Aryl Hydrocarbon Receptor Signaling Is Functional in Immune Cells of Rainbow Trout (Oncorhynchus mykiss)

Song, Jung‐Min; Casanova-Nakayama, Ayako; Möller, Anja-Maria; Kitamura, Shin-Ichi; Nakayama, Kei; Segner, Helmut

doi:10.3390/ijms21176323

Cited by 14 publications

(13 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With this approach, the authors identified B cells and granulocytes to be CYP1A-immunoreactive. This finding would agree with the FISH data of Song et al [146] on AhR cellular distribution. Phalen et al [152] applied FACS sorting of rainbow trout immune cell populations, and found that AhR and CYP1A mRNA were expressed in B cells of blood, spleen, and head kidney as well as in thrombocytes of blood and spleen.…”

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishsupporting

confidence: 93%

“…This has been shown in a number of in vivo stud-ies when fishes exposed to compounds, such as TCDD or BaP, showed increased CYP1A immunoreactivity and/or CYP1A mRNA in immune cells [151,152,[157][158][159][160]. Additionally, in vitro studies with isolated fish leukocytes show that exposure to AhR ligands results in CYP1A induction in these cells [146,150]. Importantly, CYP1A induction can be inhibited by antagonistic AhR ligands, such as α-napthoflavone [146], a finding that argues for functional involvement of the AhR signaling pathway.…”

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 73%

“…For the latter species, AhR1 and AhR2 protein were also present in the spleen [143]. In rainbow trout (Oncorhynchus mykiss), AhR mRNA was found in the head kidney and in the spleen [144][145][146]. The ratio of the expression levels of the AhR isoforms varies between tissues, for instance, in the rainbow trout spleen, the expression ratio between AhR2α and AhR2β was balanced, whereas in the heart, AhR2β transcripts showed a much higher abundance compared to AhR2α transcripts [144].…”

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 99%

“…Thus, studies on AhR expression at the organ level need to be broken down to studies on the expression and localization of the AhR isoforms at the level of the immune cells. Song et al [146] compared the transcript levels of AhR2α and AhR2β in the whole head kidney organ of rainbow trout and in leukocytes isolated from the head kidney, and found similar expression levels in both systems. Additionally, the AhR induction response to BaP exposure was comparable in the whole organ and the isolated immune cells.…”

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 99%

“…"Leukocytes" is a rather generic term; the question is which leukocyte subpopulations express the AhR. Song et al [146] observed by means of fluorescent in situ hybridization (FISH) that AhR mRNA was expressed not in all but only in specific immune cell populations of rainbow trout. Although identifying immune cell types without specific cell markers is difficult, the morphological appearance of the FISH-positive cells resembled lymphocytes and neutrophils.…”

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 99%

See 4 more Smart Citations

Immunotoxicity of Xenobiotics in Fish: A Role for the Aryl Hydrocarbon Receptor (AhR)?

Segner

Bailey²,

Tafalla³

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

The impact of anthropogenic contaminants on the immune system of fishes is an issue of growing concern. An important xenobiotic receptor that mediates effects of chemicals, such as halogenated aromatic hydrocarbons (HAHs) and polyaromatic hydrocarbons (PAHs), is the aryl hydrocarbon receptor (AhR). Fish toxicological research has focused on the role of this receptor in xenobiotic biotransformation as well as in causing developmental, cardiac, and reproductive toxicity. However, biomedical research has unraveled an important physiological role of the AhR in the immune system, what suggests that this receptor could be involved in immunotoxic effects of environmental contaminants. The aims of the present review are to critically discuss the available knowledge on (i) the expression and possible function of the AhR in the immune systems of teleost fishes; and (ii) the impact of AhR-activating xenobiotics on the immune systems of fish at the levels of immune gene expression, immune cell proliferation and immune cell function, immune pathology, and resistance to infectious disease. The existing information indicates that the AhR is expressed in the fish immune system, but currently, we have little understanding of its physiological role. Exposure to AhR-activating contaminants results in the modulation of numerous immune structural and functional parameters of fish. Despite the diversity of fish species studied and the experimental conditions investigated, the published findings rather uniformly point to immunosuppressive actions of xenobiotic AhR ligands in fish. These effects are often associated with increased disease susceptibility. The fact that fish populations from HAH- and PAH-contaminated environments suffer immune disturbances and elevated disease susceptibility highlights that the immunotoxic effects of AhR-activating xenobiotics bear environmental relevance.

show abstract

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishsupporting

confidence: 93%

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 73%

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 99%

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 99%

Section: The Presence and Xenobiotic Inducibility Of The Ahr In Immune Organs And Cells Of Teleost Fishmentioning

confidence: 99%

See 3 more Smart Citations

Immunotoxicity of Xenobiotics in Fish: A Role for the Aryl Hydrocarbon Receptor (AhR)?

