This study assessed the physicochemical and microbiological quality of sachet drinking water samples in Kumasi, a major city in Ghana. Samples were collected from various sources within the city. Physicochemical properties, including pH, total dissolved solids, and concentrations of calcium, sodium, potassium, and magnesium ions, were analyzed following established protocols. Additionally, fluoride concentration was determined. The assessment criteria for water quality were based on the World Health Organization’s and the Ghana Standards Authority’s recommended standards for drinking water. The samples were also subjected to microbial analysis to detect the presence of E. coli and coliforms, and to evaluate microbial quality. The findings indicated that most physicochemical properties of the samples met the World Health Organization’s standards for safe drinking water, except for the slightly acidic pH. Total dissolved solids and the concentrations of calcium, sodium, potassium, fluoride and magnesium ions were within acceptable ranges. Strong positive correlations were observed among various physicochemical parameters of sachet water. However, microbial analysis revealed that 67% of the samples were contaminated with pathogenic microorganisms, including E. coli and coliforms, indicating poor microbiological quality. While sachet water samples generally meet physicochemical safety standards, addressing microbial quality is essential to ensure the safety of drinking water in Kumasi.