ARYLAZIDO AMINOPROPIONYL ATP (ANAPP<sub>3</sub>) ANTAGONISM OF CAT URINARY BLADDER CONTRACTIONS

Theobald, Robert J.

doi:10.1111/j.1474-8673.1982.tb00487.x

Cited by 36 publications

(14 citation statements)

References 6 publications

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“…Quinidine (a P2-receptor antagonist; Burnstock, 1980) also antagonized the nicotine response, an effect which could be due to the block of entry of nicotine into the noradrenergic or purinergic nerve terminals (see Theobald, 1983b). The principal active substance released by nonadrenergic non-cholinergic nerves is a purine nucleotide, probably ATP and the nerves have therefore been termed purinergic (Burnstock, 1972;1980;Theobald, 1982;1983a). The predominant response to ATP in smooth muscle is a relaxation; excitatory responses occur in the urinary bladder (Ambache & Zar, 1970;Burnstock et al, 1972;Creed & Tulloch, 1982).…”

Section: Discussionmentioning

confidence: 99%

The effects of nicotine on spontaneous contractions of cat urinary bladder in situ

Koley

Saha

1984

British J Pharmacology

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1 Nicotine and dimethyl-phenylpiperazinium (DMPP) increased intravesicular pressure and then transiently depressed the spontaneous activity of the urinary bladder in chloralose anaesthetized cats.2 Adrenaline (5-10tgkg-1), noradrenaline (5-20pgkg-1) and isoprenaline (40-50 gkg-') which depressed spontaneous urinary bladder activity, were antagonized by the ,3-receptor blocking agent propranolol (1 mg kg-). Phenylephrine (10-30 jig kg-1) was ineffective on the urinary bladder though it increased the systemic blood pressure. This latter effect was blocked by the x-receptor blocking agent phentolamine (2 mg kg-1).3 Acetylcholine (2-8ytg kg-1) caused a marked fall in systemic blood pressure, which was potentiated by physostigmine, but failed to produce any response on the intravesicular pressure even after physostigmine (50-100 ig kg-') treatment.4 ATP (2 mg kg-) produced an increase in intravesicular pressure accompanied by a fall in systemic blood pressure. The increased intravesicular pressure was antagonized by quinidine (20 mg kg-'); however, the fall in blood pressure remained unaltered. (50-100pgkg-1) was not affected by phentolamine (2mgkg-1), propranolol (1 mgkg-') or guanethidine (15-20 mg kg-1). l lgkg-'), hemicholinium 3 (2 mgkg-1) or atropine (1 mgkg-') were also unable to affect the response to nicotine. 6 Hexamethonium (1 mg kg-'), reduced the amplitude of spontaneous bladder contractions and quinidine (20 mg kg-') abolished the effect of nicotine.

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Section: Discussionmentioning

confidence: 99%

The effects of nicotine on spontaneous contractions of cat urinary bladder in situ

Koley

Saha

1984

British J Pharmacology

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“…This nerve is predominantly sympathetic, but may also contain parasympathetic elements (see [430]). When the hypogastric nerve is stimulated in the cat it causes the bladder to contract; this contraction is reduced by ANAPP 3 [663,664,670], implying that ATP is being released. Furthermore, 6-hydroxydopamine, which destroys sympathetic nerves, prevents this contractile response, indicating that the ATP is released from sympathetic nerves [665].…”

