Artificial lipid–protein complexes accelerate cholesterol crystallisation in model bile

Hrbasová, Markéta; Vondrušková, Radka; Spundová, M; Marecek, Z; Entlicher, G.

doi:10.1016/s1357-2725(00)00009-1

Cited by 4 publications

(8 citation statements)

References 11 publications

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“…This site has been identified as a possible primary binding site of both bile salts [4,9,10] and Ca 2+ ion [8]. This site has been identified as a possible primary binding site of both bile salts [4,9,10] and Ca 2+ ion [8].…”

Section: Discussionmentioning

confidence: 94%

“…An artificial lipid-protein complex was prepared and this complex exerted a crystallization-promoting activity similar to that of the natural lipid-protein complex isolated from human bile [4]. HSA, TDC and Ca 2+ ions were used as the principal components of this complex.…”

Section: Discussionmentioning

confidence: 99%

“…The affinity of some drugs for binding Site-I on the albumin molecule was probably the cause of this effect. This site has been identified as a possible primary binding site of both bile salts [4,9,10] and Ca 2+ ion [8]. In addition, the TDC-HSA-Ca 2+ complex has been found to be a more potent accelerator of cholesterol crystallization than the TDC-HSA complex.…”

Section: Discussionmentioning

confidence: 99%

“…With the use of artificial lipid-protein complexes, which can be easily standardized, a test system was suggested which could serve for testing substances for their cholesterol crystallization-inhibiting activity [4].…”

Section: Introductionmentioning

confidence: 99%

“…Studies by Sudlow et al [6] led to the classification of drug binding sites on HSA molecule into two categories, which were denoted Site-I and Site-II. Among many other substances ethanol [7], Ca 2+ ions [8] and possibly bile salts [4,9,10] were found to bind to Site-I.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of drugs on cholesterol crystallization in an artificial bile model and relation of this effect to drug binding to albumin

Šmídková¹,

Spundová²,

Marecek

et al. 2003

Fundamemntal Clinical Pharma

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Substances that can affect the crystallization of cholesterol from human bile and consequently the gallstone formation have been given considerable attention. We improved the model system for testing cholesterol crystallization-affecting activity (promoting or inhibiting) of substances and used it for some drugs that are excreted into bile. Besides other factors natural lipid-protein complexes isolated from the native human bile have been shown to be responsible for nucleation and fast crystal growth in cholesterol supersaturated model bile. Artificial lipid-protein complex of taurolithocholate, human serum albumin and Ca2+ (TLTC-HSA-Ca2+) exhibited a lower crystallization activity than both the artificial lipid-protein complexes of taurodeoxycholate, human serum albumin and Ca2+ and the lipid-protein complex isolated from native human bile. The model bile supplemented with this artificial lipid-protein complex (TLTC-HSA-Ca2+) formed a convenient system for testing of various substances (drugs) for their crystallization-affecting activity. From the 20 tested drugs, which could occur at least in small amounts in human bile, the highest crystallization-promoting activity was found for complexes with ampicillin, butorphanol and colchicine. Complexes with tetracycline, thioridazine and doxycycline were the strongest inhibitors. The drugs, which had some effect on cholesterol crystallization, affected somehow the artificial lipid-albumin complex by displacing its components. Interactions of different drugs with HSA and its artificial complexes with the conjugated bile salt and Ca2+ ions were followed by absorption spectroscopy to observe displacement interactions. On the basis of these experiments we could classify drugs into four groups which differ by their effects on spectral characteristics of complexes.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of drugs on cholesterol crystallization in an artificial bile model and relation of this effect to drug binding to albumin

Šmídková¹,

Spundová²,

Marecek

et al. 2003

Fundamemntal Clinical Pharma

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Vibrational spectroscopic study of human pigment gallstones and their insoluble materials

Liu

Xing

Wang

et al. 2002

Journal of Molecular Structure

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Synthesis of Calcite with Novel Morphologies and Epitaxial Growth Calcium Bilirubinate (CaBR) on Calcite and Effects of Dipalmitoylposphatidylcholine (DPPC) Monolayer and Dextran

Huang

Shen

Xie

et al. 2008

Crystal Growth & Design

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Controlled growth of calcium carbonate crystals with complex forms was achieved using dextran as a crystal growth modifier beneath a dipalmitoylphosphatidylcholine (DPPC) monolayer, and calcite-core-calcium bilirubinate (CaBR)-side multiple crystals were also observed owing to the epitaxial growth of CaBR crystals on calcite. During the process, interactions between the DPPC monolayer and calcium ions were studied based on the analysis of the surface pressure−molecular area isotherms. Control over the crystallization of calcite with novel morphologies, such as octahedral crystals and round calcite disks, has been observed. Remarkable morphological changes and continuous structural evolution of calcite from rhombohedral crystal to octahedral crystal to uniform round disks and polyhedrons as concentrations of dextran increased have been observed. Moreover, semirigid multiplex templates consisting of calcium carbonate crystals and DPPC monolayers were established under physiological pH conditions, and epitaxial growth of sheet calcium bilirubinate crystals on calcite had been observed to form calcite-core-CaBR-side multiple crystals. The dynamic process of formation of multiple crystals was analyzed by monitoring the morphologies and components of crystals in different crystal stages. The possible formation mechanism of polymorphous calcite and calcite-core-CaBR-side crystal has been proposed, which provides an important insight into the epitaxial relationships between the surfaces of inorganic and bioorganic complex crystals and affords a detailed nanoscale understanding of how inorganic seeds may have a significant effect on crystal nucleation and growth processes.

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Artificial lipid–protein complexes accelerate cholesterol crystallisation in model bile

Cited by 4 publications

References 11 publications

Effect of drugs on cholesterol crystallization in an artificial bile model and relation of this effect to drug binding to albumin

Effect of drugs on cholesterol crystallization in an artificial bile model and relation of this effect to drug binding to albumin

Vibrational spectroscopic study of human pigment gallstones and their insoluble materials

Synthesis of Calcite with Novel Morphologies and Epitaxial Growth Calcium Bilirubinate (CaBR) on Calcite and Effects of Dipalmitoylposphatidylcholine (DPPC) Monolayer and Dextran

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