Artificial selection of microbial communities: what have we learnt and how can we improve?

Thomas, Joshua L; Rowland-Chandler, Jamila; Shou, Wenying

doi:10.1016/j.mib.2023.102400

Cited by 8 publications

(1 citation statement)

References 43 publications

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“…The interest in microbial cocultures has been growing continuously in recent years, in concert with the achievements made with regard to the characterization of the intestinal microbiome, the construction of artificial microbial communities and the understanding of ecological interactions between microorganisms in the natural environment (Burz et al 2023 ; Fan and Pedersen 2021 ; Thomas et al 2024 ). The currently observed “paradigm shift” in microbiological sciences involves, among other factors, progressing from the conventional approach of investigating microbial monocultures (axenic cultures) toward employing cocultures of two or more species (or strains) that are free to interact with each other (Nai and Meyer 2018 ).…”

Section: Introductionmentioning

confidence: 99%

The repertoire and levels of secondary metabolites in microbial cocultures depend on the inoculation ratio: a case study involving Aspergillus terreus and Streptomyces rimosus

Boruta,

Englart,

Foryś

et al. 2024

Biotechnol Lett

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Objective The aim of this study was to determine the influence of the inoculation volume ratio on the production of secondary metabolites in submerged cocultures of Aspergillus terreus and Streptomyces rimosus. Results The shake flask cocultures were initiated by using 23 inoculum variants that included different volumes of A. terreus and S. rimosus precultures. In addition, the axenic controls were propagated in parallel with the cocultures. UPLC‒MS analysis revealed the presence of 15 secondary metabolites, 12 of which were found both in the “A. terreus vs. S. rimosus” cocultures and axenic cultures of either A. terreus or S. rimosus. The production of the remaining 3 molecules was recorded solely in the cocultures. The repertoire and quantity of secondary metabolites were evidently dependent on the inoculation ratio. It was also noted that detecting filamentous structures resembling typical morphological forms of a given species was insufficient to predict the presence of a given metabolite. Conclusions The modification of the inoculation ratio is an effective strategy for awakening and enhancing the production of secondary metabolites that are not biosynthesized under axenic conditions.

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