Ascaris suum: Immunoglobulin responses in mice

Crandall, Catherine A.; Crandall, Richard B.

doi:10.1016/0014-4894(71)90107-x

Cited by 55 publications

(22 citation statements)

References 30 publications

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“…In addition, crude larval antigens can induce protective immunity (61). Similar findings were observed in an A. suum laboratory animal infection model (8,22,49). Passive transfer of sera from immune animals is effective for larval killing and stunting in guinea pigs (27).…”

mentioning

confidence: 64%

“…IPTG (isopropyl-␤-D-thiogalactopyranoside) was then added to a final concentration of 1 mM, and the culture was grown for an additional 4 h at 37°C. The E. coli cells were pelleted and resuspended in a lysis buffer (50 mM NaH 2 PO 4 [pH 8.0], 10 mM Tris-HCl [pH 8.0], 100 mM NaCl). Lysozyme was added to 100 g/ml, and the cell suspension was incubated on ice for 20 min.…”

Section: Methodsmentioning

confidence: 99%

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Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

et al. 2003

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Protective immunity to the pig roundworm, Ascaris suum, has been demonstrated by immunization of pigs with antigens derived from the parasite's larval stages. We identified a protective antigen commonly expressed in the human and pig Ascaris infections as a 16-kDa protein (As16), which has no similarity at the amino acid level to mammalian proteins but has some similarity to those of the filarial parasites and Caenorhabditis elegans gene product. Localization analysis revealed that the native As16 was highly expressed in the adult worm intestine, hypodermis, and cuticles. In addition, As16 was detected in the parasite excretory and secretory products. Mice intranasally vaccinated with Escherichia coli-expressed recombinant As16 (rAs16), coupled with cholera toxin B subunit, generated a significant increase in the level of rAs16-specific immunoglobulin G (IgG) and IgE in serum. Mucosal IgA levels were also increased. The recombinant protein evoked a mixed (both Th1 and Th2) type of immune response characterized by elevated levels of gamma interferon and interleukin-10 in the culture supernatants of activated spleen cells. An increased level of IgG1 and IgG2a in serum was also observed. The vaccinated mice showed a reduction by 58% in the recovery of challenged larvae compared to a nonvaccinated control. These results suggest the possibility of developing a mucosal vaccine for human and pig ascariasis.

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confidence: 64%

Section: Methodsmentioning

confidence: 99%

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

et al. 2003

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“…Hypergammaglobulinaemia is a common feature of parasitic infection (Cohen, 1974) and increased levels of IgM (Crandall and Crandall, 1971) and the two mast cell-binding isotypes of mice, i.e. heat-labile reagins (IgE) (Jarrett, 1973) and IgG, (Crandall, Crandall and Franco, 1974;Sher, Mclntyre and von Lichtenberg, 1977;Mitchell et al, 1977a;Prowso, Ey and Jenkin, 1978a) have been described in murine metazoan parasitic infections.…”

Section: Introductionmentioning

confidence: 99%

IgG₁ HYPERGAMMAGLOBULINAEMIA IN CHRONIC PARASITIC INFECTIONS IN MICE: MAGNITUDE OF THE RESPONSE IN MICE INFECTED WITH VARIOUS PARASITES

Chapman

Knopf

Hicks

et al. 1979

Aust J Exp Biol Med

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Summary Mice chronically infected with 3 metazoan and 1 protozoan parasite contain in their circulation levels of IgG1 which are increased over the levels in uninfected mice by at least 10×. In the case of infection with the larval cestode, Mesocestoides corti. the serum IgG1 concentration can reach > 50 mg/ml and, with a half‐life of < 2 days, the number of cells engaged in IgG1 production is approximately 2 × 108. The IgG1 hypergammaglobulinaemia is not seen in infected hypothymic nude mice. Biosynthetic labelling studies with organ and tissue cultures established that in two of the chronic infections the organs principally involved in IgG1 synthesis were those pathologically involved or those “in line” for antigen capture: i.e. liver and spleen in the case of M. corti which is located in the liver and the peritoneal cavity, and various intestinal lymph nodes in the case of the gut‐dwelling nematode, Nematospiroides dubius. This apparently exaggerated response to chronic parasitic infection is of interest simply because of the potential magnitude of the effect and the fact that it involves an Ig isotype with very poorly defined biological function.

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“…Since A. suum embryonated eggs can hatch and their larvae can migrate into a wide range of hosts, experimental animal-A. suum infection models have been used for immunological and chemotherapeutic experiments (10,24,25,45).…”

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Intranasal Immunization with RecombinantAscaris suum14-Kilodalton Antigen Coupled with Cholera Toxin B Subunit Induces Protective Immunity toA. suumInfection in Mice

et al. 2001

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Animals can be rendered immune to Ascaris parasites by immunization with infectious-stage larvae. The specific parasite gene products that mediate protective responses in ascariasis are unknown. We have identified a cDNA encoding Ascaris suum 14-kDa antigen (As14) and evaluated the vaccinal effect of the Escherichia coli-expressed recombinant protein (rAs14). GenBank analysis showed that As14 has low similarity at the amino acid level to a Caenorhabditis elegans gene product and to antigens of the filarial nematodes but not to other known proteins. In addition, As14 homologues were found to be expressed in human and dog roundworms. In mice that received intranasal administration of rAs14 coupled with cholera toxin B subunit (rAs14-CTB), there was a 64% reduction of recovery of larvae compared with that in the nontreated group. The vaccinated mice showed a significant increase in the total serum immunoglobulin G (IgG) levels and the mucosal IgA responses. Elevation of the rAs14-specific IgE response was also seen. Measurement of the IgG subclasses showed a higher level of IgG1 and a lower level of IgG2a antibody response in the sera of the immunized mice, suggesting that protection was associated with a type II immune response. As14 is the first protective antigen against A. suum infection to be identified. Our immunization trial results in laboratory animals suggest the possibility of developing a mucosal vaccine for parasitic diseases caused by ascarid nematodes.

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Ascaris suum: Immunoglobulin responses in mice

Cited by 55 publications

References 30 publications

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

IgG₁ HYPERGAMMAGLOBULINAEMIA IN CHRONIC PARASITIC INFECTIONS IN MICE: MAGNITUDE OF THE RESPONSE IN MICE INFECTED WITH VARIOUS PARASITES

Intranasal Immunization with RecombinantAscaris suum14-Kilodalton Antigen Coupled with Cholera Toxin B Subunit Induces Protective Immunity toA. suumInfection in Mice

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Ascaris suum: Immunoglobulin responses in mice

Cited by 55 publications

References 30 publications

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

IgG1 HYPERGAMMAGLOBULINAEMIA IN CHRONIC PARASITIC INFECTIONS IN MICE: MAGNITUDE OF THE RESPONSE IN MICE INFECTED WITH VARIOUS PARASITES

Intranasal Immunization with RecombinantAscaris suum14-Kilodalton Antigen Coupled with Cholera Toxin B Subunit Induces Protective Immunity toA. suumInfection in Mice

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IgG₁ HYPERGAMMAGLOBULINAEMIA IN CHRONIC PARASITIC INFECTIONS IN MICE: MAGNITUDE OF THE RESPONSE IN MICE INFECTED WITH VARIOUS PARASITES