AS1411 aptamer-functionalized exosomes in the targeted delivery of doxorubicin in fighting colorectal cancer

Hosseini, Nashmin Fayazi; Amini, Razieh; Ramezani, Mohammad; Saidijam, Massoud; Hashemi, Seyed Mahmoud; Najafi, Rezvan

doi:10.1016/j.biopha.2022.113690

Cited by 57 publications

(20 citation statements)

References 41 publications

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“…81 The study demonstrated an enhanced anticancer effect in nucleolin, overexpressing cancer cells when sEVs grafted with aptamer AS1411 via thiol-maleimide covalent conjugation with cholesterol (Chol-PEG2000-maleimide) and loaded with paclitaxel was tested as a therapeutic modality. Harnessing the nucleolin targeting potential of AS1411 HEK 293 cellderived exosomes loaded with doxorubicin were surface functionalized EDC/NHS amide coupling chemistry by Hosseini et al 82 This strategy gives another alternative idea of covalent conjugation of the surface protein of sEVs with aptamer by carbonyl bond. The complex yielded enhanced therapeutic potential in vitro and in vivo colorectal cancer models.…”

Section: Acs Biomaterialsmentioning

confidence: 99%

Physio-chemical Modifications to Re-engineer Small Extracellular Vesicles for Targeted Anticancer Therapeutics Delivery and Imaging

Asfiya,

Xu,

Paramanantham

et al. 2024

ACS Biomater. Sci. Eng.

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Cancer theranostics developed through nanoengineering applications are essential for targeted oncologic interventions in the new era of personalized and precision medicine. Recently, small extracellular vesicles (sEVs) have emerged as an attractive nanoengineering platform for tumordirected anticancer therapeutic delivery and imaging of malignant tumors. These natural nanoparticles have multiple advantages over synthetic nanoparticle-based delivery systems, such as intrinsic targeting ability, less immunogenicity, and a prolonged circulation time. Since the inception of sEVs as a viable replacement for liposomes (synthetic nanoparticles) as a drug delivery vehicle, many studies have attempted to further the therapeutic efficacy of sEVs. This article discusses engineering strategies for sEVs using physical and chemical methods to enhance their anticancer therapeutic delivery performance. We review physio-chemical techniques of effective therapeutic loading into sEV, sEV surface engineering for targeted entry of therapeutics, and its cancer environment sensitive release inside the cells/organ. Next, we also discuss the novel hybrid sEV systems developed by a combination of sEVs with lipid and metal nanoparticles to garner each component's benefits while overcoming their drawbacks. The article extensively analyzes multiple sEV labeling techniques developed and investigated for live tracking or imaging sEVs. Finally, we discuss the theranostic potential of engineered sEVs in future cancer care regimens.

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Section: Acs Biomaterialsmentioning

confidence: 99%

Physio-chemical Modifications to Re-engineer Small Extracellular Vesicles for Targeted Anticancer Therapeutics Delivery and Imaging

Asfiya,

Xu,

Paramanantham

et al. 2024

ACS Biomater. Sci. Eng.

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“…With the advantages of small size, homing ability, hypoimmunogenicity, and strong tolerance of physiological barriers, EVs have been designed as vehicles for delivering diagnostic or therapeutic agents, such as small molecule drugs, 142 proteins, 143 and RNAs. 94,[144][145][146][147] To improve the selectivity of drug delivery, aptamers targeting specific proteins on receptor cells can be functionalized on EVs loading therapeutic agents, such as DOX, 73,96 miRNA, 71 and siRNA. 147 For example, Zou et al assembled the diacyl lipid-sgc8 aptamer on exosomes loading DOX by hydrophobic interaction to form Apt-Exos particles (Figure 11A).…”

