Ascorbic acid and retinol palmitate modulatory effect on omeprazole-induced oxidative damage, and the cytogenetic changes in S. cerevisiae and S180 cells

Paz, Márcia Fernanda Correia Jardim; Braga, Antonio Lima; Meneses, Ag-Anne Pereira Melo de; Carvalho, Rezanio Martins; Aguiar, Rai Pablo Sousa de; Cronemberger, Lara Arrais Chaves; Silva, Milena Braga Soares da; Lima, Róbson Borges de; Alencar, Marcus Viní­cius Oliveira Barros de; Duarte, Jozelda Lemos; Islam, Muhammad Torequl; Sousa, João Marcelo de Castro e; Mubarak, Mohammad S.; Melo‐Cavalcante, Ana Amélia de Carvalho

doi:10.1016/j.cbi.2019.108776

Cited by 7 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, ascorbic acid easily reacts with CP to form a low-toxic salt [33]. Available data indicates that the polyphenolic compounds in chokeberries may also have significant cytoprotective potential, which has been described in a number of works preceding our study [34].…”

Section: Discussionsupporting

confidence: 55%

Radical Scavenging Actions and Immunomodulatory Activity of Aronia melanocarpa Propylene Glycol Extracts

Bushmeleva

Vyshtakalyuk

Terenzhev

et al. 2021

Plants

View full text Add to dashboard Cite

Researchers are attracted to the wide-ranging, useful components in Aronia melanocarpa berries. They are searching for the most effective ways to extract the active substances that can enhance the body’s protective properties. The current study presents detailed information about the extracts from A. melanocarpa fruits frozen and dried under mild conditions and their chemical composition. In Wistar rats with induced immunosuppression, the effect of chokeberry fruit extracts on the leukocyte formula, phagocytic activity, and cytokine system was studied. It was shown that the A. melanocarpa frozen fruit extract contains more anthocyanins, sugars, and ascorbic acid, and has a more pronounced antioxidant activity determined by the ability to bind APPH-radicals. Moreover, the extract showed membrane-protective and cytoprotective properties against RPMI-1788 cell line. The extract from dried raw material shows a higher antioxidant activity due to the ability to bind DPPH-radicals. It was revealed that extracts from A. melanocarpa fruits promote rapid immune system recovery in rats, normalize the leukocyte count, and improve monocyte and neutrophil phagocytic indicators. Research on the cytokine profile revealed that the anti-inflammatory properties in A. melanocarpa extracts were more pronounced in dried extracts. For several cytokines, a normalization of quantity was noted.

show abstract

Section: Discussionsupporting

confidence: 55%

Radical Scavenging Actions and Immunomodulatory Activity of Aronia melanocarpa Propylene Glycol Extracts

Bushmeleva

Vyshtakalyuk

Terenzhev

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…To confirm this, antioxidant's enzymatic activities, erythrocyte's size, PS exposure at cell surface, hemolytic activity and confirmation of calcium role in the induction of suicidal death of erythrocytes were determined. The omeprazole concentrations used in the study were lower than the concentrations already reported to treat S. cerevisiae and S180 cells (18). Figure 1 is illustrating that 48 hour exposure of erythrocytes to omeprazole (0.5, 1 & 1.5 mM) resulted in significant decrease in activities of superoxide dismutase at 1.5 mM with respect to control.…”

Section: Resultsmentioning

confidence: 85%

“…Isolated erythrocytes were stored in separate micro-centrifuge tubes. In vitro incubation of erythrocytes was performed at a hematocrit of 0.4% in ringer solution (pH 7.4) that contain (in mM) MgSO 4 1, NaCl 125, KCl 5, glucose 5, CaCl 2 1, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES) 32 at 37 o C for 48 h. 17 Isolated erythrocytes were than exposed to lower concentrations (0.5, 1 and 1.5 µM) of omeprazole (Sigma-Aldrich, USA) as indicated by Paz et al., 2019 18 while the control cells were completely untreated. Dimethyl sulfoxide (DMSO) was used as solvent for omeprazole.…”

Section: Methodsmentioning

confidence: 99%

Induction of Erythrocyte Shrinkage by Omeprazole

et al. 2020

View full text Add to dashboard Cite

Omeprazole, a proton pump inhibitor blocks the H+/K+-ATPase channels of gastric parietal cells. It is used for the treatment of peptic ulcer. Prolonged use of omeprazole may involve in inducing anemia. The key marker of eryptosis includes membrane blebbing, cell shrinkage and phosphatidylserine (PS) exposure at the cell surface. In current study, the eryptotic, oxidative as well as hemolytic effects of therapeutical doses (0.5, 1 and 1.5 µM) of omeprazole were investigated after exposing erythrocytes for 48 hours. Investigation of eryptosis was done by cell size measurement, PS exposure determination and calcium channel inhibition. As a possible mechanism of omeprazole induced eryptosis, oxidative stress was investigated by determining the catalase, glutathione peroxidase and superoxide dismutase activities. Similarly, necrotic effect of omeprazole on erythrocytes was also evaluated through hemolysis measurement. Results of our study illustrated that 1.5 µM of omeprazole may induce significant decrease in superoxide dismutase, glutathione peroxidase and catalase activities as well as triggered the erythrocytes shrinkage, PS exposure and hemolysis. Role of calcium was also confirmed in inducing erythrocyte shrinkage. It is concluded that the exposure of erythrocytes with 1.5 µM omeprazole may enhance the rate of eryptosis and hemolysis by inducing oxidative stress.

