Ascorbate peroxidase: A novel antioxidant enzyme in insects

Mathews, M. Claravon; Summers, Clinton B.; Felton, Gary W.

doi:10.1002/(sici)1520-6327(1997)34:1<57::aid-arch5>3.0.co;2-t

Cited by 104 publications

(37 citation statements)

References 39 publications

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“…H 2 O 2 is subsequently scavenged by CAT, resulting in the production of water and molecular oxygen. Ascorbate peroxidase also scavenges H 2 O 2 , but activity is probably limited to the H 2 O 2 not scavenged by CAT (11).…”

contrasting

confidence: 49%

Antioxidant defense response in a galling insect

Mittapalli¹,

Neal²,

Shukle

2007

Proc. Natl. Acad. Sci. U.S.A.

114

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Herbivorous insect species are constantly challenged with reactive oxygen species (ROS) generated from endogenous and exogenous sources. ROS produced within insects because of stress and prooxidant allelochemicals produced by host plants in response to herbivory require a complex mode of antioxidant defense during insect/plant interactions. Some insect herbivores have a midgutbased defense against the suite of ROS encountered. Because the Hessian fly (Mayetiola destructor) is the major insect pest of wheat worldwide, and an emerging model for all gall midges, we investigated its antioxidant responses during interaction with its host plant. Quantitative data for two phospholipid glutathione peroxidases (MdesPHGPX-1 and MdesPHGPX-2), two catalases (MdesCAT-1 and MdesCAT-2), and two superoxide dismutases (MdesSOD-1 and MdesSOD-2) revealed high levels of all of the mRNAs in the midgut of larvae on susceptible wheat (compatible interaction). During development of the Hessian fly on susceptible wheat, a differential expression pattern was observed for all six genes. Analysis of larvae on resistant wheat (incompatible interaction) compared with larvae on susceptible wheat showed increased levels of mRNAs in larvae on resistant wheat for all of the antioxidant genes except MdesSOD-1 and MdesSOD-2. We postulate that the increased mRNA levels of MdesPHGPX-1, MdesPH-GPX-2, MdesCAT-1, and MdesCAT-2 reflect responses to ROS encountered by larvae while feeding on resistant wheat seedlings and/or ROS generated endogenously in larvae because of stress/ starvation. These results provide an opportunity to understand the cooperative antioxidant defense responses in the Hessian fly/ wheat interaction and may be applicable to other insect/plant interactions.Hessian fly ͉ insect/plant interaction ͉ reactive oxygen species ͉ wheat

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contrasting

confidence: 49%

Antioxidant defense response in a galling insect

Mittapalli¹,

Neal²,

Shukle

2007

Proc. Natl. Acad. Sci. U.S.A.

114

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“…Thus, ROS production in insects, including Lymantria dispar, is strongly controlled by enzymatic and nonenzymatic antioxidants (Felton and Summers, 1995;Mathews et al, 1997;Perić-Mataruga et al, 1997). The Lymantria dispar midgut is the second-largest organ in larvae and the major site for elimination of toxic effects of plant allelochemicals, food digestion, transport of nutrients, regulation of ion balance, etc.…”

Section: Introductioncontrasting

confidence: 45%

Ghrelin effects on midgut tissue antioxidative defense and glutathioneS-transferase activity in Lymantria dispar (Lepidoptera)

Perić-Mataruga¹,

Vlahović²,

Mrdaković³

et al. 2015

Turk J Biol

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The aim of the study was to examine changes in Cu-Zn superoxide dismutase (SOD), catalase (CAT), their gel electrophoresis profiles, glutathione reductase (GR) activity, amount of glutathione (GSH), and the activity of glutathione S-transferase (GST) -phase II biotransformation enzyme in the midgut tissue of gypsy moth (Lymantria dispar L. (Lepidoptera: Lymantriidae)) larvae after ghrelin treatment. Four subpicomolar injections of ghrelin (0.3 pmol) or physiological saline (control) were applied every 24 h. The SOD, CAT, GR, GST activity, and amount of GSH were higher in the ghrelin-treated group than in the control. Electrophoresis gel bands of SOD and CAT had higher area and density in the treated group. The effects of ghrelin on the antioxidative defense and GST activity in insects were detected for the first time. The results provided evidence for possible application of insects as simple model systems in future studies of the role of ghrelin in the antioxidative protection of complex organisms.

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“…The ascorbate-recycling system has been detected and studied in Lepidoptera larvae and other leafchewing insects (Mathews et al 1997;Summers & Felton 1993;Barbehenn et al 2001;Wang et al 2001;Krishnan & Kodrik 2006) but not in sucking-piercing insects, including aphids. Thus, the aim of this study was to compare ASA content and enzyme activities associated with ASA metabolism in tissues of two species of cereal aphids: the monophagous grain aphid, Sitobion avenae (F., 1794), and the oligophagous bird cherryoat aphid, Rhopalosiphum padi (L., 1758).…”

Section: Introductionmentioning

confidence: 49%

Antioxidant defense mechanisms of cereal aphids based on ascorbate and ascorbate peroxidase

Łukasik¹,

Goławska²,

Wójcicka³

2009

Biologia

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Effects of plant o-dihydroxyphenols on ascorbate (ASA) content and ascorbate peroxidase (APOX) activity in the tissues of the grain aphid Sitobion avenae and the bird cherry-oat aphid Rhopalosiphum padi were studied. Among the aphid morphs, the highest ASA content and APOX activity were noted for larvae and the lowest for wingless apterae. When exposed to o-dihydroxyphenols, aphids of both species contained significantly lower concentrations of ASA and higher APOX activity than the controls. Among the studied compounds, caffeic acid had the strongest effect on ASA-based antioxidant responses in that caffeic acid caused a 5-fold decrease of ASA in aphid tissues. The influences of the plant o-dihydroxyphenols on antioxidant defense mechanisms within the cereal aphid species are discussed.

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Ascorbate peroxidase: A novel antioxidant enzyme in insects

Cited by 104 publications

References 39 publications

Antioxidant defense response in a galling insect

Antioxidant defense response in a galling insect

Ghrelin effects on midgut tissue antioxidative defense and glutathioneS-transferase activity in Lymantria dispar (Lepidoptera)

Antioxidant defense mechanisms of cereal aphids based on ascorbate and ascorbate peroxidase

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