Biodiesel is an alternative fuel for diesel engine. Considering the differences between diesel and biodiesel fuels, the engine condition should be modified based on the fuel or fuel blends to achieve optimum performance. This study presented a performance analysis of a direct-injected (DI) diesel engine with a dynamometer fueled with diesel-tomato seed biodiesel (TSOB) blends employing ANOVA and universal nonlinear model based on ANN. The experiments were carried out under conditions of some independent variables including different engine loads (0, 50, 100%) and speed (1800, 2150, and 2500 rpm) for four diesel-biodiesel combinations (B0, B5, B10, and B20). In this research, the effect of these factors on dependent variables including power, torque, SFC, FC, and Exhaust Gas Temperature (EGT) are investigated. Duncan s multi-domain test at a significance level of R < 0.01 shows that the highest and lowest of the torque and power are produced from B5 and B20, respectively. These results show that the lowest EGT of 613 K is related to B20 and the highest EGT is related to B5 and B10. The regression models showed that the torque decreases with increasing the engine speed and biodiesel percentage. These results also show that the highest and the lowest SFC is related to B0 and B20, respectively. The ANN model shows high capability of predicting the engine performance parameters and emissions, without running costly and time-consuming experiments with the histogram error of 0.004 and R = 0.96. It also proved that ANN is a non-linear model of choice to deal with these data, instead of multivariate linear regression employed for preliminary analysis.Energies 2019, 12, 4421 2 of 18 tomato seed was found to contain 18-23% oil, which can be used to make biodiesel [6,7]. Biodiesel is compatible with conventional diesel and could be mixed with any ratio of diesel for running an engine [8,9]. The main differences between biodiesel and diesel are lower calorific value and the source from which these products are derived, and the manufacturing process behind their production, which limit the oxygen content. The amount of oxygen in diesel is zero, while it is 10 to 12% in biodiesel, and this reduces the energy density as well as reduces the emissions of suspended particles. Biodiesel is considered a clean fuel; however, research shows that biodiesel has a large Cetane Index which reduces the ignition time, but, on the other hand, its Lower Heating Value (LHV) is lower than that of diesel and thus produces lower power in the engine [10][11][12]. Renewability, lower amounts of combustion pollutants are one of the most important reasons for choosing the biodiesels as a substitute for diesel [13]. Approximately, one-third of the world s fossil fuels are consumed in internal combustion engines (ICEs), which emit harmful exhaust gases and are the main reasons for environmental pollution. Diesel engines operate relatively more efficiently than petrol engines. The current and future regulation of pollution requires exploration of fuels t...