Ascorbate peroxidase gene family in tomato: its identification and characterization

Najami, Naim; Janda, Tibor; Barriah, Waseim; Kayam, Galya; Tal, Moshe; Guy, Micha; Volokita, Micha

doi:10.1007/s00438-007-0305-2

Cited by 115 publications

(66 citation statements)

References 45 publications

(70 reference statements)

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“…The antioxidative systems also play an important role in protection against ROS under saline conditions (Mittova et al, 2004;Najami et al, 2008). In the present experiments, the activities of certain antioxidant enzymes, namely GR, catalase and APX, were significantly higher in salt-acclimated plants, contributing to the reduced degradation of chlorophyll molecules.…”

Section: Discussionsupporting

confidence: 45%

Salt acclimation processes in wheat

Janda

Darkó

Shehata

et al. 2016

Plant Physiology and Biochemistry

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Young wheat plants (Triticum aestivum L. cv. Mv Béres) were exposed to 0 or 25 mM NaCl for 11 days (salt acclimation). Thereafter the plants were irrigated with 500 mM NaCl for 5 days (salt stress). Irrigating the plants with a low concentration of NaCl successfully led to a reduction in chlorotic symptoms and in the impairment of the photosynthetic processes when the plants were exposed to subsequent high-dose salt treatment. After exposure to a high concentration of NaCl there was no difference in leaf Na content between the salt-acclimated and non-acclimated plants, indicating that salt acclimation did not significantly modify Na transport to the shoots. While the polyamine level was lower in salt-treated plants than in the 2 control, salt acclimation led to increased osmotic potential in the leaves. Similarly, the activities of certain antioxidant enzymes, namely glutathione reductase, catalase and ascorbate peroxidase, were significantly higher in salt-acclimated plants. The results also suggest that while SOS1, SOS2 or NHX2 do not play a decisive role in the salt acclimation processes in young wheat plants; another stress-related gene, WALI6, may contribute to the success of the salt acclimation processes. The present study suggested that the responses of wheat plants to acclimation with low level of salt and to treatment with high doses of salt may be fundamentally different.

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Section: Discussionsupporting

confidence: 45%

Salt acclimation processes in wheat

Janda

Darkó

Shehata

et al. 2016

Plant Physiology and Biochemistry

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“…CAT and APX are the important components of the antioxidant system, played a vital role in eliminating and maintaining the threshold level of H 2 O 2 in a range of cellular organelles (Najami et al 2008). Our results demonstrated that CAT and APX activities significantly induced at higher concentrations of elicitors which signify the production of ROS due to oxidative stress.…”

Section: Discussionmentioning

confidence: 53%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

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Improvement of the host resistance by using hazard free chemical elicitors is emerging as an alternative approach in the field of plant disease management. In our present work, we have screened the efficacy and possible mechanism of abiogenic elicitors like Dipotassium hydrogen orthophosphate (K 2 HPO 4 ), Oxalic acid (OA), Isonicotinic acid (INA), Salicylic acid (SA), Acetylsalicylate (AS), Arachidonic acid (AA) and Calcium chloride (CaCl 2 ) to stimulate innate immune responses in Lycopersicum esculentum Mill. Excised tomato leaves, treated with elicitors at three different concentrations, were found to stimulate defense and antioxidative enzymes, total phenol and flavonoid content after 24 h of incubation. CaCl 2 (0.5 %) followed by INA (2.5 mM) were found most effective in activation of all such defense molecules in tomato leaves. Furthermore, nitric oxide (NO), a key gaseous mediator in plant defense signaling, was also measured after subsequent elicitor application. Higher doses of elicitors showed an elevated level of reactive oxygen species (ROS) generation, enhanced lipid peroxidation rate and proline content, which indicates the extent of abiotic stress generation on the leaves. However, ROS production, lipid peroxidation rate and proline concentration remain significantly reduced as a result of CaCl 2 (0.5 %) and INA (2.5 mM) application. A sharp increase of total chlorophyll content was also recorded due to treatment of CaCl 2 (0.5 %). These results demonstrate the effects of different abiogenic elicitors to regulate the production of defense molecules. Results also suggest that among all such chemicals, CaCl 2 (0.5 %) and INA (2.5 mM) can be used as a potential elicitor in organic farming of tomato.

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“…Recent genome-wide analysis in higher plants indicated that APX belongs to a multigenic family, since there are nine genes in Arabidopsis, eight in rice, and seven in tomato. [43][44][45] These APX families are further classified into three subfamilies according to subcellular localization, viz., chloroplastic, cytosolic, and microbody isoenzymes. 5) Chloroplastic isoenzymes have stroma-soluble (sAPX) and thylakoid membranebound (tAPX) forms.…”

Section: Enzymatic and Molecular Properties Of Apx Isoenzymesmentioning

confidence: 99%

Recent Advances in Ascorbate Biosynthesis and the Physiological Significance of Ascorbate Peroxidase in Photosynthesizing Organisms

Ishikawa

Shigeoka

2008

Bioscience, Biotechnology, and Biochemistry

250

168

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Ascorbate (AsA), the most abundant water-soluble redox compound in plants and eukaryotic algae, has multiple functions. There is compelling genetic evidence that the biosynthesis of AsA proceeds via a D-mannose/ L-galactose pathway and is the most significant source of AsA in plants. AsA plays important roles in antioxidative defense, particularly via the AsA/glutathione cycle. AsA peroxidase (APX) plays a central role in the cycle and is emerging as a key enzyme in cellular H 2 O 2 metabolism. Plants possess diverse APX isoenzymes in cellular compartments, including the chloroplast, cytosol, and microbody. In algae, however, the number and distribution of APX proteins are quite limited. Recent progress in molecular biological analysis of APX isoenzymes has revealed elaborate mechanisms for the tissue-dependent regulation of two chloroplastic APX isoenzymes by alternative splicing, and for redox regulation of cytosolic APX gene expression in response to light stress. Furthermore, transgenic plants overexpressing a chloroplastic APX isoenzyme enable us to evaluate the behavior of the enzyme under conditions of photo-oxidative stress. Molecular physiological analysis has revealed that cytosolic APX is part of the system modulating the cellular H 2 O 2 level in redox signaling.

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Ascorbate peroxidase gene family in tomato: its identification and characterization

Cited by 115 publications

References 45 publications

Salt acclimation processes in wheat

Salt acclimation processes in wheat

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Recent Advances in Ascorbate Biosynthesis and the Physiological Significance of Ascorbate Peroxidase in Photosynthesizing Organisms

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