Ascorbic acid and its pro-oxidant activity as a therapy for tumours of oral cavity – A systematic review

Putchala, Manisha Chandini; Ramani, Pratibha; Sherlin, Herald J; Premkumar, Priya; Natesan, Anuja

doi:10.1016/j.archoralbio.2013.01.016

Cited by 139 publications

(76 citation statements)

References 31 publications

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“…Extensive study has been done on ascorbic acid as an antioxidant and pro-oxidant with anticarcinogenic and anticancer properties [51]. Ascorbic acid has been shown to be cytotoxic to MCF-7 and HT-29 cells [52,53] and to induce cell death through the generation of H 2 O 2 [54].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration

Tang

Jayakumar

Fung

et al. 2013

BMC Complement Altern Med

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BackgroundCoriandrum sativum is a popular culinary and medicinal herb of the Apiaceae family. Health promoting properties of this herb have been reported in pharmacognostical, phytochemical and pharmacological studies. However, studies on C. sativum have always focused on the aerial parts of the herb and scientific investigation on the root is limited. The aim of this research was to investigate the antioxidant and anticancer activities of C. sativum root, leaf and stem, including its effect on cancer cell migration, and its protection against DNA damage, with special focus on the roots.MethodsPowdered roots, leaves and stems of C. sativum were extracted through sequential extraction using hexane, dichloromethane, ethyl acetate, methanol and water. Total phenolic content, FRAP and DPPH radical scavenging activities were measured. Anti-proliferative activitiy on the breast cancer cell line, MCF-7, was assayed using the MTT assay. Activities of the antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, and of the caspases-3, -8 and -9 were assayed on treatment with the extract. Cell cycle progression was analysed using flow cytometry. The scratch motility assay was used to assess inhibition of MCF-7 cell migration. DNA damage in 3 T3-L1 fibroblasts was evaluated by the comet assay. The components in the extract were identified by HPLC and GC-MS.ResultsThe ethyl acetate extract of C. sativum roots showed the highest antiproliferative activity on MCF-7 cells (IC50 = 200.0 ± 2.6 μg/mL) and had the highest phenolic content, FRAP and DPPH scavenging activities among the extracts. C. sativum root inhibited DNA damage and prevented MCF-7 cell migration induced by H2O2, suggesting its potential in cancer prevention and inhibition of metastasis. The extract exhibited anticancer activity in MCF-7 cells by affecting antioxidant enzymes possibly leading to H2O2 accumulation, cell cycle arrest at the G2/M phase and apoptotic cell death by the death receptor and mitochondrial apoptotic pathways.ConclusionsThis study is the first report on the antioxidant and anticancer properties of C. sativum root. The herb shows potential in preventing oxidative stress-related diseases and would be useful as supplements used in combination with conventional drugs to enhance the treatment of diseases such as cancer.

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Section: Discussionmentioning

confidence: 99%

Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration

Tang

Jayakumar

Fung

et al. 2013

BMC Complement Altern Med

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“…22 Studies have reported that the pro-oxidant mechanisms of ascorbic acid include an ability to reduce metal ions such as Fe 3+ and Cu 3+ , a process that generates free radicals such as hydroxyl radical, which interact with DNA, causing breaks in the phosphodiester bonds in addition modifications in the bases, generating induced cytotoxicity. 23 Some research indicate another antitumor mechanism of action, the proliferation of natural killers (NK) cells without affecting its normal functions. According to them, these cells have the ability to "kill" tumor cells without the need for sensitization of direction, and that ascorbic acid promotes their proliferation.…”

Section: Resultsmentioning

confidence: 99%

Ascorbic acid in the prevention and treatment of cancer

Mata

Carvalho

Alencar

et al. 2016

Rev. Assoc. Med. Bras.

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Mata aMOF da et al. 680rev assoC med bras 2016; 62(7):680-686 This review is aimed at the systematic mapping of ascorbic acid in the prevention and/or treatment of cancer in clinical and non-clinical studies from 2011 to 2015, in order to understand dose-response variations as well as its mechanisms of action as an antioxidant and antitumor agent. Seventy-eight articles were retrieved from the PubMed/Bireme database, of which only 30 included ascorbic acid in the prevention and/or treatment of cancer. However, there are controversies regarding doses and a lack of clinical studies featuring its mechanism of action more clearly. Other studies are needed to understand dose-response variations, as well as its targeting mechanisms of action, both as an antioxidant and antitumor agent, to assist treatment and prevention of cancer, aiming at better quality of life for both patients and the general population.

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“…The ascorbic acid (Sigma, St. Louis, MO) was solubilized in 50 mM phosphate buffer (pH 7.4) till the final concentration of 2 µg/mL. This concentration was predetermined because it may not produce pro-oxidant effects, as reported by Putchala [25] and Bartel [26].…”

Section: Chemicalsmentioning

confidence: 99%

Ascorbic acid modulates doxorubicin and cyclophosphamide-induced cytogenetic damages in Sarcoma 180 cells

Alencar¹,

Islam²,

Rocha³

et al. 2016

Int Arch Med

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Cancer is a public health global problem. Cyclophosphamide (CPA) and Doxorubicin (DOX) are used in chemotherapy, especially by generating reactive oxygen species. The clinical use of ascorbic acid during chemotherapy also raises several controversies due to its antagonistic effects on antineoplastic agent. In this sense, this study aims to evaluate the effects of ascorbic acid (2 µg/mL) on the modulation of cytogenetic damage induced by CPA (20 µg/mL) and DOX (2 µg/ mL), as well as their interaction (AC protocol) on Sarcoma 180 tumor cells. Cytogenetic damage classified as apoptosis, necrosis, micronuclei, buds and nucleoplasmic bridges were linked to the CytokinesisBlock Micronucleus Assay application. CPA and DOX induce significant (p<0.05) increases in apoptosis, necrosis and micronuclei in the cell types tested. The damage on Sarcoma 180 cells was modulated by ascorbic acid with percentage modulation in more than 70% of CPA relative to apoptosis and micronuclei, and 32% to necrosis. The damage of DOX has been modulated by 70% to apoptosis, 40% to necrosis, rather than micronuclei, whereas, in AC protocol, modulations were observed by 52% to apoptosis and 32% and 40% to necrosis and micronuclei, respectively. There was no significance in relation to the nuclear buds and nucleoplasmic bridges. In addition, ascorbic acid did not show any effect. These results are other studies, which indicate hazards to chemotherapy effectiveness due to the antioxidant effects

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Ascorbic acid and its pro-oxidant activity as a therapy for tumours of oral cavity – A systematic review

Cited by 139 publications

References 31 publications

Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration

Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration

Ascorbic acid in the prevention and treatment of cancer

Ascorbic acid modulates doxorubicin and cyclophosphamide-induced cytogenetic damages in Sarcoma 180 cells

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