Ascorbic acid provides protection for human chondrocytes against oxidative stress

Abstract

Abstract. Oxidative stress is considered to be an important cause of dysfunction in chondrocytes and articular cartilage degradation, which leads to the pathogenesis of osteoarthritis (OA) and cartilage aging. The present study aimed to assess the effects of the widely applied antioxidant, ascorbic acid (AA), on human chondrocytes against hydrogen peroxide (H 2 O 2 ) in vitro. Using annexin V-fluorescein isothiocyanate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and senescence-associated β-galactosidase assays, the present study identified that AA reduced apoptosis, reduced the loss of viability and markedly decreased H 2 O 2 -mediated senescence in cells treated with H 2 O 2 . Furthermore, AA not only stimulated the expression levels of collagens and proteoglycans, but also inhibited the differentiation of chondrocytes under conditions of oxidative stress. In addition, reverse transcription-quantitative polymerase chain reaction and western blotting demonstrated that AA decreased the activity of nrf2, NF-κB, AP1 and matrix metalloproteinase-3, which is stimulated by H 2 O 2 . In conclusion, AA efficiently protected human chondrocytes against damage induced by H 2 O 2 by regulating multiple regulatory pathways.

Doxycycline preserves chondrocyte viability and function in human and calf articular cartilage ex vivo

“…The medium containing DMEM, MEM nonessential amino acid solution, 50 mg/ml L-ascorbic acid, selenium, insulin, transferrin, and 0.9 mmol/L sodium pyruvate was proven to maintain chondrocyte viability at levels not different from day 0 control through day 56 when storing OC graft at 37°C (Garrity et al, 2012). This may be due to the antioxidant property of L-ascorbic acid (Chang et al, 2015). Therefore, we chose the same media as Garrity et al (Garrity et al, 2012) to test if doxycycline can prolong fresh OC grafts' chondrocyte longevity in this media at 37°C.…”

“…Mitochondrial function plays an important role in modulating viability and apoptosis in chondrocytes (Chang, Huo, Li, Wu, & Zhang, 2015;Collins et al, 2016;Koike et al, 2015). Thus, we measured mitochondrial oxidative phosphorylation in chondrocytes with or without doxycycline treatment using a Seahorse XF Analyzer.…”

Taurine alleviates endoplasmic reticulum stress in the chondrocytes from patients with osteoarthritis

“…Taurine, one of the abundant amino acids existing in mammalian tissues, can resist various types of damages by its antioxidant and anti-apoptosis activities in both clinical trials and animal studies [25][26][27][28]. Taurine could not only promote cell growth and maintain phenotype of human articular chondrocytes [29], but also ameliorate ROS-induced cartilage damage through its antioxidant property [15,30]. It is worth noting that a significant increase in NRF2 mRNA levels in the H 2 O 2 -stimulated chondrocyte is observed after treatment with taurine at a low concentration of 200 μM [31].…”

Supplements und Injektionen bei Arthrose und Knorpelschäden

“…Präklinische Studien belegen für diese Vitamine und Antioxidanzien eine Schutzwirkung für den Knorpel, der durch den starken antioxidativen Effekt zu erklären ist [16,17]. Klinische Studien mit einer Kombination von Vitaminen und Spurenelementen bei Arthrose zeigen deutliche methodische Schwächen und uneinheitliche Ergebnisse.…”