Ascorbic acid‐induced progression of quiescent centre cells from G<sub>1</sub> to S phase

Liso, Rosalia; Innocenti, A. M.; Bitonti, Maria Beatrice; Arrigoni, O.

doi:10.1111/j.1469-8137.1988.tb00284.x

Cited by 120 publications

(68 citation statements)

References 23 publications

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“…The mechanism(s) by which AA and/or GSH influence the development of root meristems is not certain, though attention has focused on the possible roles of these compounds as redox intermediates. The absence of detectable AA in the QC led to experiments in which roots were treated with AA, which stimulated QC cells to divide, and resulted in the suggestion that the QC arises, and/or is maintained, because of a localized depletion of AA (Kerk and Feldman, 1995;Liso et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment

2003

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Embedded within the meristem of all Angiosperm roots is a population of slowly dividing cells designated the quiescent center (QC). In maize roots the QC can constitute upwards of 800-1200 cells, most of which spend an extended period of time (180-200 hours) in the G 1 phase of the cell cycle. How the QC forms and is maintained is not known. Here we report that cells of the QC are characterized by their highly oxidized status. Glutathione and ascorbic acid occur predominately in the oxidized forms in the QC. This is contrasted with the status of these redox intermediates in adjacent, rapidly dividing cells in the root meristem, in which the reduced forms of these two species are favored. Using a redox sensitive fluorescent dye we were able to visualize an overall oxidizing environment in the QC, and we also made comparisons with the adjacent, rapidly dividing cells in the root meristem. Altering the distribution of auxin and the location of the auxin maximum in the root tip activates the QC, and cells leave G1 and enter mitosis. Commencement of relatively more rapid cell division in the QC is preceded by changes in the overall redox status of the QC, which becomes less oxidizing. We discuss how the position of the auxin maximum may influence the redox status of the QC and thereby modulate the cell cycle.

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Section: Introductionmentioning

confidence: 99%

Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment

2003

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“…We showed that this resulted in an increased level of ascorbic acid oxidase (AAO) mRNA, protein, and enzyme activity, as well as the localized depletion of ascorbic acid (AA) within the quiescent center (QC), a group of mitotically inactive cells within the root meristem. Since AA is necessary for the G1 to S transition in the cell cycle in root tips (Liso et al, 1988), and regulation of AA is thought to be dependent on AAO, we proposed that its depletion in root tips may be responsible for the formation and maintenance of the QC (Kerk and Feldman, 1995).…”

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confidence: 99%

Auxin Metabolism in the Root Apical Meristem

Kerk¹,

2000

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Within the root meristem of flowering plants is a group of mitotically inactive cells designated the quiescent center (QC). Recent work links the quiescent state to high levels of the growth regulator auxin that accumulates in the QC via polar transport. This in turn results in elevated levels of the enzyme ascorbic acid oxidase (AAO), resulting in a reduction of ascorbic acid (AA) within the QC and mitotic quiescence. We present evidence for additional interactions between auxin, AAO, and AA, and report that, in vitro, AAO oxidatively decarboxylates auxin, suggesting a mechanism for regulating auxin levels within the QC. We also report that oxidative decarboxylation occurs at the root tip and that an intact root cap must be present for this metabolic event to occur. Finally, we consider how interaction between auxin and AAO may influence root development by regulating the formation of the QC.

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“…Liso et al (1988) and Innocenti et al (1990) showed that tbe root meristetns of Allium cepa L. were stimulated by growing in ascorbic acid solution and tbat tbe quiescent centre was reduced from 1000 to 100 cells within 28 h by many of its cells entering S-phase from Gl. When transferred back to water the number of noncycling cells increased in the quiescent centre region.…”

Section: Methodsmentioning

confidence: 99%

Regeneration of the discrete root epidermis of Pistia stratiotes L. after perturbation of the meristem

Clowes

1992

New Phytologist

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SUMMARYPerturbation of meristems of adventitious roots of Pistia resulted in abnormal pattems of cell division in the quiescent centre including the pole of the normally discrete epidermis. Two of the methods used, radiation from incorporated tritiated thymidine and cold treatment, also produced abnorma) patterns in the lateral primordia present within the apices of the adventitious roots. Cells of the monostromatic epidermal shell divided periclinically in some roots and some primordia where all previous divisions would have been anticlinai. In other meristems, the normaJJy quiescent cells of the stelar and cortical poles grew and divided to displace the former epidermal pole and cap initials.Recovery from the perturbation resulted in the organization of a meristem which assembled a new monostromatic epidermal layer apparently discrete from the new cap initials below and the new cortical pole cells above. The new epidermal pole formed continuously with the old and, though the pattern of planes and rates of mitosis initiating and maintaining it must be determined as in normal ontogeny, some additional influence of the epidermis left intact above the region of abnormal activity appears to operate.

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Ascorbic acid‐induced progression of quiescent centre cells from G₁ to S phase

Cited by 120 publications

References 23 publications

Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment

Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment

Auxin Metabolism in the Root Apical Meristem

Regeneration of the discrete root epidermis of Pistia stratiotes L. after perturbation of the meristem

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Ascorbic acid‐induced progression of quiescent centre cells from G1 to S phase

Cited by 120 publications

References 23 publications

Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment

Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment

Auxin Metabolism in the Root Apical Meristem

Regeneration of the discrete root epidermis of Pistia stratiotes L. after perturbation of the meristem

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Ascorbic acid‐induced progression of quiescent centre cells from G₁ to S phase