Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development

Liang, Dong; Zhu, Tingting; Ni, Zhiyou; Lin, Lijin; Tang, Yi; Wang, Zhihui; Wang, Xun; Wang, Jin; Lv, Xiulan

doi:10.1371/journal.pone.0172818

Cited by 24 publications

(19 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…88 In addition, Liang et al showed that the pattern of ASA metabolism in kiwifruit was consistent with the changing pattern of mRNA expression levels of AO2, APX3, GR1, and DHAR1. 112 Different treatments improved the antioxidant capacity of fruits and vegetables, and there was a certain correlation between antioxidant enzymes and transcription abundance. The activity and transcription levels of SOD, CAT, APX, and GR, as well as the expression levels of AO, APX, DHAR, GR, and GST, increased, leading to a decrease in ROS accumulation.…”

Section: Effect Of Ozone Treatment On Antioxidant-related Proteins Ofmentioning

confidence: 97%

Effects of ozone treatment on the antioxidant capacity of postharvest strawberry

Zhang

et al. 2020

RSC Adv.

View full text Add to dashboard Cite

show abstract

Section: Effect Of Ozone Treatment On Antioxidant-related Proteins Ofmentioning

confidence: 97%

Effects of ozone treatment on the antioxidant capacity of postharvest strawberry

Zhang

et al. 2020

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…The maximum AsA content in sweet cherry (Prunus avium cultivar Hongdeng) fruits is observed during their setting followed by a gradual decrease during fruit development and a moderate increase in the mature fruit (Liang et al, 2017). Nonetheless, AsA content continues to increase with the weight of fresh fruits.…”

Section: Specific Features Of Asa Biosynthesis and Accumulation In Frmentioning

confidence: 99%

“…Full-sized cDNAs of 10 genes involved in the L-galactose pathway of AsA biosynthesis have been described as well as of 10 genes involved in AsA recycling. The expression levels of the genes encoding GDP-L-galactose phosphorylase (GGP2), L-galactono-1,4-lactone dehydrogenase (GalLDH), ascorbate peroxidase (APX3), ascorbate oxidase (AO2), glutathione reductase (GR1), and dehydroascorbate reductase (DHAR1) correlate with the quantitative content of vitamin C during fruit development, which suggests that the joint work of all these genes involved in AsA biosynthesis, degradation, and recycling together regulates the AsA accumulation in sweet cherry fruits (Liang et al, 2017).…”

Section: Specific Features Of Asa Biosynthesis and Accumulation In Frmentioning

confidence: 99%

Vitamin C in fleshy fruits: biosynthesis, recycling, genes, and enzymes

2019

View full text Add to dashboard Cite

L-ascorbic acid (vitamin C) is a plant secondary metabolite that has a variety of functions both in plant tissues and in the human body. Plants are the main source of vitamin C in human nutrition, especially citrus, rose hip, tomato, strawberry, pepper, papaya, kiwi, and currant fruits. However, in spite of the biological significance of L-ascorbic acid, the pathways of its biosynthesis in plants were fully understood only in 2007 by the example of a model plant Arabidopsis thaliana. In the present review, the main biosynthetic pathways of vitamin C are described: the L-galactose pathway, L-gulose pathway, galacturonic and myo-inositol pathway. To date, the best studied is the L-galactose pathway (Smyrnoff–Wheeler pathway). Only for this pathway all the enzymes and the entire cascade of reactions have been described. For other pathways, only hypothetical metabolites are proposed and not all the catalyzing enzymes have been identified. The key genes participating in ascorbic acid biosynthesis and accumulation in fleshy fruits are highlighted. Among them are L-galactose pathway proteins (GDP-mannose phosphorylase (GMP, VTC1), GDP-D-mannose epimerase (GME), GDP-L-galactose phosphorylase (GGP, VTC2/VTC5), L-galactose-1-phosphate phosphatase (GPP/VTC4), L-galactose-1-dehydrogenase (GalDH), and L-galactono1,4-lactone dehydrogenase (GalLDH)); D-galacturonic pathway enzymes (NADPH-dependent D-galacturonate reductase (GalUR)); and proteins, controlling the recycling of ascorbic acid (dehydroascorbate reductase (DHAR1) and monodehydroascorbate reductase (MDHAR)). Until now, there is no clear and unequivocal evidence for the existence of one predominant pathway of vitamin C biosynthesis in fleshy fruits. For example, the L-galactose pathway is predominant in peach and kiwi fruits, whereas the D-galacturonic pathway seems to be the most essential in grape and strawberry fruits. However, in some plants, such as citrus and tomato fruits, there is a switch between different pathways during ripening. It is noted that the final ascorbic acid content in fruits depends not only on biosynthesis but also on the rate of its oxidation and recirculation.

