As-hyperaccumulator Pteris vittata and non-hyperaccumulator Pteris ensiformis under low As-exposure: Transcriptome analysis and implication for As hyperaccumulation

Sun, Dan; Zhang, Xiang; Yin, Zeyu; Feng, Huayuan; Hu, Chunyan; Guo, Nan; Tang, Yetao; Qiu, Rongliang; Lena, Q.; Cao, Yue

doi:10.1016/j.jhazmat.2023.132034

Cited by 12 publications

(22 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although As exposure induces the gene expression of P transporters, AsV reductases and AsIII antiporters in both plants, PvACR3;1/3;3 localized at vacuolar membrane are only present in P. vittata, which enhances its efficacy in AsIII sequestration into the frond vacuoles compared to P. ensiformis. 17 Compared to the 200 μM P control, Fe-phytate upregulated PvACR3;1 expression by 4.3-fold while Ca-phytate upregulated PvACR3;3 expression by 2.4-fold in P. vittata fronds (Figure 4E,F). The results suggest that PvACR3;3 seems to be more efficient in AsIII sequestration than PvACR3;1.…”

Section: ■ Results and Discussionmentioning

confidence: 89%

“…50,51 Vacuolar sequestration is a critical step for As accumulation in P. vittata, distinguishing it from typical plants. Sun et al 17 indicated that the As metabolism in P. vittata fronds is unique compared to non-hyperaccumulator P. ensiformis. Although As exposure induces the gene expression of P transporters, AsV reductases and AsIII antiporters in both plants, PvACR3;1/3;3 localized at vacuolar membrane are only present in P. vittata, which enhances its efficacy in AsIII sequestration into the frond vacuoles compared to P. ensiformis.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…As a structural analogue of P, AsV uptake by plants is through Pht1 transporters including P. vittata. 17 Currently, five P transporters in Pht1 family, including Pht1;1−4/6, have been identified, which are related to P and/or As accumulation in P. vittata. 18,20 Although they are all expressed in P. vittata, their responses to P and/or As exposure differ.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…After being taken up by P. vittata roots, AsV is reduced to arsenite (AsIII) by AsV reductases, with PvHAC1 being expressed primarily in P. vittata roots and PvHAC2 in the fronds. 17 Additionally, there are four AsIII-antiporters ACR3 in P. vittata, which contribute to its ability to efficiently metabolize excess As. 20 Specifically, PvACR3/3;2 in the roots are responsible for AsIII translocation to the fronds, while PvACR3;1/3;3 in the fronds are involved in sequestering AsIII into frond vacuoles.…”

Section: ■ Introductionmentioning

confidence: 99%

“…vittata roots, AsV is reduced to arsenite (AsIII) by AsV reductases, with PvHAC1 being expressed primarily in P. vittata roots and PvHAC2 in the fronds . Additionally, there are four AsIII-antiporters ACR3 in P.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Insoluble-Phytate Improves Plant Growth and Arsenic Accumulation in As-Hyperaccumulator Pteris vittata: Phytase Activity, Nutrient Uptake, and As-Metabolism

Liu,

Hu,

Xiao

et al. 2024

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Phytate, the principal P storage in plant seeds, is also an important organic P in soils, but it is unavailable for plant uptake. However, the As-hyperaccumulator Pteris vittata can effectively utilize soluble Na-phytate, while its ability to utilize insoluble Ca/Fe-phytate is unclear. Here, we investigated phytate uptake and the underlying mechanisms based on the phytase activity, nutrient uptake, and expression of genes involved in As metabolisms. P. vittata plants were cultivated hydroponically in 0.2strength Hoagland nutrient solution containing 50 μM As and 0.2 mM Na/Ca/Fe-phytate, with 0.2 mM soluble-P as the control. As the sole P source, all three phytates supported P. vittata growth, with its biomass being 3.2−4.1 g plant −1 and Ca/Fe-phytate being 19−29% more effective than Na-phytate. Phytate supplied soluble P to P. vittata probably via phytase hydrolysis, which was supported by 0.4−0.7 nmol P min −1 g −1 root fresh weight day −1 phytase activity in its root exudates, with 29−545 μM phytate-P being released into the growth media. Besides, compared to Na-phytate, Ca/ Fe-phytate enhanced the As contents by 102−140% to 657−781 mg kg −1 in P. vittata roots and by 43−86% to 1109−1447 mg kg −1 in the fronds, which was accompanied by 21−108% increase in Ca and Fe uptake. The increased plant As is probably attributed to 1.3−2.6 fold upregulation of P transporters PvPht1;3/4 for root As uptake, and 1.8−4.3 fold upregulation of arsenite antiporters PvACR3/3;1/3;3 for As translocation to and As sequestration into the fronds. This is the first report to show that, besides soluble Na-phytate, P. vittata can also effectively utilize insoluble Ca/Fe-phytate as the sole P source, which sheds light onto improving its application in phytoremediation of As-contaminated sites.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 89%