Segner

Bailey²,

Tafalla³

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

Salinity‐induced transcriptome profiles in marine and freshwater threespine stickleback after an abrupt 6‐hour exposure

Taugbøl

Solbakken

Jakobsen

et al. 2022

Ecology and Evolution

View full text Add to dashboard Cite

Saltwater and freshwater environments have opposing physiological challenges, yet, there are fish species that are able to enter both habitats during short time spans, and as individuals they must therefore adjust quickly to osmoregulatory contrasts. In this study, we conducted an experiment to test for plastic responses to abrupt salinity changes in two populations of threespine stickleback, Gasterosteus aculeatus, representing two ecotypes (freshwater and ancestral saltwater). We exposed both ecotypes to abrupt native (control treatment) and non‐native salinities (0‰ and 30‰) and sampled gill tissue for transcriptomic analyses after 6 h of exposure. To investigate genomic responses to salinity, we analyzed four different comparisons; one for each ecotype (in their control and exposure salinity; (1) and (2), one between ecotypes in their control salinity (3), and the fourth comparison included all transcripts identified in (3) that did not show any expressional changes within ecotype in either the control or the exposed salinity (4)). Abrupt salinity transfer affected the expression of 10 and 1530 transcripts for the saltwater and freshwater ecotype, respectively, and 1314 were differentially expressed between the controls, including 502 that were not affected by salinity within ecotype (fixed expression). In total, these results indicate that factors other than genomic expressional plasticity are important for osmoregulation in stickleback, due to the need for opposite physiological pathways to survive the abrupt change in salinity.

show abstract

Molecular characterization of the aryl hydrocarbon receptor 2 gene in black rockfish, Sebastes schlegelii, and its expression patterns upon exposure to benzo[a]pyrene, 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin, and β‐naphthoflavone

Woo

2021

J of Applied Toxicology

View full text Add to dashboard Cite

The aryl hydrocarbon receptor (AhR) is a ligand‐activated transcription factor that mediates the toxicity of halogenated and polycyclic aromatic hydrocarbons in vertebrates. Thus, increased knowledge of AhR‐mediated responses to xenobiotics is imperative. Sebastes schlegelii is increasingly being used as a model for studying environmental toxicology; hence, in this study, the presence of AhR2 was evaluated in S. schlegelii. The results showed that the predicted AhR2 amino acid sequence contained regions characteristic of other vertebrate AhRs, including the basic helix–loop–helix and PER–ARNT–SIM domains in the N‐terminal half, but it had minor similarity with other vertebrate AhRs across the C‐terminal half; it did not contain the distinct glutamine‐rich domains found in mammalian AhR2. Phylogenetic analysis demonstrated that S. schlegelii AhR2 was clustered within the teleost AhR2 branch. Additionally, AhR2 mRNA was detectable in all 11 tissues tested, with the highest mRNA levels in the heart, pyloric ceca, and liver. Furthermore, exposure to the AhR agonists showed that 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD, 1 μg/g body weight) induced a significantly higher increases in AhR2 expression in the gills, liver, kidneys, and spleen in 48 h than benzo[a]pyrene (2 μg/g body weight), and β‐naphthoflavone (50‐μg/g body weight); AhR2 mRNA levels upon TCDD exposure were up‐regulated by 16‐ and 10‐fold in the gills and liver, respectively. These findings indicated that AhR was a highly sensitive receptor against TCDD. Thus, investigating AhR2 expression in the presence of other xenobiotics might offer further information for the elucidation of its crucial role in mediating toxicant metabolism in S. schlegelii.

show abstract

Aryl Hydrocarbon Receptor Signaling Is Functional in Immune Cells of Rainbow Trout (Oncorhynchus mykiss)

Cited by 14 publications

References 73 publications

Immunotoxicity of Xenobiotics in Fish: A Role for the Aryl Hydrocarbon Receptor (AhR)?

Immunotoxicity of Xenobiotics in Fish: A Role for the Aryl Hydrocarbon Receptor (AhR)?

Salinity‐induced transcriptome profiles in marine and freshwater threespine stickleback after an abrupt 6‐hour exposure

Molecular characterization of the aryl hydrocarbon receptor 2 gene in black rockfish, Sebastes schlegelii, and its expression patterns upon exposure to benzo[a]pyrene, 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin, and β‐naphthoflavone

Contact Info

Product

Resources

About