Artificial naringinase system for cooperative enzymatic synthesis of naringenin

Co-Immobilizing Two Glycosidases Based on Cross-Linked Enzyme Aggregates to Enhance Enzymatic Properties for Achieving High Titer Icaritin Biosynthesis

“…As shown in Figure C, the greatly enhanced activity recovery of immobilized Rha1 (1.17-fold of free Rha1) or Glu4 (1.6-fold of free Glu4) could be achieved by increasing the glutaraldehyde concentration up to 0.2 and 0.5% (v/v), respectively. According to Wang et al, only 37.3% of rhamnosidase activity and 56.3% of glycosidase activity were recovered by immobilizing on magnetic silica-based chitosan microspheres . The increase of enzyme activities might be due to diverse reasons.…”

“…According to Wang et al, only 37.3% of rhamnosidase activity and 56.3% of glycosidase activity were recovered by immobilizing on magnetic silica-based chitosan microspheres. 53 The increase of enzyme activities might be due to diverse reasons. One was the changes of enzyme conformations caused by cross-linking, exposing more catalytic sites.…”

Flavonoids as Aglycones in Retaining Glycosidase-Catalyzed Reactions: Prospects for Green Chemistry

“…In food technology, the removal or deglycosylation of naringin, one of the main contributors to bitterness in orange and grapefruit juices, has been described as a treatment that improves juice quality because the released aglycone naringenin is perceived as less bitter . As an example, the deglycosylation of naringin, leading to the release of β- d -glucose, α- l -rhamnose and naringenin, was performed with an α- l -rhamnosidase and a β- d -glucosidase, which were coimmobilized onto magnetic silica-based chitosan microspheres . Similarly, so-called naringinase, i.e., an enzyme preparation that contains α- l -rhamnosidase and β- d -glucosidase activities, was immobilized onto graphene oxide for the production of prunin and naringenin from naringin with excellent process stability .…”

“… 40 As an example, the deglycosylation of naringin, leading to the release of β- d -glucose, α- l -rhamnose and naringenin, was performed with an α- l -rhamnosidase and a β- d -glucosidase, which were coimmobilized onto magnetic silica-based chitosan microspheres. 41 Similarly, so-called naringinase, i.e., an enzyme preparation that contains α- l -rhamnosidase and β- d -glucosidase activities, was immobilized onto graphene oxide for the production of prunin and naringenin from naringin with excellent process stability. 42 Grapefruit pith is a rich source of naringin and narirutin and is a valuable byproduct of the fruit juice-producing industry.…”