Section: In Drug Delivery and Treatment Of Diseasesmentioning

confidence: 99%

Aptamer‐based extracellular vesicle isolation, analysis and therapeutics

Zhu

Tian

Yuan³

et al. 2023

Interdisciplinary Medicine

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Extracellular vesicles (EVs) play an important role in many physiological processes. Thus, EV analysis has a great value for the understanding of mechanisms underlying disease progress or diagnosis, prognosis and therapy. The overlapped physical and immune properties between EVs and events in body fluids, as well as the phenotypic heterogeneity of EVs, require efficient isolation and analysis methods. The unique properties of aptamers, such as facile modification and programmability, make them easily assembled as powerful platforms for EV isolation and analysis. EVs can also be used as vehicles for drug delivery, benefiting from the properties of homing ability, hypo-immunogenicity, and strong tolerance. The affinity recognition ability to targets and the feature of single stranded DNA of aptamers make them useful in promoting the targetability of EVs and delivery of nucleic acid drugs. This review summarizes recent progress in aptamer-based EV isolation, analysis, and aptamer-functionalized EVs for therapeutics. K E Y W O R D Saptamer, drug delivery, extracellular vesicles, extracellular vesicle detection, extracellular vesicle isolation | INTRODUCTIONExtracellular vesicles (EVs) are phospholipid bilayerenclosed extracellular structures present in body fluids, such as blood, urine, saliva, and tears. 1,2 EVs are formed by outward budding of cell membranes (ectosomes) or the intracellular endocytic trafficking pathway involving the fusion of late endosomal multivesicular bodies with the cell membrane (exosomes). 3,4 Ectosomes, including microparticles, micro-vesicles, and large vesicles, are released from the plasma membrane with a size range of 50 nm to 1 μm and were found to be messengers to transfer functional proteins and nucleic acids. [5][6][7] In addition, exosomes are small vesicles secreted from all types of cells This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…This study showed that 200 μg exosomes were incubated with 300 μg/mL DOX to obtain the best loading capacity with an encapsulation efficiency close to 13%. Compared with free DOX, DOX-Apt-Exo improved the target homing ability and reduced the cytotoxicity of DOX . Passive encapsulation methods are more suitable for hydrophobic and small molecule cargoes, while for hydrophilic and macromolecular cargoes, stronger stimulation is required to encapsulate them into the exosomes …”

Section: Engineered Exosomesmentioning

confidence: 99%

“…Compared with free DOX, DOX-Apt-Exo improved the target homing ability and reduced the cytotoxicity of DOX. 46 Passive encapsulation methods are more suitable for hydrophobic and small molecule cargoes, while for hydrophilic and macromolecular cargoes, stronger stimulation is required to encapsulate them into the exosomes. 47 Active encapsulation uses mechanical techniques to transiently and reversibly open exosomal membranes to allow cargos to enter the exosomes, with common methods such as sonication, electroporation, freeze−thaw cycles, coextrusion, and surfactant.…”

Section: Engineered Exosomesmentioning

confidence: 99%

Research Advances of Engineered Exosomes as Drug Delivery Carrier

Huang,

Wu,

Liao

et al. 2023

ACS Omega

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Exosomes are nanoscale vesicles secreted by living cells that have similar membrane composition to parental cells and carry a variety of proteins, lipids, and nucleic acids. Therefore, exosomes have certain biological activities and play an important role in intercellular communication. On the basis of its potential as a carrier for drug delivery systems, exosomes have been engineered to compensate for the shortage of natural exosomes through various engineering strategies for improving drug delivery efficiency, enhancing targeting to tissues and organs, and extending the circulating half-life of exosomes. This review focuses on the engineered exosomes loading drugs through different strategies, discussions on exosome surface modification strategies, and summarizes the advantages and disadvantages of different strategies. In addition, this review provides an overview of the recent applications of engineered exosomes in a number of refractory and relapsable diseases. This review has the potential to provide a reference for further research and development of engineered exosomes.

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AS1411 aptamer-functionalized exosomes in the targeted delivery of doxorubicin in fighting colorectal cancer

Cited by 57 publications

References 41 publications

Physio-chemical Modifications to Re-engineer Small Extracellular Vesicles for Targeted Anticancer Therapeutics Delivery and Imaging

Physio-chemical Modifications to Re-engineer Small Extracellular Vesicles for Targeted Anticancer Therapeutics Delivery and Imaging

Aptamer‐based extracellular vesicle isolation, analysis and therapeutics

Research Advances of Engineered Exosomes as Drug Delivery Carrier

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