Artificial Smooth Muscle Model Composed of Hierarchically Ordered Microtubule Asters Mediated by DNA Origami Nanostructures

Matsuda, Kento; Kabir, Arif Md. Rashedul; Akamatsu, Naohide; Ai, Saito; Ishikawa, Shumpei; Matsuyama, Tsuyoshi; Ditzer, Oliver; Islam, Md. Sirajul; Ohya, Yusuke; Sada, Kazuki; Konagaya, Akihiko; Kuzuya, Akinori; Kakugo, Akira

doi:10.1021/acs.nanolett.9b01201

Cited by 56 publications

(52 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We then begin the modeling study of the microtubule mesh structure using Blender to evaluate the characteristics of the dynamics and we explore possible ways to improve the target molecular system. Currently, we well reproduced contraction of this artificial muscle that is initiated upon ATP addition, as described in the previous report [ 4 ]. We will also discuss a potential strategy to accomplish multiple time contractions by a quick recovery method based on the obtained dynamic property of this contractile material.…”

Section: Introductionmentioning

confidence: 65%

“…In nanotechnology, the mobile micromachine is gaining attention for potential application to the development of artificial muscle [ 1 , 2 , 3 ]. In particular, materials with microtubules and motor proteins have been demonstrated to provide contraction [ 4 ]. Motor proteins were designed to produce the pulling force between microtubule filaments that is known to happen in cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Modeling a Microtubule Filaments Mesh Structure from Confocal Microscopy Imaging

et al. 2020

Self Cite

View full text Add to dashboard Cite

This study introduces a modeling method for a supermolecular structure of microtubules for the development of a force generation material using motor proteins. 3D imaging by confocal laser scanning microscopy (CLSM) was used to obtain 3D volume density data. The density data were then interpreted by a set of cylinders with the general-purpose 3D modeling software Blender, and a 3D network structure of microtubules was constructed. Although motor proteins were not visualized experimentally, they were introduced into the model to simulate pulling of the microtubules toward each other to yield shrinking of the network, resulting in contraction of the artificial muscle. From the successful force generation simulation of the obtained model structure of artificial muscle, the modeling method introduced here could be useful in various studies for potential improvements of this contractile molecular system.

show abstract

Section: Introductionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Modeling a Microtubule Filaments Mesh Structure from Confocal Microscopy Imaging

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adapted with permission from ref. [48].C opyright:2 019,American Chemical Society. case, GOx and HRP,w ith d-glucose as ar eactanta nd 3,3',5,5'tetramethylbenzidine as ar eporter) and then madet hese two nanotubes dimerise on their ends ( Figure 6B).…”

Section: Biomimetic Dna Nanotechnology For Potential In Vivo Applicatmentioning

confidence: 99%

Advances in DNA Origami–Cell Interfaces

et al. 2019

View full text Add to dashboard Cite

The nascent field of DNA nanotechnology has undergone rapid growth since its inception. By using DNA as a biologically “safe” material, DNA nanotechnology shows great promise in applications such as drug‐delivery systems. Further progress, however, relies on understanding the different ways in which DNA nanostructures behave in and interact with cells, tissues and even whole organisms. Moreover, this knowledge must then be harnessed in innovative ways to improve existing DNA nanostructures and design new ones, so that they can perform more diverse functions more effectively. There have been many developments in this regard in the past few years, and herein some of these are highlighted, with respect to both works that improve our understanding of what happens to DNA nanostructures once they are at their target site, and those that utilise clever design to accomplish desired functions.

show abstract

“…Self-assembled MTs are attractive from a materials synthesis perspective, as MTs themselves are incredibly rigid, exceeding even single-walled carbon nanotubes in terms of their bending rigidity [14]. MTs have been considered for a wide range of applications, including tension sensors [15], lab-on-a-chip components [16], molecular robots, artificial muscles and others [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…The ability to control the self-assembly and orientation of MTs is of paramount importance for future envisioned technologies including sensors, molecular motors and actuators, protein-based engineered nanostructures, etc. [14][15][16][17][18][19][20] Previous studies on MT polymerization, with or without PTX, mainly reported turbidity or absorbance as the primary means of evaluating their time-dependent bvpolymerization [42]. These techniques are attractive because they are simple and applicable to cell biology and cancer research, but they do not provide information regarding the micro-or macroscopic organization of MTs.…”

Section: Introductionmentioning

confidence: 99%

Interplay between Convective and Viscoelastic Forces Controls the Morphology of In Vitro Paclitaxel-Stabilized Microtubules

Barnes

Guan

Alberts³

et al. 2020

Crystals

View full text Add to dashboard Cite

Microtubules (MTs) are self-assembling, high-aspect-ratio tubular nanostructures formed from the polymerization of tubulin protein. MTs are capable of globally assembling into optically birefringent morphologies, but there is disagreement on the mechanisms driving this behavior. We investigated the temporal evolution of paclitaxel (PTX)-stabilized MT solutions under a range of in vitro conditions. Significant morphological differences were observed in the polymerized PTX-MT solutions as a consequence of varying the orientation of the reaction vessel (vertical vs. horizontal), the type of heating source (hot plate vs. incubator), the incubation time, and the concentration of PTX (high vs. low). The most robust birefringent patterns were found only in vertically oriented cuvettes that were heated asymmetrically on a hot plate, suggesting dependence upon a convective flow, which we confirmed with a combination of optical and thermal imaging. Higher concentrations of PTX led to denser PTX-MT domain formation and brighter birefringence, due to more complete polymerization. Combining our experimental observations, we conclude that birefringent patterns arise principally through a combination of convective and viscoelastic forces, and we identify the sequence of dynamical stages through which they evolve.

show abstract

Artificial Smooth Muscle Model Composed of Hierarchically Ordered Microtubule Asters Mediated by DNA Origami Nanostructures

Cited by 56 publications

References 28 publications

Modeling a Microtubule Filaments Mesh Structure from Confocal Microscopy Imaging

Modeling a Microtubule Filaments Mesh Structure from Confocal Microscopy Imaging

Advances in DNA Origami–Cell Interfaces

Interplay between Convective and Viscoelastic Forces Controls the Morphology of In Vitro Paclitaxel-Stabilized Microtubules

Contact Info

Product

Resources

About