Ascending dopaminergic projections from the dorsal raphe nucleus in the rat

Stratford, Thomas R.; Wirtshafter, David

doi:10.1016/0006-8993(90)90239-8

Cited by 119 publications

(72 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, Gras and co-workers reported that numerous neurons in the dorsal raphe were VGLUT3-ir, but not serotonergic. Thus, a second possibility is a nonserotonergic glutamatergic projection from the DR to the LS (Köhler et al, 1982;Stratford and Wirtshafter, 1990). Shutoh et al (2008) recently found VGLUT3-ir fibers in the LSD that were immunoreactive for serotonin in adult male Sprague-Dawley rats implicating that there possibly exist strain differences.…”

Section: Coincidence Of Vglut3-ir With Markers That Define Major Neurmentioning

confidence: 99%

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Riedel

Westerholz

Braun

et al. 2008

Journal of Chemical Neuroanatomy

View full text Add to dashboard Cite

The lateral septum (LS) plays a role in the adjustment of behavioral responses according to environmental demands. This is a complex integrative process wherein a variety of modulatory systems, i.e. cholinergic, dopaminergic and serotonergic projections forming pericellular baskets around LS neurons, are involved.Recently, vesicular glutamate transporter 3 (VGLUT3)-immunoreactive (-ir) structures outlining unlabeled somata and their proximal dendrites were described in the LS. However, the vesicular transporters for acetylcholine and GABA were not or only rarely co-expressed with VGLUT3.In this study, the morphology and distribution of these VGLUT3-ir structures were systematically analyzed revealing that (1) they form distinct pericellular baskets (PBs) displaying variable shapes, (2) they are arranged in a layer-like pattern similar to the terminals of other modulatory systems, (3) beside a few exceptions (e.g., choline acetyltransferase), they are generally not or very sparsely colocalized with other neurochemical markers characterizing major neuron populations or afferent systems of the LS, i.e. calcium-binding proteins, tyrosine hydroxylase, tryptophan hydroxylase, vesicular glutamate transporters 1 (VGLUT1) and 2 (VGLUT2) and the vesicular GABA transporter.Thus, in the LS, a separate population of neurons is covered by VGLUT3-ir PBs. The distribution pattern and the lack of co-localization indicate that the VGLUT3-expressing cells of origin are located in the brainstem and that they could be pure glutamatergic projection neurons-different from the well-defined canonical VGLUT1-and VGLUT2-expressing neurons. Alternatively, they could

show abstract

Section: Coincidence Of Vglut3-ir With Markers That Define Major Neurmentioning

confidence: 99%

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Riedel

Westerholz

Braun

et al. 2008

Journal of Chemical Neuroanatomy

View full text Add to dashboard Cite

show abstract

Section: Nih Public Accessmentioning

confidence: 99%

“…Anterograde tracing studies with Phaseolus vulgaris leucoagglutinin (PHA-L) or biotinylated dextran amine (BDA) have demonstrated that regions of the basal forebrain are heavily targeted by DRN projections (Vertes, 1991;Sim and Joseph, 1993;Morin and Meyer-Bernstein, 1999), and retrograde tracing has confirmed that this projection arises, in part, from nonserotonergic neurons (Descarries et al, 1986;Semba et al, 1988;Stratford and Wirtshafter, 1990;Li et al, 1990; Van Bockstaele et al, 1993;Petrov et al, 1994;Ford et al, 1995;Gasbarri et al, 1999; Hansue and Shammah-Lagnado, 2002;Halberstadt and Balaban, 2006a). A recent study combined BDA anterograde tracing with 5-HT-immunofluorescent staining to demonstrate that the vast majority of DRN fibers innervating the septum are nonserotonergic (Aznar et al, 2004).…”

Section: Nih Public Accessmentioning

confidence: 99%

“…Experiments combining retrograde tracing with serotonin (5-HT) immunostaining have established that many nonserotonergic DRN neurons project extrinsically (Beitz et al, 1986;Ma et al, 1991;Petrov et al, 1992Petrov et al, , 1994 Van Bockstaele et al, 1993;Kirifides et al, 2001;Kim et al, 2004;Halberstadt and Balaban, 2006a). Nonserotonergic DRN projections are neurochemically heterogeneous, and may contain a number of transmitter substances, including glutamate (Schwarz and Schwarz, 1992;Kiss et al, 2002), dopamine (Pohle et al, 1984;Descarries et al, 1986;Yoshida et al, 1989;Stratford and Wirtshafter, 1990;Hasue and Shammah-Lagnado, 2002), GABA (Nanopoulos et al, 1982;Stamp and Semba, 1995;Ford et al, 1995;Jankowski and Sesack, 2002), nitric oxide (Xu and Hokfelt, 1997;Simpson et al, 2003), and neuropeptides such as vasoactive intestinal polypeptide (VIP) (Petit et al, 1995;Kozicz et al, 1998) and cholecystokinin Seroogy and Fallon, 1989).Although it is likely that non-5-HT DRN projections influence processing in target regions (Weiss and Pellet, 1982;Hajés-Korcsok and Sharp, 2002), very little is known about the anatomical organization of this pathway. Anterograde tracing studies with Phaseolus vulgaris leucoagglutinin (PHA-L) or biotinylated dextran amine (BDA) have demonstrated that regions of the basal forebrain are heavily targeted by DRN projections (Vertes, 1991;Sim and Joseph, 1993;Morin and Meyer-Bernstein, 1999), and retrograde tracing has confirmed that this projection arises, in part, from nonserotonergic neurons (Descarries et al, 1986;Semba et al, 1988;Stratford and Wirtshafter, 1990;…”

