Ascorbic Acid and Polyphenols Mediated Green Synthesis of Silver Nanoparticles from Tagetes erecta L. Aqueous Leaf Extract and Studied Their Antioxidant Properties

Abstract

Silver nanoparticle synthesis of the leaf extract Tagetes erecta L. enriched with ascorbic acid and polyphenols has been investigated. The color of the golden yellow extract has changed to pinkish-brown due to the reduction of Ag+ to the colloidal solution of AgNPs and a sharp absorption peak at 420 nm under the UV-Vis spectrophotometer. In addition, the Fourier Transfer Infrared Spectroscopy (FTIR) estimation was completed in order to recognize and identify the biomolecules present in the extract acting as a reducing and capping agent for the AgNPs. The X-ray diffraction (XRD) peaks at (111), (201), (220), and (311) confirm the presence of monoclinic crystals in the solution. The morphology and size of the particles were provided by transmission electron microscopy (TEM) images of AgNPs. At a scale of 100 nm, synthesized AgNPs were predominantly spherical with a size range of 7-35 nm. In comparison to 7.39 mg/100 g in AgNPs, aqueous leaf extract was 55.14 mg/100 g higher in ascorbic acid. The phenolic and flavonoid content of extract was 52.54 ± 2.15 mg (GAE/100 g) and 15.43 ± 0.34 mg (QE/mL), and the colloidal AgNP solution was 21.45 ± 1.15 mg (GAE/100 g) and 8.05 ± 2.42 mg (QE/mL), respectively. Phenolic and flavonoid contents play a major role as a reducing agent and reduce the precursor AgNO3 into AgNPs. The DPPH scavenging assay also assessed the antioxidant properties of extract and its derived AgNPs. As compared antioxidant value to aqueous leaf extract (mg/mL), higher percentage inhibition (PI) was found in AgNPs and free-radical scavenging activity of extract and AgNPs were directly linked to their concentrations. Results of this research have discovered a higher potential for free-radical scavenging AgNPs and will help to develop new and more potent antioxidants for the treatment of different diseases caused by oxidative stress; the higher antioxidant properties bearing AgNPs might be used.

Green Synthesis of Silver Nanoparticles from Root Extracts of Rubus ellipticus Sm. and Comparison of Antioxidant and Antibacterial Activity

“…Change of color from golden yellow to pinkish brown was observed in the reduction of Ag + to Ag °in the colloidal solution. A sharp absorption peak at 420 nm was observed in the UV-visible spectroscopy during the biosynthesis of AgNPs from the Tagetes erecta aqueous leaf extracts [33]. The strong broad absorption band obtained between 405 and 430 nm for silver nanoparticles indicates that the spherical particles were dispersed without aggregation [34,35].…”

Eco-Friendly Synthesis and Comparative In Vitro Biological Evaluation of Silver Nanoparticles Using Tagetes erecta Flower Extracts

“…To the best of our knowledge, this is the first report on the evaluation of the antioxidant activity of AgNPs obtained using T. erecta flower extracts through the two chosen methods. One other previous study conducted on AgNPs obtained using T. erecta aqueous leaf extract assessed the antioxidant activity through DPPH scavenging assay, showing improved activity for the obtained nanoparticles compared to the initial extract [37].…”

“…For example, Dhuldhaj et al described AgNPs obtained using an aqueous extract of T. erecta leaves as spherical and polydisperse, with an average diameter of 30 nm [24]. Similarly, for an aqueous extract of T. erecta leaves, Tyagi et al obtained mostly spherical AgNPs with an average size of 22 nm [37].…”

Green Synthesis of Piper nigrum Copper-based Nanoparticles: In-Silico Study and ADMET Analysis to Assess Their Antioxidant, Antibacterial, and Cytotoxic Effects

“…The ltrate solution was then stored at 4℃for further analysis. [39] Synthesis of P. nigrum fruit extract copper nanoparticles (PN-CuNPs) PN-CuNPs were synthesized by adding 10 ml of fruit extract drop wise to the 200 ml of copper acetate solution and stirring with magnetic stirrer. The colour change of the solution from brown to blue represents the formation of PN-CuNPs.…”

“…Most of the alkamides tested revealed moderate intestinal absorption characteristics as evidenced by the ADMET plot in Fig.12where they were in the 99% absorption ellipse with only compounds 52-54 located outside the 99% absorption range. The BBB penetration with values 0 and 1 was either very high or high penetration except for compounds[35][36][37][38][39] lying outside the 99% con dence ellipse of BBB absorption (Table4. and Fig.12.).…”