Ascorbic acid enhances the expression of type 1 and type 4 collagen and SVCT2 in cultured human skin fibroblasts

Kishimoto, Yuki; Saito, Norikatsu; Kurita, Katsumi; Shimokado, Kentaro; Maruyama, Naoki; Ishigami, Akihito

doi:10.1016/j.bbrc.2012.11.110

Cited by 89 publications

(82 citation statements)

References 26 publications

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“…Further, it has also been reported that AA 2-phosphate, a long-acting vitamin C derivative, stimulates both cell growth and the expression of mRNA for type III collagen in human osteoblast-like MG-63 cells and in normal human osteoblasts, as well as in human bone marrow mesenchymal stem cells, but not the expression of type I collagen in these cells [22]. However, in another study Kishimoto et al [23] have observed that AA induced the expression of type 1 and type 4 collagen and SVCT2 in cultured human skin fibroblasts. Collagen constitutes the principal protein of skin, bones, teeth, cartilage, tendons, blood vessels, heart valves, inter vertebral discs, cornea, eye lens.…”

Section: Biochemical Functions Of Vitamin Cmentioning

confidence: 95%

Vitamin C in Disease Prevention and Cure: An Overview

et al. 2013

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The recognition of vitamin C is associated with a history of an unrelenting search for the cause of the ancient haemorrhagic disease scurvy. Isolated in 1928, vitamin C is essential for the development and maintenance of connective tissues. It plays an important role in bone formation, wound healing and the maintenance of healthy gums. Vitamin C plays an important role in a number of metabolic functions including the activation of the B vitamin, folic acid, the conversion of cholesterol to bile acids and the conversion of the amino acid, tryptophan, to the neurotransmitter, serotonin. It is an antioxidant that protects body from free radical damage. It is used as therapeutic agent in many diseases and disorders. Vitamin C protects the immune system, reduces the severity of allergic reactions and helps to fight off infections. However the significance and beneficial effect of vitamin C in respect to human disease such as cancer, atherosclerosis, diabetes, neurodegenerative disease and metal toxicity however remains equivocal. Thus further continuous uninterrupted efforts may open new vistas to understand its significance in disease management.

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Section: Biochemical Functions Of Vitamin Cmentioning

confidence: 95%

Vitamin C in Disease Prevention and Cure: An Overview

et al. 2013

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“…Although most of animals can synthesize vitamin C but it cannot be synthesized by humans being and they are totally dependent upon dietary intake to meet their demands. Vitamin C is known to be essential for enhancing immune response (Holmannová et al, 2012), the structural integrity of the intracellular matrix (Nishikimi et al, 1994), formation of collagens (Jagetia et al, 2007) and hormone synthesis (Kishimoto et al 2013). In addition, numerous studies have reported that vitamin C can function as a tumor prevention agent and human cancer tissues contain elevated levels of ascorbic acid (Padayatty et al, 2006), and this value was approximately 4-fold higher than those found in tissues of normal origin.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C Increases the Level of Lactate Dehydrogenase in Rats ‘Oral Squamous Cell Carcinoma

Hadad¹,

Bioinformatics²

2018

JEBAS

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Since the discovery of vitamin C many studies have illustrated its characteristic effect as a tumor prevention agent. Conversely: the extracellular presence of lactate dehydrogenase (LDH) is associated with cell necrosis and tissue breakdown and is therefore considered as a tumor marker. In present study effects of vitamin C on the levels of oral LDH was estimated during the development of squamous oral carcinoma. Histological findings of study revealed changes in the oral tissue of the animals treated with dimethyl benzanthracene (DMBA) alone or DMBA associated with vitamin C such as moderate dysplasia associated with basal cell hyperplasia. Notably: differentiated oral squamous cell carcinoma was observed in the rats belonging to group injected with DMBA after 6 weeks. Otherwise: anaplastic oral carcinoma was observed after 5 weeks in group treated with DMBA and vitamin C. High levels of LDH were detected throughout the induction of the squamous cell carcinoma phases in comparison to the anaplastic phase. These findings demonstrate that the injection of DMBA in association with vitamin C accelerates the induction of anaplastic carcinoma and that these changes are associated with increased LDH levels in the differentiated squamous carcinoma compared with the undifferentiated carcinomas.

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“…BMSCs are progenitor cells which differentiate into osteoblasts, osteocytes, adipocytes, and cartilage (Prockop et al 1997; Pittenger et al 1999). Vitamin C plays an important role in differentiation of BMSCs into osteoblasts and osteocytes, and in collagen synthesis, cell proliferation and multilayering of osteoblastic cells (Bellows et al 1986; Otsuka et al 2000; Koyama et al 2000; Wang et al 2006; Choi et al 2008; Weiser et al 2009; Li et al 2011; Kishimoto et al 2013). …”

Section: Introductionmentioning

confidence: 99%

The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis

et al. 2015

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Vitamin C is an antioxidant that plays a vital role in various biological processes including bone formation. Previously, we reported that vitamin C is transported into bone marrow stromal cells (BMSCs) through the sodium dependent Vitamin C Transporter 2 (SVCT2) and this transporter plays an important role in osteogenic differentiation. Furthermore, this transporter is regulated by oxidative stress. To date, however, the exact role of vitamin C and its transporter (SVCT2) in ROS regulated autophagy and apoptosis in BMSCs is poorly understood. In the present study, we observed that oxidative stress decreased survival of BMSCs in a dose-dependent manner and induced growth arrest in the G1 phase of the cell cycle. These effects were accompanied by the induction of autophagy, confirmed by P62 and LC3B protein level and punctate GFP–LC3B distribution. The supplementation of vitamin C significantly rescued the BMSCs from oxidative stress by regulating autophagy. Knockdown of the SVCT2 transporter in BMSCs synergistically decreased cell survival even under low oxidative stress conditions. Also, supplementing vitamin C failed to rescue cells from stress. Our results reveal that the SVCT2 transporter plays a vital role in the mechanism of BMSC survival under stress conditions. Altogether, this study has given new insight into the role of the SVCT2 transporter in oxidative stress related autophagy and apoptosis in BMSCs.

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Ascorbic acid enhances the expression of type 1 and type 4 collagen and SVCT2 in cultured human skin fibroblasts

Cited by 89 publications

References 26 publications

Vitamin C in Disease Prevention and Cure: An Overview

Vitamin C in Disease Prevention and Cure: An Overview

Vitamin C Increases the Level of Lactate Dehydrogenase in Rats ‘Oral Squamous Cell Carcinoma

The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis

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