Artificially designed promoters

Baumann, Martina; Höppner, Marc P.; Meier, Michael; Pontiller, Jens; Ernst, Wolfgang; Grabherr, Reingard; Mauceli, Evan; Grabherr, Manfred

doi:10.4161/bbug.18530

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…The principles of synthetic biology have led to the creation of synthetic core promoters, synthetic promoters, employing the natural viral core, or those completely lacking homology with existing promoters …”

Section: Synthetic Promotersmentioning

confidence: 99%

“…The transcription levels of synthetic promoters Ar110S, Ar200S, Ar232S, and Ar300S were comparable to or have exceeded that of the SV40 core promoter used as a control. Introduction of regions rich in A and T alternating with CpG motifs can further increase the strength of synthetic promoters . Interestingly, the spatial arrangement of the AT islands plays a crucial role and can increase or decrease the strength of the synthetic promoter.…”

Section: Synthetic Promotersmentioning

confidence: 99%

See 1 more Smart Citation

Engineered and Natural Promoters and Chromatin‐Modifying Elements for Recombinant Protein Expression in CHO Cells

Romanova

Noll

2017

Biotechnology Journal

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Promoters play a pivotal role in integrating and processing the signals related to transcription initiation. Strong natural viral promoters, such as hCMV or SV40E, have been routinely employed to achieve a high rate of gene expression in ubiquitously used Chinese hamster ovary (CHO) cells. However, viral promoters are susceptible to epigenetic silencing and lack precise regulation levers. This has paved the way to more sensible control elements: endogenous, inducible, and synthetic promoters. In this review we summarize and discuss the use of natural viral, mammalian, and endogenous promoters, as well as recent advances in synthetic promoters and inducible systems for protein expression in CHO cells. Not only the level of transcription, but its long-term stability is crucial for recombinant protein production. Epigenetic chromatin-modifying elements, such as ubiquitously acting chromatin opening elements (UCOEs), matrix attachment regions (MARs), insulators and stabilizing anti-repressors (STARs) significantly improve transcription levels over extended cultivation time and are also discussed here. This review provides up-to date information to facilitate the choice of a suitable promoter and adjacent chromatin-modifying elements to maximize transgene expression as well as ensure long-term expression stability in CHO cell culture.

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Section: Synthetic Promotersmentioning

confidence: 99%

Section: Synthetic Promotersmentioning

confidence: 99%