mentioning

confidence: 99%

“…Nonserotonergic DRN projections are neurochemically heterogeneous, and may contain a number of transmitter substances, including glutamate (Schwarz and Schwarz, 1992;Kiss et al, 2002), dopamine (Pohle et al, 1984;Descarries et al, 1986;Yoshida et al, 1989;Stratford and Wirtshafter, 1990;Hasue and Shammah-Lagnado, 2002), GABA (Nanopoulos et al, 1982;Stamp and Semba, 1995;Ford et al, 1995;Jankowski and Sesack, 2002), nitric oxide (Xu and Hokfelt, 1997;Simpson et al, 2003), and neuropeptides such as vasoactive intestinal polypeptide (VIP) (Petit et al, 1995;Kozicz et al, 1998) and cholecystokinin Seroogy and Fallon, 1989).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Selective anterograde tracing of nonserotonergic projections from dorsal raphe nucleus to the basal forebrain and extended amygdala

Halberstadt

Balaban

2008

Journal of Chemical Neuroanatomy

View full text Add to dashboard Cite

The dorsal raphe nucleus (DRN) contains both serotonergic and non-serotonergic projection neurons. Retrograde tracing studies have demonstrated that components of the basal forebrain and extended amygdala are targeted heavily by input from nonserotonergic DRN neurons. The object of this investigation was to examine the terminal distribution of nonserotonergic DRN projections in the basal forebrain and extended amygdala, using a technique that allows selective anterograde tracing of nonserotonergic DRN projections. To trace nonserotonergic DRN projections, animals were pretreated with nomifensine, desipramine and the serotonergic neurotoxin 5,7-dihydroxytryptamine (5,7-DHT), 7 days prior to placing an iontophoretic injection of biotinylated dextran amine (BDA) into the DRN. In animals treated with 5,7-DHT, numerous nonserotonergic BDA-labeled fibers ascended to the basal forebrain in the medial forebrain bundle system. Some of these labeled fibers crossed through the lateral hypothalamus, bed nucleus of the stria terminalis, and substantial innominata. These fibers entered the amygdala through the ansa peduncularis and ramified within the central and basolateral amygdaloid nuclei. Other fibers entered the diagonal band of Broca and formed a dense plexus of labeled fibers in the dorsal half of the intermediate portion of the lateral septal nucleus and the septohippocampal nucleus. These findings demonstrate that the basal forebrain and extended amygdala receive a dense projection from nonserotonergic DRN neurons. Given that the basal forebrain plays a critical role in processes such as motivation, affect, and behavioral control, these findings support the hypothesis that nonserotonergic DRN projections may exert substantial modulatory control over emotional and motivational functions. Keywords amygdala; bed nucleus; 5,7-dihydroxytryptamine; septal nuclei; biotinylated dextran amine; tyrosine hydroxylase It is well known that the dorsal raphe nucleus contains more than half of all the serotonergic neurons in the brain (Leger and Wiklund, 1982) and is a source of projections that terminate extensively throughout the central nervous system (Vertes, 1991;Sim and Joseph, 1993;Vertes and Kocsis, 1994;Morin and Meyer-Bernstein, 1999 Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. within dorsomedial (DRNdm), ventromedial (DRNvm), and lateral (DRNl) cell groups . It has been proposed that the activity of serotonergic DRN neurons plays an important role in the coordination of sensory processing and motor output with the sleep-wake-arousal cycle (Jacobs and Azmitia, 1992; Forn...

show abstract

Topographic organization and neurochemical identity of dorsal raphe neurons that project to the trigeminal somatosensory pathway in the rat

Kirifides

Simpson

Lin

et al. 2001

J of Comparative Neurology

View full text Add to dashboard Cite

The primary goals of this study were to: 1) examine the distribution of neurons within the dorsal raphe (DR) nucleus that project to cortical and subcortical sites along the trigeminal somatosensory pathway in rat; 2) determine the extent to which different regions within this ascending sensory system receive collateral projections from the same DR neuron; and 3) identify the putative transmitters contained within these DR projection neurons. Long-Evans hooded rats received pressure injections of various combinations of retrograde fluorescent tracers; into the whisker-related regions of the primary somatosensory cortex (barrel field cortex [BC]), ventral posterior medial thalamus (VPM), and principal nucleus of the trigeminal complex (PrV). The distribution of retrogradely labeled neurons within the DR was examined by fluorescence microscopy. The major finding was that cortically projecting neurons were located within the midline regions of the rostral portion of the DR, whereas cells projecting to subcortical trigeminal somatosensory structures were distributed bilaterally in the lateral wing regions of the DR as well as in the midline portions of the nucleus. Single neurons that send axon collaterals to multiple cortical and subcortical trigeminal somatosensory targets were observed in the dorsomedian and ventromedian regions of the DR. DR neurons that projected to cortical and subcortical sites contained serotonin but not tyrosine hydroxylase, the marker enzyme for catecholamine transmitters. Taken together, these findings provide further evidence of neurochemical specificity and functional anatomical organization within the DR efferent projection system.

show abstract

Ascending dopaminergic projections from the dorsal raphe nucleus in the rat

Cited by 119 publications

References 11 publications

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Selective anterograde tracing of nonserotonergic projections from dorsal raphe nucleus to the basal forebrain and extended amygdala

Topographic organization and neurochemical identity of dorsal raphe neurons that project to the trigeminal somatosensory pathway in the rat

Contact Info

Product

Resources

About