Background Artificial intelligence (AI) Healthcare applications are listed in the national visions of some Gulf Cooperation Council countries. A successful use of AI depends on the attitude and perception of medical experts of its applications. Objective To evaluate physicians and medical students’ attitude and perception on AI applications in healthcare. Method A web-based survey was disseminated by email to physicians and medical students. Results A total of 293 (82 physicians and 211 medical students) individuals have participated (response rate is 27%). Seven participants (9%) reported knowing nothing about AI, while 208 (69%) were aware that it is an emerging field and would like to learn about it. Concerns about AI impact on physicians’ employability were not prominent. Instead, the majority (n=159) agreed that new positions will be created and the job market for those who embrace AI will increase. They reported willingness to adapt AI in practice if it was incorporated in international guidelines (30.5%), published in respected scientific journals (17.1%), or included in formal training (12.2%). Almost two of the three participants agreed that dedicated courses will help them to implement AI. The most commonly reported problem of AI is its inability to provide opinions in unexpected scenarios. Half of the participants think that both the manufacturer and physicians should be legally liable for medical errors occur due to AI-based decision support tools while more than one-third (36.77%) think that physicians who make the final decision should be legally liable. Senior physicians were found to be less familiar with AI and more concerned about physicians’ legal liability in case of a medical error. Conclusion Physicians and medical students showed positive attitudes and willingness to learn about AI applications in healthcare. Introducing AI learning objectives or short courses in medical curriculum would help to equip physicians with the needed skills for AI-augmented healthcare system.