Artificially-Synthesized Intergeneric Chimera between Brassica oleracea and Raphanus sativus by In Vitro Grafting.

Abstract

The first Intergeneric chimeras between radish and cabbage were successfully synthesized by in vitro grafting method. Regenerated seventy seven chimeras from the original sectorial chimera were investigated on morphological, physiological natures comparing with other interspecific chimeras of Brassica. Chimera! structures were justified and totally classified based on the morphological characteristics, isozymatic band patterns and PCR analysis. In those chimeras, putative four-layered chimeral plants were also found for two vegetatively-propagated generations with general threelayered chimeras. Physiological interactions were typically recognized such as in flowering date, pollen fertility, and pod and seed set. However, genetic changes were not found in the progeny derived from the crosses with both parents.

Production and characterization of intergeneric somatic hybrids between Moricandia arvensis and Brassica oleracea

“…Mitochondorial DNA was analysed by the PCR method according to Hirata et al (2000), using the three primers atp6, atp9 and Ogu atp6. In the PCR analysis, the amplification condition was same as that of Hirata et al (2001): 3 min of predenaturation at 94°C were followed by 30 cycles of denaturation at 94°C for 1 min, annealing at 60°C for 2 min, extension at 72°C for 3 min and prolongation at 72°C for 7 min PCR products were electrophoresed on 1.0% agarose gel and then stained by ethidium bromide.…”

Research Article Periclinal chimera technique: new plant breeding approach

“…Epigenetic interactions that caused complex heritable traits such as plant height have also been reported by Bomfim and Nassar (2014). It was found out that exchange of RNA and DNA to adjacent cells in grafts occurs (Hirata et al, 1999(Hirata et al, , 2000Stegemann and Bock, 2009;Nassar and Bomfim, 2013). In plant chimeras obtained by grafting, L1, L2, and L3 origins have also been identified by differential gene expression as well as the presence of different proteins in various histogenic-derived tissues (Filippis et al, 2013).…”

“…Plant chimeras were identified based on alterations in stems and leaf morphology. Stem and leaf size and shape were used to differentiate between chimeras and their donor parents using previously established methods (Carlson and Chaleff, 1974;Hirata et al, 2000;Nielsen et al, 2003;Hashimoto-Freitas and Nassar, 2013).…”

“…A chimera is a meristem with different genetic tissues in one or more of its layers. The components of plant grafts in a chimera do not lose their integrity but coexist harmoniously (Buder, 1911;Goffreda et al, 1990;Hirata et al, 2000).…”