show abstract

“…As a critical metabolite in plants, AsA has several essential functions in plant physiology, participates in the detoxification of ROS, and has an important role in promoting resistance to senescence and numerous environmental stresses, such as high temperature, dehydration stress, high light, ozone, UV-B radiation, and salt stress. Also, AsA operates as a cofactor, taking part in the regulation of some fundamental cellular processes (e.g., photoprotection, the cell cycle, and cell expansion) and biosynthesis of important plant hormones (e.g., including abscisic acid, jasmonic acid, ethylene, and gibberellic acid) ( Smirnoff, 2011 ; Liang et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a L-Gulono-1,4-Lactone Oxidase Like Protein in the Floral Nectar of Mucuna sempervirens, Fabaceae

Zhou

Milne

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

Floral nectar plays important roles in the interaction between animal-pollinated plants and pollinators. Its components include water, sugars, amino acids, vitamins, and proteins. Growing empirical evidence shows that most of the proteins secreted in nectar (nectarines) are enzymes that can tailor nectar chemistry for their animal mutualists or reduce the growth of microorganisms in nectar. However, to date, the function of many nectarines remains unknown, and very few plant species have had their nectar proteome thoroughly investigated. Mucuna sempervirens (Fabaceae) is a perennial woody vine native to China. Nectarines from this species were separated using two-dimensional gel electrophoresis, and analyzed using mass spectrometry. A L-gulonolactone oxidase like protein (MsGulLO) was detected, and the full length cDNA was cloned: it codes for a protein of 573 amino acids with a predicted signal peptide. MsGulLO has high similarity to L-gulonolactone oxidase 5 (AtGulLO5) in Arabidopsis thaliana, which was suggested to be involved in the pathway of ascorbate biosynthesis; however, both MsGulLO and AtGulLO5 are divergent from animal L-gulonolactone oxidases. MsGulLO was expressed mainly in flowers, and especially in nectary before blooming. However, cloning and gene expression analysis showed that L-galactonolactone dehydrogenase (MsGLDH), a vital enzyme in plant ascorbate biosynthesis, was expressed in all of flowers, roots, stems, and especially leaves. MsGulLO was purified to near homogeneity from raw MS nectar by gel filtration chromatography. The enzyme was determined to be a neutral monomeric protein with an apparent molecular mass of 70 kDa. MsGulLO is not a flavin-containing protein, and has neither L-galactonolactone dehydrogenase activity, nor the L-gulonolactone activity that is usual in animal GulLOs. However, it has weak oxidase activity with the following substrates: L-gulono-1,4-lactone, L -galactono-1,4-lactone, D-gluconic acid-δ-lactone, glucose, and fructose. MsGulLO is suggested to function in hydrogen peroxide generation in nectar but not in plant ascorbate biosynthesis.

show abstract

Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development

Cited by 24 publications

References 39 publications

Effects of ozone treatment on the antioxidant capacity of postharvest strawberry

Effects of ozone treatment on the antioxidant capacity of postharvest strawberry

Vitamin C in fleshy fruits: biosynthesis, recycling, genes, and enzymes

Characterization of a L-Gulono-1,4-Lactone Oxidase Like Protein in the Floral Nectar of Mucuna sempervirens, Fabaceae

Contact Info

Product

Resources

About