Section: Sympathetic Cotransmissionmentioning

confidence: 99%

Purinergic signalling in the urinary tract in health and disease

Burnstock

2013

Purinergic Signalling

143

149

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Purinergic signalling is involved in a number of physiological and pathophysiological activities in the lower urinary tract. In the bladder of laboratory animals there is parasympathetic excitatory cotransmission with the purinergic and cholinergic components being approximately equal, acting via P2X1 and muscarinic receptors, respectively. Purinergic mechanosensory transduction occurs where ATP, released from urothelial cells during distension of bladder and ureter, acts on P2X3 and P2X2/3 receptors on suburothelial sensory nerves to initiate the voiding reflex, via low threshold fibres, and nociception, via high threshold fibres. In human bladder t h e p u r i n e rg i c c o m p o n e n t o f p a r a s y m p a t h e t i c cotransmission is less than 3 %, but in pathological conditions, such as interstitial cystitis, obstructed and neuropathic bladder, the purinergic component is increased to 40 %. Other pathological conditions of the bladder have been shown to involve purinoceptor-mediated activities, including multiple sclerosis, ischaemia, diabetes, cancer and bacterial infections. In the ureter, P2X7 receptors have been implicated in inflammation and fibrosis. Purinergic therapeutic strategies are being explored that hopefully will be developed and bring benefit and relief to many patients with urinary tract disorders.

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“…Previous studies of the mechanical responses of guinea-pig bladder muscle have suggested that co-transmission in this structure may involve acetylcholine and a non-cholinergic non-adrenergic, possibly purinergic, transmitter (Mackenzie et al, 1982;Theobald, 1982;Moss & Burnstock, 1985).…”

Section: Introductionmentioning

confidence: 99%

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

Brading

Mostwin

1989

British J Pharmacology

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1 The electrical and mechanical responses to transmural stimulation of intrinsic nerves have been recorded from smooth muscle strips dissected from the dome of the guinea-pig bladder, by use of intracellular microelectrodes, and conventional tension recording techniques. 2 Stimulation of intrinsic nerves evoked action potentials in all cells studied. Hyperpolarization of the cells by extracellular current injection revealed subthreshold excitatory junction potentials (ej.ps) in about a quarter of the cells studied. 3 Action potentials could still be evoked in the presence of atropine and neostigmine, but were abolished after desensitization of the cells to a,#-methylene ATP, a stable analogue of ATP.4 In the presence of neostigmine, the evoked action potential was followed by a slow depolarization of the membrane. The mechanical response increased in amplitude and duration. 5 The contractile response to transmural nerve stimulation was reduced but not abolished in the presence of either atropine or desensitizing doses of a,fi-methylene ATP. Atropine was more effective at high frequencies of stimulation () 30Hz), and a,#-methylene ATP at low frequencies ( < 15 Hz). In combination the drugs abolished the response. 6 The results suggest that the mechanical response to excitatory nerve stimulation is biphasic. The early transient reponse is elicited by ej.ps and evoked spikes, is resistant to atropine, but sensitive to desensitization of purinoceptors. The late response is mediated through muscarinic receptors, involves little membrane depolarization, and is unaffected by desensitization of purinoceptors. These responses are analogous to the responses seen in rabbit bladder, and in the sympathetically innervated rat tail artery and guinea-pig vas deferens.

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ARYLAZIDO AMINOPROPIONYL ATP (ANAPP₃) ANTAGONISM OF CAT URINARY BLADDER CONTRACTIONS

Cited by 36 publications

References 6 publications

The effects of nicotine on spontaneous contractions of cat urinary bladder in situ

The effects of nicotine on spontaneous contractions of cat urinary bladder in situ

Purinergic signalling in the urinary tract in health and disease

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

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ARYLAZIDO AMINOPROPIONYL ATP (ANAPP3) ANTAGONISM OF CAT URINARY BLADDER CONTRACTIONS

Cited by 36 publications

References 6 publications

The effects of nicotine on spontaneous contractions of cat urinary bladder in situ

The effects of nicotine on spontaneous contractions of cat urinary bladder in situ

Purinergic signalling in the urinary tract in health and disease

Electrical and mechanical responses of guinea‐pig bladder muscle to nerve stimulation

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Product

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ARYLAZIDO AMINOPROPIONYL ATP (ANAPP₃) ANTAGONISM OF CAT URINARY BLADDER CONTRACTIONS