Ascite neoplásica: efeito da solução aquosa de fenol, ácido acético e glicerina sobre o tumor ascítico de Ehrlich

Abstract

OBJETIVO: Verificar o efeito da solução composta por fenol, ácido acético e glicerina sobre o tumor ascítico de Ehrlich. MÉTODOS: Utilizou-se 283 camundongos divididos em 2 protocolos (animais portadores e não portadores de tumor) procedendo-se a injeção de 0,25 ml, 0,10 ml e 0,05 ml da solução teste e 0,25 ml de solução salina, o sacrifício foi realizado após 3 e 6 dias do tratamento, analisando, a seguir, a contagem diferencial de células presentes no líquido ascítico. RESULTADOS: Observou-se que nos animais portadores de tumor houve uma redução significante do número de células tumorais e aumento do número de células inflamatórias, nos animais sem tumor observou-se influxo de células inflamatórias para a cavidade peritoneal. CONCLUSÃO: A solução proposta causa, in vivo, a diminuição do número de células tumorais e aumento do número de células inflamatórias no líquido ascítico.

Effects of acetylsalicylic acid and acetic acid solutions on VX2 liver carcinoma in rabbits: in vivo analysis

“…In a previous study, we used a solution composed of acetic acid, glycerin and phenol in the treatment of Ehrlich ascites tumor cells. We observed that the in vitro solution destroyed tumor cells and increased the number of inflammatory cells 4 . Therefore, it seemed appropriate to test one or more of these components separately in the livers of animals with tumors.…”

“…In a study performed in 1996 4 , we analyzed the effects of a solution consisting of acetic acid, glycerin, phenol and distilled water on Ehrlich ascites tumor cells in vitro and in vivo and observed that the solution caused tumor cell death in vitro. Intraperitoneal injection of the solution in vivo reduced the number of tumor cells and increased the number of inflammatory cells in suspension in the acetic acid solution 4 . In light of this observed effect, we decided to analyze the possible effects of one or more of the solution components in isolation.…”

Citotoxicity and immune response induced by organopalladium(II) compounds in mice bearing Ehrlich ascites tumour

“…After 3 days of tumour development, the compounds [{Pd(dmba)} 2 (μ-NCS) 2 ] (1), [Pd(dmba) (NCS)(dppp)] (2) and cis-DDP were inoculated, according to literature methods. 18 Groups were named according to the compounds inoculated:…”

“…After 3 days of tumour development, the compounds [{Pd(dmba)} 2 (μ-NCS) 2 ] (1), [Pd(dmba) (NCS)(dppp)] (2) and cis-DDP were inoculated, according to literature methods. 18 Groups were named according to the compounds inoculated:Group 1 or G1: cis-DDP (1 mL, i.p., 10 mg kg -1 ); Group 2 or G2: [Pd(dmba)(NCS)(dppp)] (1 mL, i.p., 1 mmol L -1 ); Group 3 or G3: [{Pd(dmba)} 2 (μ-NCS) 2 ] (1 mL, i.p., 1mmol L -1 ); Group 4 or G4: Vehicle control (1mL, i.p., DMSO 0.3%); Group 5 or G5: Tumour control (1mL, i.p., PBS). …”

“…Differentiation of cells was made by May-Grünwald Giemsa (Reagen) with subsequent observation in an optical microscope under immersion lens (100X). 18 Cell viability by trypan blue exclusion 100.0 μL samples of the cell suspension were added to 100.0 μL of Trypan blue solution (1:10) and the samples were dropped into glass sheets and observed in a microscope under 40X lens. We have considered dead the cells which absorbed Trypan blue solution.…”