Ascaris lumbricoides cystatin induces specific IgE but not allergic response

Sandra, Coronado; Manotas, María Carolina; Josefina, Zakzuk; Caraballo, Luis

doi:10.3389/conf.fimmu.2015.05.00295

Cited by 4 publications

(4 citation statements)

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“…The nasal challenge with rAl-CPI did not induce an eosinophilic inflammatory response in airways, neither increased Penh values as observed with the B tropicalis extract. Also, rAl-CPI specific IgE levels were undetectable in sensitized/challenged mice perhaps justifying the failure of rAl-CPI to increase airway reactivity (supported by the Penh values) and induce passive cutaneous anaphylaxis, in our previous work (76). Concerns about the allergenic potential of cystatins are raised by the recognition of the Anisakis simplex cystatin as an allergen (Ani s 4) (77) as well as others reported in kiwi and cat (78, 79).…”

Section: Discussionsupporting

confidence: 52%

Ascaris lumbricoides Cystatin Prevents Development of Allergic Airway Inflammation in a Mouse Model

et al. 2019

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Severe helminth infections are negatively associated to allergic diseases like asthma; therefore, the immunomodulatory properties of parasite-derived components have been analyzed, raising the possibility of their use as anti-inflammatory molecules. We evaluated the immunomodulatory properties of Ascaris lumbricoides recombinant cysteine protease inhibitor (rAl-CPI) in a mouse model of allergic airway inflammation induced by the house dust mite (HDM) Blomia tropicalis and its effects on human monocyte-derived dendritic cells (HmoDCs). The B. tropicalis sensitized/challenged mice developed extensive cellular airway inflammatory response, which was significantly reduced upon treatment with rAl-CPI prior to B. tropicalis sensitization, affecting particularly the perivascular/peribronchial infiltrate cells, eosinophils/neutrophils, and goblet cells. A significant decrease of Th2 cytokines, total, and specific IgE antibodies was observed in rAl-CPI treated mice. The antibody response was biased to IgG, mainly IgG2a. Administration of rAl-CPI-alone and rAl-CPI before mite sensitization were associated with a significant increase of regulatory T cells (Tregs) in spleen and elevated IL-10 levels in BAL and splenocytes culture supernatants, which was partially affected by anti-IL10 receptor use. In vitro, rAl-CPI showed a modulatory effect on HmoDCs, lowering the expression of HLA-DR, CD83, and CD86, while inducing IL-10 and IL-6 production. This suggests an inhibition of HmoDC maturation and a possible link with the inhibition of the allergic response observed in the murine model.

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Section: Discussionsupporting

confidence: 52%

Ascaris lumbricoides Cystatin Prevents Development of Allergic Airway Inflammation in a Mouse Model

et al. 2019

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“…In connection with this, an interesting information comes from studies that evaluate early patterns of IgG responses and IgE sensitization (ratio IgG/IgE) to mite and other allergens, which suggest the existence of protection from allergy symptoms in individuals with the highest IgG/IgE ratios [11][12][13]. These findings are similar to those found during chronic helminth infections, where IgG response is stronger than that of IgE and allergy symptoms are almost absent; in fact, we have discovered parasite components that bind IgE with high frequency but are not allergenic [14] and this could occur to mite IgE-binding components whose allergenic properties have not yet been fully tested.…”

Section: The Allergenicity Of Mite Extracts and Their Ige-binding Comsupporting

confidence: 76%

Mite allergens

Caraballo

2017

Expert Review of Clinical Immunology

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“…rAl-CPI also induced the production of IL-10 and TGFB by macrophages, 113 and further work in an animal model of airway inflammation has confirmed its IL-10-associated antiinflammatory activity showing the induction of regulatory T cells as well as the production of IL-10 by human monocyte-derived dendritic cells. 115 In addition, since some helminth products could be potential inducers of adverse allergic reactions, several tests for allergenic activity have been done on rAl-CPI, finding that it binds IgE but does not have allergenic activity 116 ; therefore, this A. lumbricoides-derived immunomodulator seems promising for treating immune-mediated inflammation of intestine and lung.…”

Section: Whethermentioning

confidence: 99%

Ascariasis as a model to study the helminth/allergy relationships

Caraballo

Acevedo

Zakzuk

2018

Parasite Immunology

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Summary Ascariasis is the most frequent soil transmitted helminthiasis and, as well as other helminth infections, is expected to influence the clinical presentation of allergic diseases such as asthma. Indeed, several clinical and experimental works have shown an important impact either increasing or suppressing symptoms, and the same effects have been detected on the underlying immune responses. In this review we analyze the work on this field performed in Colombia, a Latin American tropical country, including aspects such as the molecular genetics of the IgE response to Ascaris; the allergenic activity of Ascaris IgE‐binding molecular components and the immunological and clinical influences of ascariasis on asthma. The analysis allows us to conclude that the impact of ascariasis on the inception and evolution of allergic diseases such as asthma deserves more investigation, but advances have been made during the last years. The concurrent parasite‐induced immunostimulatory and immunosuppressive effects during this helminthiasis do modify the natural history of asthma and some aspects of the practice of allergology in the tropics. Theoretically it can also influence the epidemiological trends of allergic diseases either by its absence or presence in different regions and countries.

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Ascaris lumbricoides cystatin induces specific IgE but not allergic response

Cited by 4 publications

References 0 publications

Ascaris lumbricoides Cystatin Prevents Development of Allergic Airway Inflammation in a Mouse Model

Ascaris lumbricoides Cystatin Prevents Development of Allergic Airway Inflammation in a Mouse Model

Mite allergens

Ascariasis as a model to study the helminth/allergy relationships

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