study, changes in the body weight (BW) and eye size in female European eel were observed while being given weekly hormonal injections of 20 mg kg -1 carp pituitary homogenate and kept in 15°C freshwater and in 15°C saltwater with a salinity of 32-33‰. Fish kept in saltwater but not subjected to hormonal stimulation were used as a control group. Furthermore, after the experiment was finished, females in the control group were kept for next 5 months, with the same environmental parameters maintained. Differences between the treated groups were observed as early as week 4 of the experiment. An increase in fish BW was observed in fish kept both in salt and freshwater which were subjected to hormonal stimulation. On the other hand, changes in eye size were observed in the fish kept in saltwater, both in those hormonally stimulated and otherwise. The eye diameter in the fish kept in fresh after hormonal stimulation did not change significantly.

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“…For instance, cryopreservation of eel sperm has been reported for the first time in the Japanese eel Anguilla japonica (Tanaka et al, 2002). In the European eel ( Anguilla anguilla ), two teams started to work on the species independently of each other, a Spanish team building on the experiences of the previously mentioned experiments on the Japanese eel and developing their own media for cryopreservation (Peñaranda et al, 2009) and a Hungarian team building on their previous experience in common carp (Magyary et al, 1996) and later developing their methods based on those of Tanaka et al (2002), Müller et al (2012). This demonstrates the abundance of cryopreservation methods developed by several teams independently for a single species without intercalibration or standardization of their protocols.…”

Section: The Need For Standardizationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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Artificial hybridization of Japanese and European eel (Anguilla japonica×A. anguilla) by using cryopreserved sperm from freshwater reared males

Cited by 14 publications

References 21 publications

Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples

Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation using cryopreserved sperm samples

Changes in Body Weight and Eye Size in Female European Eel Kept in Fresh and Salt Water

Cryobanking of aquatic species

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