Ascorbate Restores Lifespan of Superoxide-dismutase Deficient Yeast

Krzepiłko, Anna; Święciło, Agata; Wawryn, Jarosław; Zadrag, Renata; Koziol, Sabina; Bartosz, Grzegorz; Biliński, T

doi:10.1080/10715760410001717327

Cited by 24 publications

(16 citation statements)

References 26 publications

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“…Similar results have already been obtained for hamster cells exposed to L-ascorbic acid. 19 Of interest, Krzepilko et al 20 have recently shown that ascorbate can restore the life-span of sod1 mutants and that this protection is probably due to the scavenging of the radical superoxide. In the same way, sod1∆ was protected by L-ascorbic acid against oxidative damage induced by paraquat, as observed in Figure 2.…”

Section: Discussionmentioning

confidence: 98%

Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains ofSaccharomyces cerevisiae

et al. 2006

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Much has been published on the non-enzymatic antioxidant L-ascorbic acid (vitamin C), but even so its interaction with endogenous cellular defense systems has not yet been fully elucidated. Our study investigated the antioxidant activity of L-ascorbic acid in wild-type strain EG103 (SOD) Saccharomyces cerevisiae and isogenic mutant strains deficient in cytosolic superoxide dismutase (sod1delta), mitochondrial superoxide dismutase (sod2delta) or both (sod1delta sod2delta), metabolizing aerobically or anaerobically with and without the stressing agent paraquat. The results show that during both aerobic and anaerobic metabolism there was a significant increase in the survival of both wild-type S. cerevisiae cells and the mutant cells (sod1delta, sod2delta and sod1delta sod2delta) when pretreated with L-ascorbic acid before exposure to paraquat. Exposure to paraquat resulted in higher catalase activity but this significantly decreased when the cells were pre-treated with L-ascorbic acid. These results demonstrate that due to the damage caused by paraquat, the antioxidant protection of L-ascorbic acid seems to be mediated by catalase levels in yeast cells.

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Section: Discussionmentioning

confidence: 98%

Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains ofSaccharomyces cerevisiae

et al. 2006

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“…The prooxidative effects of some antoxidants revealed in the cellular tests may contribute to the limited effectiveness of the in vivo applications of antioxidants [32 -34] and be a complication of the use of yeast as a test system for antioxidants [1] (Zyracka et al, submitted). On the other hand, the cellular system of testing antioxidants may allow for a preselection of antioxidant compounds which are of potential therapeutic value.…”

Section: Discussionmentioning

confidence: 99%

“…We have found that ascorbate is able to protect Saccharomyces cerevisiae lacking CuZn-superoxide dismutase (SOD1) against toxicity of pure oxygen atmosphere, shortening of replicative life span [1] and auxotrophy for lysine and methionine (Zyracka et al, submitted). Ascorbate restored also growth of Dsod1 mutant on a hypertonic medium, alleviating the effect of hypertonicity mediated by oxidative stress.…”

Section: Introductionmentioning

confidence: 97%

Limited Effectiveness of Antioxidants in the Protection of Yeast Defective in Antioxidant Proteins

Lewińska

Biliński

Bartosz

2004

Free Radical Research

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Efficacy of several antioxidants in the protection of the yeast Saccharomyces cerevisiae mutants deficient in CuZnSOD and deficient in glutaredoxin 5 to growth restriction induced by oxidants was studied. Ascorbate and glutathione protected the Deltasod1 and Deltagrx5 mutants against the effects of t-butyl hydroperoxide and cumene hydroperoxide, Deltasod1 mutants against oxytetracycline and Deltagrx5 mutants against menadione and 2,2'-azobis-(2-amidinopropane). However, Tempol, Trolox and melatonin were much less effective, showing prooxidative effects and, at high concentrations, hampering the growth of the mutants in the absence of exogenous oxidants. These results point to a complication of cellular effects of antioxidants by their prooxidative effects and to the usefulness of cellular tests to evaluate the biological effectiveness of antioxidants.

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“…This effect is mediated by oxidative stress, since the SOD1Δ strain is oversensitive to hypertonic medium compared to wild-type yeast, and ROS accumulate in yeast cells exposed to hypertonic media (Garay-Arroyo et al, 2003;Koziol et al, 2005). These obvious phenotypes of SOD1Δ could be reversed by the addition of L-ascorbic acid (AsA), a potent antioxidant (Krzepilko et al, 2004;Zyracka et al, 2005a;Koziol et al, 2005, Saffi et al, 2006. Compensation of the Sod1 defect by an antioxidant implies that the mutant yeast can be used as a biosensor to antioxidants (Zyracka et al, 2005b).…”

Section: Introductionmentioning

confidence: 99%

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

Tamura¹,

Wada²,

Hase³

et al. 2010

FSTR

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Osmotic stress hampers the growth of Saccharomyces cerevisiae, and this effect is mediated by oxidative stress. A simple test for the biological evaluation of antioxidants was developed on the basis of recovery of growth delay of Cu, Zn-superoxide dismutase-lacking yeast (SOD1Δ) under hyperosmosis. The SOD1Δ strain suffered from growth inhibition in a medium containing 1.7 M sorbitol. Protein oxidation in SOD1Δ cells was greatly increased by exposure to a hypertonic medium, indicating the accumulation of reactive oxygen species followed by oxidation of cellular materials. The hyperosmosis-induced growth arrest of SOD1Δ was abolished by the addition of L-ascorbic acid to the medium; the antioxidant effect depended on the concentration, ranging from 1 to 10 mM. Cysteine, N-acetyl-cysteine, and glutathione were also able to restore the growth of SOD1Δ cells. High concentrations of these thiol compounds proved less effective, probably due to adverse effects of an excess of these antioxidants. No growth restoration was seen for typical polyphenol antioxidants, including curcumin, quercetin and catechin.

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Ascorbate Restores Lifespan of Superoxide-dismutase Deficient Yeast

Cited by 24 publications

References 26 publications

Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains ofSaccharomyces cerevisiae

Antioxidant activity of L-ascorbic acid in wild-type and superoxide dismutase deficient strains ofSaccharomyces cerevisiae

Limited Effectiveness of Antioxidants in the Protection of Yeast Defective in Antioxidant Proteins

A Simple Growth Test of a Saccharomyces cerevisiae Cu, Zn-Superoxide Dismutase-Deficient Mutant in Hypertonic Medium for Biological Evaluation of Antioxidants

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