In view of the poor initialization performance of ecological environment monitoring network node location, a method of ecological environment monitoring network node location based on Zigbee is proposed. The node data collection model of ecological environment monitoring network is built based on Zigbee, and the performance is stable, which is more suitable for the node location of the ecological environment monitoring network; it is hoped that this study can provide reliable value reference and help for the future ecological studies. Through the installation of different types of sensors, the data of the ecological environment monitoring network nodes are automatically collected and sent to the server. The static weight coefficient of the collected data of the ecological environment monitoring network nodes is modified. According to the modified results, the ecological environment monitoring network is modified by DV-HOP positioning algorithm. The nodes of the ecological environment monitoring network are located by the three-way positioning method. The experimental results show that the initialization performance of this method is better, the accuracy is about 98%, and it is stable. It is more suitable for the node location of ecological environment monitoring network, which mainly includes ZigBee wireless sensor network module, embedded ARM, and Linux.