show abstract

“…In non-clinical studies, toxicogenic effects of OME were identi ed in Allium cepa (Braga et al, 2018), as well as oxidative effects in Saccharomyces cerevisiae and cytogenetics in Sarcoma 180 cells (Paz et al, 2019). Thus, in continuity with the previousinvestigations, the present study aimed to evaluate the possible cytogenetic damages of the use and/or therapy of OME in the gastric epithelium of patients with gastritis, with and without H. pylori infection, by the application of the micronucleus test and dosages of catalase and superoxide dismutase.…”

Section: Introductionmentioning

confidence: 99%

“…Oxidative damage can be one of the OME mechanisms for inducing changes in genetic material in gastric epithelium cells, as it can produce H 2 O 2 when it binds to protein C283, which contains CACT, and C136 generating beta oxidation of fatty acids(Tonazzi et al, 2013). OME can induce oxidative damage in S. cerevisiae, in addition to cytogenetic damage in Sarcoma 180 cells(Paz et al, 2019).…”

mentioning

confidence: 99%

Evaluation of Mutagenesis, Necrosis and Apoptosis induced by Omeprazole in Stomach Cells of Patients with Gastritis

Mata

Paz²,

Menezes³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Gastritis is a superficial and prevalent inflammatory lesion that is considered a public health concernoncecause gastric ulcers and gastric cancer, especially due to Helicobacter pylori infection. Proton pump inhibitors, such as omeprazole, are the most widely used drugs in order to treat this illness. The aim of the study was evaluating the cytogenetic effects of omeprazole in stomach epithelial cells of patients with gastritis in presence and absence of H. pylori, using to this the application of cytogenetic biomarkers and measurements of catalse and superoxide dismutase. Methods: The study included 152 patients from the Gastroenterology Outpatient Clinic of Hospital Getúlio Vargas, Teresina - PI, that reported the continuous and prolonged use of omeprazole in doses of 20, 30 and 40 mg/kg. The participants were divided into groups: (1) patients without gastritis (n = 32); (2) patients without gastritis but with use of OME (n = 24); (3) patients with gastritis (n = 26); (4) patients with gastritis undergoing OME therapy (n = 26); (5) patients with gastritis and H. pylori (n = 22) and (6) patients with gastritis and H. pylori on OME therapy (n = 22). Results: OME induces cytogenetic risks in the epithelium of the stomach due to the formation of micronuclei (group 6> 1,2,3,4,5; group 5> 1,2,3; group 4> 1,2,3); bridges (groups 4 and 6> 1,2,3,5 and group 2> 3,5); buds (groups 2,4,6>, 1,3,5); binucleated cells (group 6> 1,2,3,4,5; group 4> 1,2,3); groups 2 and 3> 1); picnoses (group 6> 1,2,3,4,5), groups 2 and 5> 1,3; group 4> 1,2,3,5); cariorrexis (groups 6 and 4> 1,2,3,5; groups 2,3,5> 1) and karyolysis (groups 2,4, and 6> 1,3,5; groups 3 and 5> 1). These data show that omeprazole induces cytogenetic risks, especially due to infection with H. pylori, thus indicating the clastogenic and/or aneugenic effects, chromosomes changing, gene expression, cytotoxicity and apoptosis. Conclusions: These risks can be attributed to several mechanisms that are still unclear, including oxidative damage, as observed by increases in catalase and superoxide dismutase. Positive correlations between these antioxidant enzymes were obtained with the formation of micronuclei, and negative for picnoses. Thus, the continuous and prolonged use of omeprazole induces genetic instability, which can be monitored, in cytogenetic analyzes, as anticipation for cancer, especially gastric.

show abstract

Ascorbic acid and retinol palmitate modulatory effect on omeprazole-induced oxidative damage, and the cytogenetic changes in S. cerevisiae and S180 cells

Cited by 7 publications

References 21 publications

Radical Scavenging Actions and Immunomodulatory Activity of Aronia melanocarpa Propylene Glycol Extracts

Radical Scavenging Actions and Immunomodulatory Activity of Aronia melanocarpa Propylene Glycol Extracts

Induction of Erythrocyte Shrinkage by Omeprazole

Evaluation of Mutagenesis, Necrosis and Apoptosis induced by Omeprazole in Stomach Cells of Patients with Gastritis

Contact Info

Product

Resources

About

Ascorbic acid and retinol palmitate modulatory effect on omeprazole-induced oxidative damage, and the cytogenetic changes in S. cerevisiae and S180 cells

Cited by 7 publications

References 21 publications

Radical Scavenging Actions and Immunomodulatory Activity of Aronia melanocarpa Propylene Glycol Extracts

Radical Scavenging Actions and Immunomodulatory Activity of Aronia melanocarpa Propylene Glycol Extracts

Induction of Erythrocyte Shrinkage by Omeprazole

Evaluation of Mutagenesis, Necrosis and Apoptosis induced by Omeprazole in Stomach Cells of Patients with Gastritis

Contact Info

Product

Resources

About

Ascorbic acid and retinol palmitate modulatory effect on omeprazole-induced oxidative damage, and the cytogenetic changes in S. cerevisiae and S180 cells