Artificial Insemination with Husband'S Sperm (Aih): Techniques for Sperm Selection

Pardo, M. C.; Bancells, N

doi:10.3109/01485018908986746

Cited by 13 publications

(5 citation statements)

References 44 publications

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“…A Percoll separation step was added to the sperm preparation protocol, and this treatment resulted in zona binding and acrosome reaction of a small number of control sperm. Percoll gradients have been utilized for human sperm preparation to enhance the percentage of motile sperm and to remove seminal plasma (Iizuka et al, 1988;Pardo and Bancells, 1989).…”

Section: Discussionmentioning

confidence: 99%

Separate effects of caffeine and dbcAMP on macaque sperm motility and interaction with the zona pellucida

VandeVoort

Tollner

Overstreet

1994

Molecular Reproduction Devel

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Capacitation of macaque sperm with caffeine and dbcAMP is required for fertilization in vitro. This study determined the separate effects of caffeine and dbcAMP on sperm-zona pellucida binding and the acrosome reaction of zona bound sperm. Semen from 6 cynomolgus macaques was washed through 60% Percoll, resuspended, and washed with BWW media and incubated for 2.5 hr. Caffeine, dbcAMP (2 mM each), or both (1 mM each) were added to aliquots of the sperm suspensions. Immature macaque oocytes were placed into drops of sperm suspensions, coincubated with sperm for 30 sec, and either fixed immediately or removed to sperm-free media and incubated 1 hr before fixation. There were no significant differences between groups in the percentage of live, acrosome-reacted sperm in suspension. Treatment with caffeine and dbcAMP or with caffeine alone, significantly increased the number of sperm bound to each zona pellucida (96 +/- 16 and 81 +/- 17, respectively) compared to control and dbcAMP treatment (15 +/- 4 and 28 +/- 13). However, treatment with dbcAMP, alone and with caffeine, resulted in a higher percentage of acrosome-reacted sperm on the zona (15.2 +/- 2.1 and 9.0 +/- 0.6) than control or caffeine treatment (3.0 +/- 1.4 and 2.4 +/- 0.5). Effects on sperm motility consistent with hyperactivation were detected only when both caffeine and dbcAMP were present. Although both caffeine and dbcAMP are presumed to increase or to produce the same effect as increased intracellular cAMP levels, these compounds have different effects on the ability of sperm to bind to the zona and to undergo the acrosome reaction.

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Section: Discussionmentioning

confidence: 99%

Separate effects of caffeine and dbcAMP on macaque sperm motility and interaction with the zona pellucida

VandeVoort

Tollner

Overstreet

1994

Molecular Reproduction Devel

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“…The ideal sperm preparation technique is the one which will achieve the largest number of morphologically normal motile spermatozoa in a small volume of physiological culture media, free from seminal plasma, leukocytes and bacteria [15]. There is more information than formerly about sperm quality and quantity necessary in an initial specimen for IUI to be successful, so that an informed decision can be made on whether to perform IUI or, instead, to recommend IVF with intracytoplasmic sperm injection (ICSI) or donor sperm [16].…”

Section: Sperm Preparationmentioning

confidence: 99%

“…Varicocele is the most common correctable cause of male infertility, and 90% occur on the left side owing to the anato my of entering the renal vein rather than the vena cava on the right side. The prevalence of varicocele in infertile men is 20-40% [15]. Authorities theorize that increased temperature, hypoxia and reflux of adrenal and renal metabolites may impair spermatogenesis in patients with varicocele.…”

Section: Varicocelementioning

confidence: 99%

“…The majority of men who have varicoceles are fertile, but any subfertile man with varicocele should be considered for repair, as 70% of men receiving surgical repair have significant improvement in semen parameters. Motility improvements are most common (70%), followed by improved sperm densities (51%) and improved morphology (44%), all of which increase the overall success rate of intrauterine insemination (IUI) [15]. Conception rates following varicocele repair average 40-50% when female factors are not present or have been appropriately treated .…”

Section: Chaptermentioning

confidence: 99%

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An overview of intrauterine insemination and ovulation induction

Brinsden¹,

Dickey²

2009

Manual of Intrauterine Insemination and Ovulation Induction

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(ICI) is to reduce the effect of factors such as vaginal acidity and cervical mucus hostility and to benefit from the deposition of a bolus of prepared motile, morphologically normal sperm as close as possible to the oocytes at the time of ovulation. There continues to be discussion in the literature about whether or not IUI should be complemented by OI-either with the oral medications clomiphene citrate (CC) or tamoxifen (TMX), or with the injectable gonadotropins. Most practitioners are of the opinion that IUI with OI does increase success rates, and many will initially try with CC or TMX, and move on to gonadotropins if there is no success within a few cycles of CC/IUI. The most appropriate time to move on from IUI to IVF is also a matter for debate, but most practitioners agree that the change should be made after no more than 4-6 cycles of IUI (see Chapters 7 and 8). Before proceeding to artificial insemination, couples should undergo a complete assessment, of which a full description is given in Chapters 2 and 3. This includes a thorough medical history, clinical examination and appropriate investigations for any possible causes of a couple's infertility, such as tubal damage, ovulatory disorder or a male factor. It is essential that couples should receive adequate counseling prior to starting treatment, especially when donor sperm is to be used. Couples should also be assured of complete confidentiality, and informed that all sperm donors are now comprehensively screened for genetic and infective conditions. Couples will wish to know how the donor is to be matched to their own characteristics, the cost of treatment, the probability of success, the potential for complications to occur and the likelihood of their occurrence. Medical professionals and couples can now make use of the internet to find a sperm donor that matches their desired physical, educational, religious and even national and ethnic

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“…Although the presence of such antibodies is especially prevalent in patients who have undergone vasectomy reversal, this condition may also be found to be increased in the general infertile male population. 9 A variety of techniques have been used in the treatment of antisperm antibodies, including high-dose oral corticosteroids of various concentrations and durations; sperm processing, 10 such as sperm washing [11][12][13] ; and modified methods of semen collection, such as ejaculation directly into buffered medium. Although the results of these techniques have been variable, no modality used to date has resulted in a consistently high degree of success.…”

Section: Evaluation Of the Male Before Ivf-etmentioning

confidence: 99%