The multidisciplinary field of bioinformatics integrates information technology, biology, and computer science to analyze and comprehend biological data. It entails the creation and use of computational methods and tools to comprehend biological relationships and processes. The incorporation of bioinformatics into the field of food and nutritional science is examined in this chapter, with a focus on how it has revolutionized both research and practical applications. A thorough review of food science and nutritional research is given in the introduction, laying the groundwork for understanding how bioinformatics advances both disciplines. The foundations of bioinformatics are examined, together with significant databases and computing instruments required for data processing and interpretation. This chapter explores the various uses of bioinformatics in food and nutritional research, highlighting developments that have advanced these fields.