Arylsulphatase A activity and sulphatide concentration in the female rabbit oviduct are under physiological hormonal influence

Abstract

Oviduct samples of female rabbits in different phases of the reproductive cycle were analysed to establish the role of sex steroid hormones in the regulation of sulphatide concentration and arylsulphatase A activity. In addition to biochemical procedures, histochemical techniques were used to localize both enzyme activity and the natural substrate. The plasma concentrations of progesterone and 17 beta-oestradiol were determined by radioimmunoassay (RIA). The findings show that the parameters examined undergo considerable changes during the different phases of the reproductive cycle. Oestrogens exert an inducing action on arylsulphatase A activity, while progesterone inhibits it. Fluctuations of the catabolic arylsulphatase activity condition the sulphatide concentration, which reaches maximum values at the eighth post-ovulatory day when progesterone dominance is consolidated. In this phase of the reproductive cycle, thin-layer chromatography confirms the presence not only of larger quantities of sulphatides, but also of all other lipid fractions.

Steroid sulfatase, arylsulfatases A and B, galactose-6-sulfatase, and iduronate sulfatase in mammary cells and effects of sulfated and non-sulfated estrogens on sulfatase activity

“…In contrast, ASB activity decreased during the follicular phase and increased during the luteal phase. The level of ASA activity was greater than that of ASB [10][11][12].…”

Oviduct: roles in fertilization and early embryo development

“…Heat shock protein member A2 (HSPA2) is present in the human spermatozoa and binds with arylsulfatase A (ARSA) and sperm adhesion molecule 1 (SPAM1) (Redgrove et al 2012, 2013, Bromfield et al 2016. Both ARSA and SPAM1 are detected in the rabbit and mouse oviduct (Vitaioli et al 1996, Griffiths et al 2008. HSPA2 can also bind with angiotensin-converting enzyme (ACE) and protein disulfide isomerase A6 (PDIA6) to form a complex, and then engage in sperm-zona recognition.…”

Can endometrial arylsulfatase A activity predict the onset of endometrial polyps over the years?

“…We were unable to check the steroid receptors expression in the endometrial specimens. However, previous data widely suggested that ASA activity is enhanced by progesterone 5,6,22,23 .…”

“…Many genes are expressed in the endometrium during the menstrual cycle in relation to hormonal stimulation, with the goal to prepare the endometrium for implantation 4 . Arylsulphatase A (ASA) expression is regulated by estrogens and progesterone, because estrogens decrease ASA activity and progesterone increases it [5][6][7][8] . ASA is a lysosomal enzyme that catabolizes sulphatides (SL).…”