Artificial Liver Support Systems: Design of Preclinical Evaluation Programmes

Ryan, C. J.; Aslam, Mohammed; Cohen, Jon; Courtney, J. M.

doi:10.1007/978-1-349-09609-1_11

Cited by 1 publication

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“…The basis of our approach to artificial liver support has been the utilization of extracorporeal circuits in the rat in order to study the relationship between liver support systems, hepatic coma, detoxification, and liver regeneration (8,9,(11)(12)(13)(18)(19)(20)(21)(22). The suitability of the membrane plasma separation stage for the evaluation of single and/or multiple sorbents (multisorbent regimen) in normal animals and those with experimentally induced acute FHF was reported in detail elsewhere (8,9,12,22).…”

Section: Discussionmentioning

confidence: 99%

Multisorbent Plasma Perfusion in Fulminant Hepatic Failure: Effects of Duration and Frequency of Treatment in Rats with Grade III Hepatic Coma

Ryan¹,

Anilkumar²,

Ben-Hamida³

et al. 2001

Artificial Organs

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Using the model of galactosamine-induced fulminant hepatic failure in the rat, the effects of multisorbent plasma perfusion over Asahi uncoated spherical charcoal, Plasorba (BR-350) resin, and an endotoxin removing adsorbent (polymyxin B-sepharose) were determined in Grade III hepatic coma animals by studying survival as influenced by timing, duration, and frequency of treatment. The effects of treatment on liver cell proliferation and endotoxin removal also were examined. The results demonstrate that duration and frequency of treatment are major contributing factors in the successful application of nonbiological membrane-based multisorbent liver support systems. Examination of the regenerative activity in the liver indicates an enhanced proliferative response following multisorbent plasma perfusion compared with untreated fulminant hepatic failure (FHF) paired controls. Utilizing an endotoxin removal adsorbent alone, a marked reduction in systemic levels of endotoxin in FHF was demonstrated compared with nonperfused FHF paired controls. Despite current emphasis on bioartificial liver support systems, plasma purification by multisorbent systems offers a simple method for the removal of circulating toxic metabolites in general together with specific toxin removal.

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